Prevalence and characteristics of ESBL-producing E. coli in Dutch recreational waters influenced by wastewater treatment plants

Blaak, Hetty; Kruijf, Patrick de; Hamidjaja, Raditijo A.; Hoek, Angela H.A.M. van; Husman, Ana Maria de Roda; Schets, Franciska M.

doi:10.1016/j.vetmic.2014.03.007

Cited by 98 publications

(104 citation statements)

References 29 publications

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“…Cross-resistance of ESBLEC to other classes of antibiotics is of particular concern. A high level of cross-resistance in environmental ESBLEC was also observed in previous studies (21,22). This cross-resistance may be partly due to the coexistence of ESBL genes with other resistance genes on the same plasmids (23).…”

Section: Resultssupporting

confidence: 77%

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Gomi

Matsuda

Matsumura

et al. 2017

Antimicrob Agents Chemother

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Contamination of environmental waters by extended-spectrum-␤-lactamase (ESBL)-producing Escherichia coli (ESBLEC) is of great concern. Wastewater treatment plants (WWTPs) and hospitals release large amounts of ESBLEC into the environment. In the present study, we isolated ESBLEC strains from wastewater collected from a WWTP and a hospital in Japan and performed whole-genome sequencing to characterize these strains. Genomic analysis of 54 strains (32 from the WWTP and 22 from hospital wastewater) revealed the occurrence of clinically important clonal groups with extraintestinal pathogenic E. coli status in the WWTP and hospital wastewater. Fine-scale phylogenetic analysis was performed to further characterize 15 sequence type 131 (ST131) complex strains (11 from the WWTP and 4 from hospital wastewater). These ST131 complex strains were comprised of the following different subgroups: clade A (n ϭ 2), C1-M27 (n ϭ 8), and C1 (non-C1-M27) (n ϭ 1) for strains from the WWTP and clade A (n ϭ 2), C1-M27 (n ϭ 1), and C1 (non-C1-M27) (n ϭ 1) for strains from hospital wastewater. The results indicate that ESBLEC strains belonging to clinically important lineages, including the C1-M27 clade, may disseminate into the environment through wastewater, highlighting the need to monitor for antibiotic resistance in wastewater.

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Section: Resultssupporting

confidence: 77%

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Gomi

Matsuda

Matsumura

et al. 2017

Antimicrob Agents Chemother

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“…The findings of present study is supported by Li et al (2014) and Blaak et al (2014) who stated almost similar prevalence of E. coli in dairy waste water and recreational water. E. coli, isolated from hospital effluent showed highest resistance (ranging from 66% to 100%) against amoxicillin, cephalothin, tetracycline, sulphamethoxazole-trimethoprim, ceftriaxone, nalidixic acid and enrofloxacin.…”

Section: Discussionsupporting

confidence: 87%

“…The study revealed that the percentage of E. coli in untreated hospital effluent was 100%. Many of the previous studies indicated that the prevalence of E. coli varies widely based on type of environmental samples and it was ranging from 44% to 100% in different environmental samples such as sewage, sludge, recreational water, dairy surface water and ground water (Anastasi et al, 2010;Li et al, 2014;Blaak et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Multi-Drug Resistance Pattern of Escherichia coli Isolated from Hospital Effluent and Determination of Tetracycline Resistance Gene

Dutta¹

2016

J. Anim. Health Prod.

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“…All this resulted in widespread prevalence of antimicrobial-resistant bacteria in a broad range of environments, including soil or surface water (Baquero et al 2008). Therefore, the probability of exposure to the resistant strains of bacteria increases, since an infection might occur through contact or ingestion of contaminated surface water, for instance during recreational activities (Blaak et al 2014). This may result in both risks related to difficult in treatment infections or exposure to harmless strains of e.g., commensal-resistant bacteria, thus resulting in asymptomatic carriage of such strains (Blaak et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial resistance and prevalence of extended-spectrum beta-lactamase genes in Escherichia coli from major rivers in Podhale, southern Poland

Lenart‐Boroń

2016

Int. J. Environ. Sci. Technol.

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The aim of this study was to assess the antimicrobial resistance and the prevalence of genes determining the presence of extended-spectrum beta-lactamase (ESBL) enzymes in Escherichia coli isolated from two major rivers of the Podhale region in southern Poland. In total, 196 E. coli isolates were analyzed-98 from each river-Białka and Zakopianka, collected in 8 campaigns, over the period of two years. Antimicrobial resistance was assessed using disk diffusion method and PCR tests were conducted to detect the ESBL genes. In E. coli isolated from Białka, the resistance to amoxicillin/clavulanic acid was detected most frequently (54.08%) and ESBL was detected in 14.29% of strains. In strains isolated from Zakopianka, most frequent resistance was observed toward ticarcillin (51.02%), while ESBL was observed in 16.33% of isolates. In the total pool of isolates, the resistance to amoxicillin/clavulanic acid was most frequent (48.98% of isolates) and ESBL producers comprised 15.30% of E. coli isolates derived from both rivers. Multidrug resistance was observed less frequently in strains derived from Białka (4 isolates resistant to 10 and more antimicrobials) than from Zakopianka, where 10 isolates were resistant to 10 and more antibiotics. Out of the tested ESBL genes blaTEM was detected most frequently (45.4% of isolates), whereas blaCTX-M1 and blaCTX-M3 were recorded in one isolate.

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Prevalence and characteristics of ESBL-producing E. coli in Dutch recreational waters influenced by wastewater treatment plants

Cited by 98 publications

References 29 publications

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Multi-Drug Resistance Pattern of Escherichia coli Isolated from Hospital Effluent and Determination of Tetracycline Resistance Gene

Antimicrobial resistance and prevalence of extended-spectrum beta-lactamase genes in Escherichia coli from major rivers in Podhale, southern Poland

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