Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Gomi, Ryota; Matsuda, Tomonari; Matsumura, Yasufumi; Yamamoto, Masaki; Tanaka, Michio; Ichiyama, Satoshi; Yoneda, Minoru

doi:10.1128/aac.00564-17

Cited by 48 publications

(39 citation statements)

References 51 publications

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“…In this 2-month survey, the incidence rate of ESBL-producing Enterobacteriaceae from urine collected from patients hospitalized in three health care facilities from two geographical areas of Tunisia, ranged from 11.1% to 3.7%. Data are consistent with those described in literature which showed variable incidence rates depending of risk factors of ESBL-E acquisition (17, 18). Few studies including molecular analysis ESBL-E from community-acquired infections were reported in Tunisia (16).…”

Section: Discussionsupporting

confidence: 92%

Emergence of B2-ST131-C2 and A-ST617 Escherichia coli clones producing both CTX-M-15- and CTX-M-27 and ST147 NDM-1 positive Klebsiella pneumoniae in the Tunisian community

Mhaya

Trabelsi

Bégu

et al. 2019

Preprint

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25CTX-M ESBLs have been increasingly reported from human enterobacteria isolates in 26 Tunisia. NDM-1 carbapenemase was recently reported in isolates from Tunisian Hospitals. 27 During a 2-month period (December 2017 to January 2018), we have collected 23 ESBL- 28 producing Enterobacteriaceae (ESBL-E) from urines of patients hospitalized in three health 29 care facilities and from community, situated in two distinct Tunisian geographical regions. 30 They were divided into 15 Escherichia coli and eight Klebsiella pneumoniae. The aim of this 31 study was to characterize ESBL-E with regard to their β-lactamase content and their 32 epidemiological relationship. The results indicated a high rate (47%) of E. coli producing both 33 CTX-M group-1 and -9. For the first time, we demonstrated the presence of E. coli having 34 concomitantly CTX-M-15 and CTX-M-27 which belong to two sequences types (ST), ie. A-35 ST617 (2 isolates) and B2-ST131 subclade C2 (2 isolates). All four E. coli isolates carried a 36 multireplicon IncF with an identical allelic combination, F31:A4:B1. This study reports also 37 the first description of K. pneumoniae belonging to the clone ST147 carrying the 38 carbapenemase NDM-1 in the Tunisian community. In conclusion, our data confirms the need 39 for monitoring the resistance to extended-spectrum cephalosporins and to carbapenems 40 among enterobacteria in Tunisia. 41 42 43Resistance to extended-spectrum cephalosporins (ESC) is widespread among 44 Enterobacteriaceae species and is mainly due to the production of extended-spectrum ß-45 lactamases (ESBLs). The dissemination of ESBLs is become a growing concern since they 46 are considered as a major cause of morbidity and mortality. Up to the end of the 1990s, TEM-47 and SHV-type ESBLs were mainly produced by the Klebsiella pneumoniae and Enterobacter 48 spp. responsible for nosocomial infections (1, 2). Since 2000s, CTX-M ESBLs have gained 49 prominence mainly in Escherichia coli and K. pneumoniae strains and are now considered as 50 pandemic enzymes. Many bla CTX-M variants exist, but they can be divided into two main 51 clusters (groups -1 and -9); each cluster of CTX-M genotypes has a corresponding progenitor 52 gene sharing homology with different environmental Kluyvera spp. from which bla CTX-M 53 genes originated. Among CTX-M enzymes, CTX-M-15 belonging to group 1, has currently 54 been the most frequent all over the world (1, 3) 55 The majority of CTX-M type ESBL-associated E. coli infections is often concentrated within 56 specific extraintestinal pathogenic E. coli (ExPEC) lineages belonging to highly virulent 57 phylogenetic group B2, and recognizable by their sequence type (ST). The bla CTX-M-15 is the 58 dominant ESBL gene in the virulent E. coli ST131 clone, in particular in the subclade ST131-59 C2 (4). However other genetically divergent CTX-M genes also occur in this ST, such as 60 bla CTX-M-14/14-like variants in Canada, China, and Spain (5, 6). A subclade of E. coli ST131 61 producing the CTX-M-27 (group 9 enzyme, CTX-M-1...

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Section: Discussionsupporting

confidence: 92%

Emergence of B2-ST131-C2 and A-ST617 Escherichia coli clones producing both CTX-M-15- and CTX-M-27 and ST147 NDM-1 positive Klebsiella pneumoniae in the Tunisian community

Mhaya

Trabelsi

Bégu

et al. 2019

Preprint

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“…The rate of ESBL producing Enterobacteriacea in our work were within the range of previous studies. It was considerably high in comparison to some studies of WWTP effluent (0.5-9.8%) (34, 80-83). However it was lower than those studies in wastewater (45-100%) (84, 85).…”

Section: Discussioncontrasting

confidence: 57%

Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

Smyth

O’Flaherty

Walsh

et al. 2019

Preprint

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26Wastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and 27 spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this 28 work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms 29 of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were 30 collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial 31 susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out 32 to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL 33 genes were detected by PCR. Plasmids carrying ESBL genes were transformed into 34Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify 35 incompatibility groups. More than 90% of isolated faecal coliforms were resistant to 36 amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 37 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem 38 were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a 39 multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of 40 isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried 41 blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL 42 isolates were successfully transformed into recipient E. coli. The detected plasmid 43 incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results 44 provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and 45 are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae. 46 47 48 49 Keywords 50 Antibiotic resistance, multidrug resistance, AmpC, extended-spectrum β-lactamase (ESBL), 51 plasmids 52 53 54 55 56 3

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“…Many exist as harmless commensals in the human gut, but some are classified as intra-(InPEC) or extra-intestinal pathogenic Escherichia coli (ExPEC 18 ). Based on their virulence genes profile the pathogenic potential of Escherichia coli isolates can be determined 7 . The sequenced isolates contain some 700 of the 2000 Escherichia coli virulence factors in the virulence factor database 19 , averaging to 153 and to 155 virulence factors per isolate for inflow and outflow, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…At the heart of modern resistance development is a human-centred network of clinics, industry, private homes, farming, and wastewater. Recent studies suggest that wastewater contains a significant amount of antibiotic resistant Escherichia coli , specifically extended-spectrum beta-lactamase-producing Escherichia coli 7 . Particularly, multidrug-resistant (MDR) clones (normally defined as those resistant to three or more drug classes) are of great concern.…”

Section: Introductionmentioning

confidence: 99%

High genomic diversity of multi-drug resistant wastewater Escherichia coli

Mahfouz

Caucci

Achatz

et al. 2017

Preprint

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19Wastewater treatment plants play an important role in the release of antibiotic resistance into the 20 environment. It has been shown that wastewater contains multi-drug resistant Escherichia coli, 21 but information on strain diversity is surprisingly scarce. Here we present an exceptionally large 22 dataset on multidrug resistant Escherichia coli, originating from wastewater, over a thousand 23 isolates were phenotypically characterized for twenty antibiotics and for 103 isolates whole 24 genomes were sequenced. To our knowledge this is the first study documenting such a 25 comprehensive diversity of multi-drug resistant Escherichia coli in wastewater. The genomic 26 diversity of the isolates was unexpectedly high and contained a high number of resistance and 27 virulence genes. To illustrate the genomic diversity of the isolates we calculated the pan genome 28 of the wastewater Escherichia coli and found it to contain over sixteen thousand genes. To 29 analyse this diverse dataset, we devised a computational approach correlating genotypic variation 30 and resistance phenotype, this way we were able to identify not only known, but also candidate 31 resistance genes. Finally, we could verify that the effluent of a wastewater treatment plant will 32

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Occurrence of Clinically Important Lineages, Including the Sequence Type 131 C1-M27 Subclone, among Extended-Spectrum-β-Lactamase-Producing Escherichia coli in Wastewater

Cited by 48 publications

References 51 publications

Emergence of B2-ST131-C2 and A-ST617 Escherichia coli clones producing both CTX-M-15- and CTX-M-27 and ST147 NDM-1 positive Klebsiella pneumoniae in the Tunisian community

Emergence of B2-ST131-C2 and A-ST617 Escherichia coli clones producing both CTX-M-15- and CTX-M-27 and ST147 NDM-1 positive Klebsiella pneumoniae in the Tunisian community

Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

High genomic diversity of multi-drug resistant wastewater Escherichia coli

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