Pretreatment of seeds with thidiazuron delimits its negative effects on explants and promotes regeneration in chickpea (Cicer arietinum L.)

Kumari, Pragati; Singh, Sumer; Yadav, Saurabh; Tran, Lam‐Son Phan

doi:10.1007/s11240-017-1365-6

Cited by 25 publications

(12 citation statements)

References 57 publications

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“…Generally, cytokinin was found to be essential for shoot induction and proliferation; however, its type and suitable concentration varies with the species (Tubic et al 2016;Kumari et al 2018). TDZ produced superior responses for cotyledonary nodes regeneration than the other cytokinins (6-BA, KT; data not shown) in our study, which might be related to its concentration being appropriate for stimulating endogenous hormones metabolism and inducing the production of endogenous hormones for the induction of multiple shoots.…”

Section: Discussionmentioning

confidence: 63%

“…TDZ produced superior responses for cotyledonary nodes regeneration than the other cytokinins (6-BA, KT; data not shown) in our study, which might be related to its concentration being appropriate for stimulating endogenous hormones metabolism and inducing the production of endogenous hormones for the induction of multiple shoots. TDZ is one of the most effective plant growth regulators and a derivative of phenyl urea (Kumari et al, 2018), and it is believed to be the best synthetic cytokinin present for the regeneration of numerous plant systems, including Salvia (Sivanesan et al 2011). TDZ promotes axillary bud proliferation via reducing the dominance of the apical meristem (Silué, et al, 2016;Thomas and Katterman 1986).…”

Section: Discussionmentioning

confidence: 99%

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In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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Background: As a highly valued medicinal plant, Salvia plebeia R. Brown belongs to the Lamiaceae family that has been subjected to over exploitation in its natural habitat for phytochemical and pharmacological studies. Alternative collection methods need to be developed for the large-scale propagation of Salvia plebeian.Results: Here, e cient and simple, direct organogenesis (from shoot tips and cotyledonary nodes explants) and Globular bodies (GBs) induction (from hypocotyl explants) systems were developed for the in vitro propagation of Salvia plebeia. The highest and number of regenerated shoots (7.0±0.82) per shoot tips was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 0.1 mg L -1 indole-3-acetic acid (IAA) and 1.0 mg L -1 6-benzyladenine (6-BA), the proliferation of shoots and shoots rooted were carried out on the same medium treatments almost synchronously. Similarly, MS medium supplemented with 0.1 mg L -1 IAA and 1.0 mg L -1 thidiazuron (TDZ) yielded the maximum number of shoots (37.5±1.34) with 100% shoot sprouting frequency. Simultaneously, a protocol was developed for GBs induction from hypocotyl explants, and it produced 17.4 GBs per explant with 82.7% response on MS medium supplemented with TDZ (1.0 mg L -1 ) and IAA (0.1 mg L -1 ), and produced GBs that were morphologically similar to globular embryos and successfully germinated on hormone-free MS medium. The acclimatized plantlets with well-developed root systems were successfully shifted to the natural soils with a 100% survival rate.Conclusions: Taken together, this protocol can be e ciently used for mass propagation, germplasm preservation and likely also for gene transfer of Salvia plebeia.

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Section: Discussionmentioning

confidence: 63%

Section: Discussionmentioning

confidence: 99%

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

Cheng

Yang

et al. 2021

In Vitro Cell.Dev.Biol.-Plant

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“…BA was the most effective among the four cytokinins employed in axillary shoot regeneration in A. lupulina similar to that reported by Sivanesan and Park [ 18 ]. TDZ is believed to be the best synthetic cytokinin present for the regeneration of numerous plant systems [ 19 , 20 ]. High cytokinin activity of TDZ inhibits shoot elongation; however, it is conducive to the formation of somatic embryos [ 15 , 21 ].…”

Section: Discussionmentioning

confidence: 99%

“…Our observations are in line with high-cytokinin emerging species beneficial for the somatic embryogenesis induction [ 21 , 24 ]. However, long exposure of high concentration TDZ resulted in browning, deformation of seedlings, necrosis, and, subsequently, death [ 20 , 29 ]. However, here, we found leaves containing BA brown but not TDZ, which may be due to different responses of different species to different cytokinins.…”

Section: Discussionmentioning

confidence: 99%

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Yang

et al. 2021

Plants

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This work was aimed at establishing an effective approach for in vitro propagation of Ajuga lupulina Maxim, a medicinal and ornamental plant mainly found in eastern Xizang, in the western Sichuan region of China. We report an optimum response in the proliferation of axillary shoots from nodal segment explants (10.2 shoots/explant) on MS medium containing 3.0 mg L−1 of 6-benzyladenine (BA). When BA and TDZ individually or in combination with NAA were employed for adventitious shoot regeneration, shoots and embryo-like structures (ELSs) were noted in the callus from leaf explants. The maximum response of 26.4 shoots /explant (81.6%) and 12.0 ELSs/explant were ascertained on MS medium with 4.0 mg L−1 TDZ and 0.1 mg L−1 NAA. The leaf despite browning still demonstrated a high regeneration capacity. TDZ (2.0 mg L−1) and BA (2.0 mg L−1) along with NAA (0.01 mg L−1) were found to perform well for shoot regeneration via callus from shoot tip explants. The best for rooting was MS medium (half-strength) containing indole-3-butyric acid (IBA: 1.5 mg L−1) and (NAA: 0.5 mg L−1) with the maximum number of roots (25.8 per shoot) and the highest rooting frequency (81.71%). The survival of the plantlets in the greenhouse was 78.2% indicative of successful acclimatization. This work is the first report of a consistent, definitive, and unique protocol for A. lupulina regeneration, paving the way for the in vitro preservation of such significant genetic resources and also further allied systems based on such callus-based or embryo-based approaches.

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“…Although these methods increase root efficiency in chickpea, nonetheless, they are difficult to handle and are time consuming. A reliable chickpea regeneration system has not been available yet, because of the fact that it owns recalcitrant nature to regeneration [ 60 ]. Iron is used as an enzyme cofactor and being an indispensable element for the photosynthetic process.…”

Section: Resultsmentioning

confidence: 99%

Biogenic iron oxide nanoparticles enhance callogenesis and regeneration pattern of recalcitrant Cicer arietinum L.

et al. 2020

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This study is the first report on the biosynthesized iron oxide nanoparticles (IONPs) which mediate in-vitro callus induction and shoot regeneration in economically important recalcitrant chickpea crop (Cicer arietinum L.). Here, we used leaf extract of Cymbopogon jwarancusa for the synthesis of IONPs in order to achieve a better biocompatibility. The bioactive compounds in C. jwarancusa leaf extract served as both reducing and capping agents in the fabrication process of IONPs. Field emission scanning electron microscopy (FE-SEM) revealed rods like surface morphology of IONPs with an average diameter of 50±0.2 nm. Energy-dispersive X-ray spectroscopy (EDS) depicted formation of pure IONPs with 69.84% Fe and 30.16% O2. X-ray diffractometry (XRD) and attenuated total reflectance-fourier transform infrared (ATR-FTIR) validate the crystalline structure, chemical analysis detect the presence of various biomolecular fingerprints in the as synthesized IONPs. UV-visible absorption spectroscopy depicts activity of IONPs under visible light. Thermo-gravimetric analysis (TGA) displayed thermal loss of organic capping around 500°C and confirmed their stabilization. The biosynthesized IONPs revealed promising results in callus induction, shoot regeneration and root induction of chickpea plants. Both chickpea varieties Punjab-Noor 09 and Bittle-98 explants, Embryo axes (EA) and Embryo axes plus adjacent part of cotyledon (EXC) demonstrated dose-dependent response. Among all explants, EXC of Punjab-Noor variety showed the highest callogenesis (96%) and shoot regeneration frequency (88%), while root induction frequency was also increased to 83%. Iron content was quantified in regenerated chickpea varieties through inductively coupled plasma-optical emission spectrometry. The quantity of iron is significantly increased in Punjab-Noor regenerated plants (4.88 mg/g) as compare to control treated plants (2.42 mg/g). We found that IONPs enhance chickpea growth pattern and keep regenerated plantlets infection free by providing an optimum environment for rapid growth and development. Thus, IONPs synthesized through green process can be utilized in tissue culture studies in other important recalcitrant legumes crops.

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Pretreatment of seeds with thidiazuron delimits its negative effects on explants and promotes regeneration in chickpea (Cicer arietinum L.)

Cited by 25 publications

References 57 publications

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

In vitro propagation via organogenesis and formation of globular bodies of Salvia plebeia: a valuable medicinal plant

A Novel and Efficient In Vitro Organogenesis Approach for Ajuga lupulina Maxim

Biogenic iron oxide nanoparticles enhance callogenesis and regeneration pattern of recalcitrant Cicer arietinum L.

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