Determination of dissociation constants between polyelectrolytes and proteins by affinity capillary electrophoresis

Complex coacervates of hyaluronic acid and lysozyme: Effect on protein structure and physical stability

“…Samples containing BSA instead of LZ were also prepared for solution depletion experiments and as expected showed no visible complex coacervation upon mixing in any ratio as well as no reduction of the BSA concentration measured in the supernatant (data not shown), indicating that no macroscopic complex coacervates are formed between the negatively charged polymer and protein. Note that this does not exclude the formation of soluble complexes which could be present as indicated by Lenormand et al [18] and the work of Anderot et al [2], which shows interactions between HSA and heparin, even though both molecules carry the same net anionic charge density. Titration experiments by ITC were conducted in order to further characterize the interaction between HA and LZ.…”

Using affinity capillary electrophoresis and computational models for binding studies of heparinoids with p‐selectin and other proteins

“…In order to determine the binding behavior of P‐selectin and ascertain whether it was a typical or unusual behavior, we have investigated five model proteins, namely human serum albumin (HSA) , bovine serum albumin (BSA) , beta Lactoglobulin (Beta‐LG), myoglobin and ovalbumin , and an additional protein namely vitronectin for their binding behavior with heparinoids. An overview of the main properties of the proteins is summarized in Table .…”

Kinetics and mechanisms of surface-dependent coagulation factor XII activation

“…MAP conceived the study, planned research, analyzed the data, and edited the paper. (Samuel, Pixley et al 1992) (ȝM) A k 307ȝM (Anderot, Nilsson et al 2009) a Values obtained by optimization procedures (for the free-substrate hypothesis, the range is given) b It has been reported that the number fXII molecules to one DS500 chain ranged from 50 to 200 (Samuel, Pixley et al 1992, Citarella, Wuillemin et al 1997) so we decided to include them in fitting procedures to balance the effect of pH variation, because pH is thought to affect the rate of activation by moderation of binding properties (Griep, Fujikawa et al 1985, Griep, Fujikawa et al 1986, Gregory and Basmadjian 1994, Citarella, Wuillemin et al 1997.…”