Comparison of Commercial Poultry Semen Extenders Modified for Cryopreservation Procedure in the Genetic Resource Program of Czech Golden Spotted Hen

Petričáková, Kristýna; Janošíková, Martina; Ptáček, Martin; Zita, Lukáš; Savvulidi, Filipp; Partyka, Agnieszka

doi:10.3390/ani12202886

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…It was reported that the lower abnormality of sperm was produced under a NaCl physiology extender of 4.5-5.5% (Hayanti et al, 2022). It was found in other studies that sperm kept at 4 ⁰C for 72 hours produced abnormality of 8-12% (Petričáková et al, 2022;Zong et al, 2023).…”

Section: Evaluation Of Semenmentioning

confidence: 84%

Semen Evaluation, Preparation of Bangkok Roosters, and Insemination to Indonesian Native Hens

Asnawi,

Maskur,

Dradjat

et al. 2023

JBT

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The success of artificial insemination in chickens is very dependent on sperm quality. Sperm temperature and diluent during storage can affect sperm quality. This study evaluated the quality of Bangkok chicken sperm diluted with 5% glucose and NaCl stored at 5 ⁰C and 26 ⁰C. The research design used was completely randomized. The data obtained were analyzed using a variance. The results showed that the motility of sperm preserved for 9 hours at 26 ⁰C with infused NaCl and Glucose 5% differed significantly (P< 0.05) with motility of 50±0.0% and 34±8.94%, respectively. While preservation under 5 ⁰C for 9 hours with NaCl, better than Glucose 5% (P<0.05) with motility of 58.00±10.95% and 38.00±10.95%, respectively. The viability of sperm preserved at 26 ⁰C with glucose 5% extender was better than NaCl (P<0.05) with a value of 58.93±1.27% and 33.43±1.27%, respectively. While preservation at 5 ⁰C of viabilities of sperm under Glucose 10% and NaCl were not significantly different (P>0.05) from values of 52.57±5.15% and 48.14±8.09%, respectively. The abnormality of sperm stored at 26 ⁰C and 5 ⁰C for 9 hours with NaCl and Glucose 5% were not different (P>0.05). Insemination using 100 million sperm with infused NaCl extender from 18 eggs produced 94.44% (n=17) fertility and 72.22% (n=13) hatchability. While insemination using infuse glucose 5% extender, 11 eggs produced 63.64% (n=7) fertility and 54.54% (n=6) hatchability. The infusing NaCl extender produced better sperm quality, fertility, and hatchability than glucose 5% at 5 ⁰C.

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Section: Evaluation Of Semenmentioning

confidence: 84%

Semen Evaluation, Preparation of Bangkok Roosters, and Insemination to Indonesian Native Hens

Asnawi,

Maskur,

Dradjat

et al. 2023

JBT

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“…PI and YPI dyes are routinely used to determine general sperm viability by flow cytometry or fluorescent microscopy in many species (Garner et al, 1986;Graham et al, 1990;Martin et al, 2004;Ortega-Ferrusola et al, 2008;Trzcińska et al, 2008;Martínez-Pastor et al, 2010;Hossain et al, 2011;García et al, 2012;Anel-López et al, 2017;Hossen et al, 2021;Vašíček et al, 2021;Buchelly Imbachí et al, 2022;Mańkowska et al, 2022;Petričáková et al, 2022;Pytlík et al, 2022) for their ability to penetrate only spermatozoa with destabilized plasma membranes (corrupted plasma membrane or early permeabilization of the plasma membrane). Live sperm cells are not stained with these dyes.…”

Section: Introductionmentioning

confidence: 99%

A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa

Savvulidi,

Ptáček,

Málková

et al. 2023

Fol. Biol.

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Propidium iodide (PI) and YO-PRO-1 (YPI) dyes are routinely used to determine sperm viability in many livestock species. It is commonly accepted that these dyes penetrate only sperm cells with damaged plasma membranes. Recently, however, the mechanism of dye uptake unrelated to damaged plasma membranes, but instead related to pannexin channels in dog and stallion sperm cells was demonstrated. This pilot study aimed to evaluate the role of pannexins in the uptake of PI and YPI dyes on Wallachian frozen-thawed ram spermatozoa by flow cytometry using probenecid, a specific inhibitor of pannexin channels. Additionally, the expression of pannexins in Wallachian sperm was evaluated directly (by qRT-PCR). The results demonstrate the active role of pannexin channels in the uptake of PI and YPI dyes on frozen-thawed Wallachian ram sperm. In conclusion, when using the PI or YPI exclusion assay to determine Wallachian frozen-thawed ram sperm viability, the danger of overestimating the number of spermatozoa with the damaged plasma membrane must be considered. The observed breed-specific, and more importantly, individual differences in gene expression as well as in dye uptake indicate the need for further studies.

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Chicken Sperm Cryopreservation: Review of Techniques, Freezing Damage, and Freezability Mechanisms

Zong

Sun

et al. 2023

Agriculture

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Ex situ preservation is an important method in the preservation of chickens, and cryopreservation of semen is the only method for gamete preservation at present. During the last two decades, many studies have been performed to develop standard chicken semen cryopreservation technology and achieve great progress. Many attempts and methods were investigated to adapt subspecies or different breeds. In this paper, we firstly reviewed the main factors affecting cryopreservation of chicken sperm, including the unique structure and characteristics of the spermatozoa. Secondly, the studies on key points of the chicken sperm cryopreservation technology, including semen dilution, cryoprotectants, equilibration time, packaging types, and freezing and thawing rates were summarized to generate the optimal parameters. Then, the mechanism underlying freezing damage and freezability revealed by recent omics methods relevant to the efficiency of cryopreservation were discussed. This review will provide relevant reference for the future investigation of poultry semen cryopreservation technology.

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Comparison of Commercial Poultry Semen Extenders Modified for Cryopreservation Procedure in the Genetic Resource Program of Czech Golden Spotted Hen

Cited by 4 publications

References 31 publications

Semen Evaluation, Preparation of Bangkok Roosters, and Insemination to Indonesian Native Hens

Semen Evaluation, Preparation of Bangkok Roosters, and Insemination to Indonesian Native Hens

A Pilot Study on the Uptake of Propidium Iodide and YO-PRO-1 Iodide through the Pannexin Channels in Wallachian Frozen-Thawed Ram Spermatozoa

Chicken Sperm Cryopreservation: Review of Techniques, Freezing Damage, and Freezability Mechanisms

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