Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows’ milk

Samsonova, J.V.; Safronova, V.A.; Осипов, А. И.

doi:10.1016/j.talanta.2014.10.043

Cited by 47 publications

(29 citation statements)

References 22 publications

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“…Ascites fluids were generated in paraffin primed BALB/c mice. The subclass of the isotypes of the antibody was determined by using a commercialized mouse monoclonal antibody isotyping kit (Samsonova, Safronova, & Osipov, 2015). Monoclonal antibody affinity (Ka) was measured following with a classical noncompetitive enzyme immunoassay described by Batty et al (Samsonova et al, 2015).…”

Section: Cell Fusion and Hybridoma Screeningmentioning

confidence: 99%

Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

Yang

Wang

Song

et al. 2015

LWT - Food Science and Technology

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Section: Cell Fusion and Hybridoma Screeningmentioning

confidence: 99%

Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

Yang

Wang

Song

et al. 2015

LWT - Food Science and Technology

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“…Lateral flow (LF) biosensors have been applied to detect a great variety of species, including biomolecules, pathogens, contaminants, and allergens . Examples include detecting Escherichia coli , progesterone in milk, and the allergen parvalbumin using magnetic nanoparticles . In addition, researchers continue to improve LFAs by augmenting their detection capabilities, developing methods such as enzyme‐amplified LFAs for E. coli detection and LFAs improved by isotachophoresis …”

Section: Introductionmentioning

confidence: 99%

Aminolated and Thiolated PEG‐Covered Gold Nanoparticles with High Stability and Antiaggregation for Lateral Flow Detection of Bisphenol A

Lin

Uzunoğlu

Stanciu

2017

Small

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The few lateral flow assays (LFAs) established for detecting the endocrine disrupting chemical bisphenol A (BPA) have employed citrate-stabilized gold nanoparticles (GNPs), which have inevitable limitations and instability issues. To address these limitations, a more stable and more sensitive biosensor is developed by designing strategies for modifying the surfaces of GNPs with polyethylene glycol and then testing their effectiveness and sensitivity toward BPA in an LFA. Without the application of any enhancement strategy, this modified BPA LFA can achieve a naked-eye limit of detection (LOD) of 0.8 ng mL , which is 12.5 times better than the LOD of regular BPA LFAs, and a quantitative LOD of 0.472 ng mL . This modified LFA has the potential to be applied to the detection of various antigens.

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“…These techniques require laborious sample preparation, high costs, and skilled personnel trained to use the benchtop equipment to generate the results. Read-out times, sensitivity, and specificity are some disadvantages of lateral flow technology [16,17,18] and ELISA kits [19,20], which have been used to detect progesterone in milk. Recently, Daems et al [21] developed a Surface Plasmon Resonance (SPR) biosensor to detect progesterone in dairy milk as a competitive inhibition assay with a limit of detection of 0.5 ng/mL.…”

Section: Introductionmentioning

confidence: 99%

GryphSens: A Smartphone-Based Portable Diagnostic Reader for the Rapid Detection of Progesterone in Milk

Jang

Ahmed

Neethirajan

2017

Sensors

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Enzyme-linked immunosorbent assay (ELISA) is a popular assay technique for the detection and quantification of various biological substances due its high sensitivity and specificity. More often, it requires large and expensive laboratory instruments, which makes it difficult to conduct when the tests must be performed quickly at the point-of-care (POC). To increase portability and ease of use, we propose a portable diagnostic system based on a Raspberry Pi imaging sensor for the rapid detection of progesterone in milk samples. We designed, assembled, and tested a standalone portable diagnostic reader and validated it for progesterone detection against a standard ELISA assay using a commercial plate reader. The portable POC device yielded consistent results, regardless of differences in the cameras and flashlights between various smartphone devices. An Android application was built to provide front-end access to users, control the diagnostic reader, and display and store the progesterone measurement on the smartphone. The diagnostic reader takes images of the samples, reads the pixel values, processes the results, and presents the results on the handheld device. The proposed POC reader can perform to superior levels of performance as a plate reader, while adding the desirable qualities of portability and ease of use.

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Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows’ milk

Cited by 47 publications

References 22 publications

Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

Aminolated and Thiolated PEG‐Covered Gold Nanoparticles with High Stability and Antiaggregation for Lateral Flow Detection of Bisphenol A

GryphSens: A Smartphone-Based Portable Diagnostic Reader for the Rapid Detection of Progesterone in Milk

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