Pressurized capillary electrochromatographic analysis of water-soluble vitamins by combining with on-line concentration technique

Li, Jia; Liu, Yaling; Du, Yanyan; Xing, Da

doi:10.1016/j.chroma.2007.03.080

Cited by 21 publications

(16 citation statements)

References 36 publications

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“…The sample preparation procedure was similar to the method described previously [20]. Briefly, weigh 120 mg corn sample accurately (Sartorius CP224S analytical balance, Goettingen, Germany) and put it into a 15 mL plastic tube.…”

Section: Sample Preparationmentioning

confidence: 99%

Capillary liquid chromatographic analysis of fat-soluble vitamins and β-carotene in combination with in-tube solid-phase microextraction

2009

Journal of Chromatography B

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Section: Sample Preparationmentioning

confidence: 99%

Capillary liquid chromatographic analysis of fat-soluble vitamins and β-carotene in combination with in-tube solid-phase microextraction

2009

Journal of Chromatography B

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“…The phytohormones extraction procedure was similar to the method described previously [19]. Briefly, 4 mL of cold methanol ( -208C) was added to the tube containing the corn sample (120 mg) and incubated at -208C for 30 min.…”

Section: Sample Preparationmentioning

confidence: 99%

On‐line concentration and pressurized capillary electrochromatographic analysis of phytohormones in corn

Wang

Li²,

Xing

et al. 2008

J of Separation Science

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A pressurized CEC (pCEC) method was developed for the separation of phytohormones, in which UV absorbance was used as the detector and a monolithic silica-ODS column as the separation column. The parameters (including the concentration of organic solvent in the mobile phase, pH of the electrolyte buffer, applied voltage) affecting the separation resolution were evaluated. Two on-line concentration techniques, namely, solvent gradient zone sharpening effect and field-enhanced sample stacking, were utilized to improve detection sensitivity. The combination of the two techniques proved to be beneficial to enhance the detection sensitivity by enabling the injection of large volumes of samples. Compared to the conventional injection mode, the enhancement in the detection sensitivities of phytohormones using the on-line concentration technique is in the range from 9- to 23-fold. The developed pCEC method was applied to evaluate phytohormones in corns.

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“…Jia et al 84 apresentaram um método de determinação de vitaminas hidrossolúveis, empregando pré-concentração on-line e utilizando um sistema de pCEC, com detecção no UV e coluna monolítica de sílica-C18 como capilar de separação. Existem vários métodos que podem ser empregados para a determinação de vitaminas solúveis em água, incluindo HPLC e CE, e a maioria deles é problemática por se tratar de uma separação de amostra complexa.…”

Section: Alimentosunclassified

Eletrocromatografia capilar: contextualização, estado da arte e perspectivas

Segato¹,

Silva²,

Jardim³

2009

Quím. Nova

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ElEtrocromatografia capilar: contExtualização, Estado da artE E pErspEctivas milena pinotti segato, césar ricardo silva e isabel cristina sales fontes Jardim* Instituto de Química, Universidade Estadual de Campinas, CP 6154, 13084-971 Campinas -SP, BrasilRecebido em 25/3/08; aceito em 21/8/08; publiado na web em 26/1/09 CAPILLARY ELECTROCHROMATOGRAPHY: CONTEXTUALIZATION, STATE-OF-THE-ART AND PERSPECTIVES. Capillary electrochromatography (CEC) is a separation technique in which the mobile phase flow is based on the application of a voltage across a packed capillary, which generates an electroosmotic flow that transports the analytes along the capillary toward the detector. As it combines the separation mechanisms of high-performance liquid chromatography (HPLC) and of capillary electrophoresis (CE), CEC can be considered a hybrid of HPLC and CE. This review presents some fundamental aspects of CEC and is focused on the instrumental advances of the technique, such as column technology, operation modes and detection systems, presenting recent papers on these topics and some applications and perspectives about CEC.

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Pressurized capillary electrochromatographic analysis of water-soluble vitamins by combining with on-line concentration technique

Cited by 21 publications

References 36 publications

Capillary liquid chromatographic analysis of fat-soluble vitamins and β-carotene in combination with in-tube solid-phase microextraction

Capillary liquid chromatographic analysis of fat-soluble vitamins and β-carotene in combination with in-tube solid-phase microextraction

On‐line concentration and pressurized capillary electrochromatographic analysis of phytohormones in corn

Eletrocromatografia capilar: contextualização, estado da arte e perspectivas

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