Preservation effects of melatonin on the quality and fertility of native Fars rooster semen during liquid storage

Abstract

Liquid or frozen storage of poultry semen negatively affects the sperm motility and has crucial role in reducing fertility. Phospholipids in chicken sperm membrane are composed of high proportions of polyunsaturated fatty acids which are susceptible to lipid peroxidation. One of possible ways to improve semen quality is supplementing the ration with antioxidant compounds such as melatonin. In this study, seventy-two roosters were randomly divided into three equal groups. The first group (control; Group C) was exposed to 14 h light and 10 h darkness. The second group (Group M) was exposed to the same lighting period, but in combination with melatonion supplementation (3 mg/kg body weight daily). The third group (Group L) was exposed to 24 h constant light. Semen was collected using abdominal massage and stored for 6 and 12 h at 5°C. Motility, viability, malondialdehyde (MDA) concentration, fertility, and hatchability were evaluated before and after storage. The results showed that the percentage of viable and motile sperms was significantly (P < 0.05) decreased in the control samples after 6 h storage but in Group M these parameters were significantly (P < 0.05) decreased after 12 h. The MDA concentration was significantly (P < 0.05) decreased in Group M compared with Group C after 6 and 12 h storage. The sperm membrane lipid analysis showed that the percentage of unsaturated fatty acids in Group M was significantly (P < 0.05) lower than in Group C. Fertility and hatchability did not change significantly (P < 0.05) both in M and L Groups compared with C Group. Melatonin administration improved semen quality and decreased lipid peroxidation during liquid storage. It also reduced the percentage of polyunsaturated fatty acids in the sperm membrane lipid composition but it did not affect fertility and hatchability.

Melatonin Alleviated Potassium Dichromate-Induced Oxidative Stress and Reprotoxicity in Male Rats

“…Here, ML alleviated the decrease in sperm count and motility or the increase in sperm abnormalities evoked by PDC. It was previously reported that ML decreased lipid peroxidation in the semen and lowered the ratio of polyunsaturated fatty acids in the sperm membrane lipid composition without affecting fertility [58]. Moreover, ML has the potential to reduce disturbance in spermatogenesis produced by PDC through maintaining testosterone and LH levels that are pivotal for normal spermatogenesis [59].…”

Equilibration and freezing interactions affecting bull sperm characteristics after thawing

“…Therefore, it is necessary to ensure optimal genetic sires selection (Zhang et al 2015) as well as semen processing including semen collection (Dzyuba et al 2015), dilution (Meamar et al 2016), slow cooling to 4-6°C (Kristan et al 2014), and equilibration (Beran et al 2013b) as well as cryopreservation maintaining spermatozoa fertilization capability (Andrabi 2007) evaluated objectively if possible (Simonik et al 2015).…”

Comparison of Commercial Poultry Semen Extenders Modified for Cryopreservation Procedure in the Genetic Resource Program of Czech Golden Spotted Hen

“…The importance of factors such as the semen collection day on CASA and flow cytometric parameters of rooster sperm after thawing was previously reported on by Long et al [ 28 ], Sonseeda et al [ 29 ], Long et al [ 30 ], Meamar et al [ 31 ] and Rakha et al [ 32 ], even though the roosters were kept in the same conditions throughout the experiments. Significant differences among semen collection days were detected for all the evaluated CASA and flow cytometric parameters in our study as well.…”