Capillary zone electrophoresis characterization of low molecular weight heparin binding to interleukin 2

Determination of dissociation constants between polyelectrolytes and proteins by affinity capillary electrophoresis

“…and interleukin 2 (IL-2) [21], between folate-binding proteins and heparin [22], between LMMH and granulocyte-colony stimulating factor [23], and also between heparin-like glycosaminoglycans and the (96-110) heparin-binding domain of amyloid precursor protein [24].…”

Pre-equilibrium capillary zone electrophoresis or frontal analysis: Advantages of plateau peak conditions in affinity capillary electrophoresis

“…However, interactions characterized by rapid on-and-off kinetics have relatively recently also been studied using pre-equilibrium CZE conditions [10][11][12][13][14], i.e., under experimental conditions where plateau peaks were not produced. Due to zone-broadening the determination of free analyte concentrations was based on peak heights rather than on peak areas in four of these studies [11][12][13][14]. The peak areas in the four studies were reported to be constant [11,13] or nearly constant [12,14] and not useful for estimation of the degree of binding.…”

“…Due to zone-broadening the determination of free analyte concentrations was based on peak heights rather than on peak areas in four of these studies [11][12][13][14]. The peak areas in the four studies were reported to be constant [11,13] or nearly constant [12,14] and not useful for estimation of the degree of binding.…”

Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis

“…This is a unique feature as compared with other techniques that were used for the study of noncovalent interactions [15]. CE is the most appropriate method for both the assay of strong binding systems (the complex will not dissociate during the required separation time period) and the assay of weak binding systems [18]. There have been many studies on the interactions between nevirapine and HIV RT of their binding site simulation by computational chemistry in the allosteric nonsubstrate binding pocket site [2,[19][20][21], but there is no literature reported on the apparent binding constants of them.…”