Preservation of Blood Vessels with an Oxygen Generating Composite

Abstract

Damage caused by oxygen deficiency (hypoxia) is one of the major factors limiting tissue and organ preservation time. Cooling tissues slows down metabolic rate of cells thereby prolonging tissue and organ survival sufficiently to allow transport and transplantation within a few hours. Although metabolism is slowed, cells and some enzymes continue to consume oxygen that can render cold stored tissues hypoxic. Here, an oxygen‐generating composite (OGC) with sustained oxygen release is reported for ex vivo blood vessel preservation. Aorta segments are cultured under hypothermia for 25 days in vascular preservation media. The presence of OGC increases cell viability from 9 ± 6% to 96 ± 3% and retains 65 ± 8% of original KCl stimulated contractile force after 25 days compared with 25 ± 4% in controls. Culture for 7 days in nitrogen demonstrates proof‐of‐concept for normothermic blood vessel preservation, OGC increases the cell viability from 45 ± 15% to 78 ± 2%, and KCl stimulates contractile force from 49 ± 7% to 95 ± 8%, respectively. Oxygen release materials then may have a role in augmenting current preservation techniques.

Oxygen-Releasing Antibacterial Nanofibrous Scaffolds for Tissue Engineering Applications

“…Here, we selected PGS/PCL blend for entrapping CP, which shows a sustainable degradation rate and moderate hydrophilicity. Moreover chloroform-ethanol solvent had no adverse effects on the stability of CP during mixing prior to electrospinning [48].…”

Oxygen-Releasing Biomaterials: Current Challenges and Future Applications

“…Barralet and colleagues used CPO-and MnO 2 -based OGBs to sustain oxygen metabolism during organ preservation time, which is currently limited to several hours only. The study showed an increase in cell survival in aorta explants after 3 weeks with 96 ± 3% of cells surviving when OGB were used compared with 9 ± 6% when conventional vascular preservation media was used [70].…”

3D Printing of Amino Resin-based Photosensitive Materials on Multi-parameter Optimization Design for Vascular Engineering Applications

“…However, it was reported that the wall of the grafts was more prone to pseudoaneurysm and it has little longitudinal elasticity, thus restricting seeding of endothelial cells [6,7]. Therefore, the aim of this study was to develop artificial vascular grafts with structures similar to native blood vessels with the final goal of achieving regenerative medicine and organ reconstruction standards [8,9].…”