Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

Yang, Xing-Dong; Wang, Fangyu; Song, Chao; Wu, Shuyan; Zhang, Gaiping; Zeng, Xianyin

doi:10.1016/j.lwt.2015.04.022

Cited by 33 publications

(18 citation statements)

References 30 publications

(31 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the polyclonal antibody was selected to develop the icELISA to detect E 2 in milk samples in term of lower IC 50 values. Our results indicated that the sensitivity of the polyclonal antibody produced in this study was among the best reported (Yang et al, 2015). The specificity of the polyclonal antibody was evaluated by comparing the IC 50 values of other structurally related compounds to that of E 2 as expressed by CR values ( Table 3).…”

Section: Development Of Icelisa In Milkmentioning

confidence: 59%

“…Actually, there were reports showing that the antibody obtained from at 3-position were more sensitive than that obtained from 17-position of E 2 . Watanabe, Kubo, Kanzaki, and Nakazawa (2010) previously prepared β-estradiol 17-hemisuccuinate as a hapten for producing polyclonal antibodies with an IC 50 values of 6.9 ng/mL, and Yang et al (2015) synthesized E 2 -CME as a hapten for conjugating carrier proteins, obtaining a monoclonal antibody specific for E 2 with an IC 50 value of 0.18 ng/mL using a lateral-flow colloidal gold immunoassay strip. Thus, we synthesized the E 2 -CME used as hapten to produce antibodies to E 2 in this study.…”

Section: Preparation Of Haptens and Conjugatesmentioning

confidence: 99%

“…Therefore, many countries have banned E 2 use in the livestock and poultry industries. Milk is considered a perfect natural food for people; however, the illegal and uncontrolled use of E 2 in dairy cattle could cause a wide range of health problems (Chen, Mi, et al, 2014;Yang et al, 2015). Therefore, it is of great significance to establish a rapid and sensitive method for monitoring E 2 residue in milk.…”

Section: Introductionmentioning

confidence: 99%

“…This technique provides many advantages, including rapid, simple operation, and high-throughput capabilities . Antibody represents the key reagent in immunoassays, and some E 2 -specific antibodies have been reported (Caron, Sheedy, & Farenhorst, 2010;Li & Jiang, 2012;Yang et al, 2015). However, the sensitivities of some of these antibodies are too low [half maximum inhibition concentration (IC 50 ) = 243 ng/mL (Caron et al, 2010) or 3.76 ng/mL (Li & Jiang, 2012)] to be used for establishing a highly sensitive icELISA to detect traces of E 2 residue in milk.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

Bai

Liu

et al. 2017

Food and Agricultural Immunology

View full text Add to dashboard Cite

The residue of 17β-estradiol (E 2) in milk could potentially lead to the occurrence of various reproductive diseases; therefore, a rapid and sensitive method for monitoring E 2 residues in milk was highly necessary. In this study, we produced new polyclonal and monoclonal antibodies using E 2-3-O-carboxymethyl ether as a hapten and developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) for the detection of E 2 in milk. The results showed that the sensitivity of polyclonal antibody was higher than that of monoclonal antibody, providing a half maximum inhibition concentration (IC 50) against E 2 of 0.17 ng/mL, high cross-reactivity (CR) to E 2 benzoate (150%) and oestriol (18.02%), and negligible CR with other oestrogen compounds. Under optimized conditions, the developed icELISA based on the polyclonal antibody had a limit of detection values of 0.093 μg/L, which was enough sensitive to detect E 2 in milk. In spiked samples (0.5, 1, and 2 μg/L), the recoveries ranged from 83.12% to 94.58% with coefficients of variation <12.8%. These results indicated that the icELISA method we developed was suitable for screening of E 2 residue in milk.

show abstract

Section: Development Of Icelisa In Milkmentioning

confidence: 59%

Section: Preparation Of Haptens and Conjugatesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

Bai

Liu

et al. 2017

Food and Agricultural Immunology

View full text Add to dashboard Cite

show abstract

“…A polyvinyl chloride (PVC) backing card, an nitrocellulose (NC) membrane, the sample pad and absorption pad were assembled to prepare the lateral-flow ICA strip as described previously (Yang et al, 2015). Sample and absorbent pads were made from nonwoven, 100% pure cellulose fiber.…”

Section: Sensitivity and Specificity Of The Assaymentioning

confidence: 99%

Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

Li¹,

AiMin²,

Jia

et al. 2017

Food and Agricultural Immunology

View full text Add to dashboard Cite

In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cutoff value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.

show abstract

Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip

Xu¹,

Sun²,

He³

et al. 2021

Food Science & Nutrition

View full text Add to dashboard Cite

An immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneously rapid and sensitive detection of diethylstilbestrol (DES) and estradiol (E2) in milk and tissue samples. For this purpose, two monoclonal antibodies raised against those two estrogens were conjugated to gold nanoparticles and were applied to the conjugate pads of the test strip. The competitors of the DES‐BSA/E2‐BSA conjugates were immobilized onto a nitrocellulose membrane at two detection zones to form T1 and T2, respectively. The immunochromatographic assay had a visual detection limit of DES at 30 ng/g in milk powder, 25 ng/g in liquid milk, and 25 ng/g in shrimp tissue, respectively, and the results can be judged within 7–10 min. The visual detection limit of E2 was 75 ng/g in milk powder, 65 ng/g in liquid milk, and 60 ng/g in shrimp tissue, respectively, and the results can be judged within 3–4 min. It had advantages in easy operation without requiring sophisticated equipment and specialized skills. By testing thirty milk and shrimp tissue samples from the local market, the method was compared with the HPLC‐MS / MS method, and there was no statistical difference between the two methods. Furthermore, the immunochromatographic assay had good specificity, simple procedure, and low cost. This protocol was well suited for the food safety monitoring and early warning.

show abstract

Establishment of a lateral flow colloidal gold immunoassay strip for the rapid detection of estradiol in milk samples

Cited by 33 publications

References 30 publications

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

Production of antibodies and development of an enzyme-linked immunosorbent assay for 17β-estradiol in milk

Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

Simultaneous detection of diethylstilbestrol and estradiol residues with a single immunochromatographic assay strip

Contact Info

Product

Resources

About