Pressure-assisted capillary electrophoresis–electrospray ion trap mass spectrometry for the analysis of heparin depolymerised disaccharides

Ruiz-Calero, Victoria; Moyano, Encarnación; Puignou, L.; Galcerán, M.T.

doi:10.1016/s0021-9673(00)01181-x

Cited by 66 publications

(56 citation statements)

References 32 publications

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“…In both articles [71,72] the DUA(1 ? 4)a-D-GlcNAc (IVA) disaccharide migration was boosted by pressure application at the capillary inlet whenever the analysis was performed in the negative separation mode.…”

Section: Gagsmentioning

confidence: 99%

“…To date, the main articles regarding the CE-MS of saccharidic compounds found in literature include the analysis of (i) standard mixtures [42,[47][48][49][50][51][52]; (ii) carbohydrates in nutrition [19,[53][54][55][56][57][58][59]; (iii) glycans from glycoproteins [60][61][62][63][64][65]; (iv) carbohydrates in drug development [45,[66][67][68][69][70][71][72][73][74][75][76][77][78][79][80][81].…”

Section: Introductionmentioning

confidence: 99%

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Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

et al. 2006

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Overview on advances in capillary electrophoresis-mass spectrometry of carbohydrates: A tabulated reviewThe increasing interest for carbohydrates as holder of essential bioinformations has boosted their full characterization through analytical techniques. The intent of this review is to summarize the recent trends regarding on-line and off-line CE-MS coupling for carbohydrate analysis. A statistical survey on the articles that use derivatizing agents as well as on the analyzer and type of instrument coupling (i.e. on-or off-line) is depicted. From a general overview it can be concluded that, whereas derivatization might be useful for the detection of neutral carbohydrates improving separation selectivity with volatile buffers and increasing sensitivity of the MS detection, relatively few works with derivatized carbohydrates were found; this was noticed in particular for glycosides and saccharides carrying ionizable groups, which are normally analyzed without any chemical modification. The most applied coupling is the on-line sheath-liquid interface; for online applications, ESI is the sole source used, whilst the most common analyzer is the IT. MS n is often exploited, as fragmentation increases the achieved structural information. CE-MS turned out to be mainly used for the analysis of carbohydrates in drug development (i.e. study of oligosaccharides from pathogens, carbohydrate-based drugs and drug metabolites), in nutrition and for characterization of glycans from glycoproteins. The reader will find elucidating tables regarding these recent CE-MS applications, including the main information on the analysis conditions. Comments are meant to help the immediate focus on the usefulness of the analytical technique and predict the difficulties found during analysis and, in case, their overcoming.

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Section: Gagsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

et al. 2006

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“…These polysaccharides are linear, with highly sulfated chains and extremely heterogeneous in structure [2]. Heparin, a highly sulfated variant, composes of repeating disaccharide units consisting of hexuronic acids linked to glucosamine units by ␣(1 → 4) bonds [3]. It is well known that heparin interacts with many biologically important proteins such as proteases inhibitors, extracellular signaling molecules, lipid-or membrane-binding proteins and adhesion proteins.…”

Section: Introductionmentioning

confidence: 99%

Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2

Liang

et al. 2005

Journal of Pharmaceutical and Biomedical Analysis

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“…The application of pressure at the inlet capillary avoids bubbling and produces an additional flow arising from the pressure difference between the capillary ends. Although this approach has been endorsed by some authors [1,6], the combination of pressure-assisted capillary chromatography with mass spectrometric detection (pCEC-MS) requires deeper investigation with a view to identifying its actual advantages and disadvantages.…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of aliphatic amines in water samples by pressure‐assisted monolithic octadecylsilica capillary electrochromatography‐mass spectrometry

et al. 2004

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A pressure-assisted capillary chromatography-mass spectrometry method based on the use of a monolithic octadecylsilica (ODS) capillary is proposed for the determination of aliphatic amines. A 25 mM citric acid buffer containing 10% methanol is used as running electrolyte. Separation is achieved by simultaneously applying a capillary electrophoresis (CE) voltage of 13 kV and an overimposed pressure of 8 bar. The use of pressure is required to ensure stable electrospray conditions. Analysis times are reduced by using a capillary column consisting of a 30 cm long monolithic silica capillary column bound with ODS and a fused-silica capillary column also 30 cm long. The proposed method was successfully applied to the determination of low-molecular-weight aliphatic amines in tap and river water. The analysis of real samples requires cleanup and preconcentration, which can be performed automatically by inserting a minicolumn in the replenishment system of the commercial instrument.

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Pressure-assisted capillary electrophoresis–electrospray ion trap mass spectrometry for the analysis of heparin depolymerised disaccharides

Cited by 66 publications

References 32 publications

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

Overview on advances in capillary electrophoresis‐mass spectrometry of carbohydrates: A tabulated review

Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2

Rapid determination of aliphatic amines in water samples by pressure‐assisted monolithic octadecylsilica capillary electrochromatography‐mass spectrometry

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