Presence of resveratrol in wild Arachis species adds new value to this overlooked genetic resource

Abstract

Genus Arachis comprises 82 species distributed into nine taxonomic sections. Most Arachis species are wild and those from Arachis section have been evaluated for many traits, since they can be used in peanut breeding. Most of the remaining species have been neglected and understudied. Recently, resveratrol content and expression of a resveratrol synthase gene were analyzed in wild Arachis species. Our aim was to expand the knowledge about resveratrol in Arachis, analyzing species from five sections and evaluating the expression of a resveratrol synthase (RS) gene responsive to ultraviolet light (UV) along the time. In a first experiment, the resveratrol content after UV induction was analyzed on detached leaves of 12 species from five sections. Variation was observed among species and accessions of the same species. the highest contents were found in A. lignosa (843.9 μg/g) and A. triseminata (745.4 μg/g). In a second experiment, RS expression and resveratrol content in four species and one synthetic amphidiploid were analyzed at 0, 7, 15 and 24 h pos induction (hpi) with UV. In most genotypes, the highest RS expression level was at 0 hpi, whereas the highest resveratrol content was at 15 hpi. Our results suggested that resveratrol is ubiquitously present in the genus Arachis with different capacities of synthesis among species and accessions in response to ultraviolet treatment. presence of resveratrol in wild Arachis species adds new value to these genetic resources.

Transcriptome Responses of Wild Arachis to UV-C Exposure Reveal Genes Involved in General Plant Defense and Priming

“…Although one of the most adopted strategies for stress-induced priming in plants, few studies have addressed the reprogrammed transcriptional responses after UV-C exposure from a transcriptomic point of view [ 8 , 19 , 20 ]. Recently, our group demonstrated that wild Arachis plants exposed to UV-C produce high levels of resveratrol, which is associated with the induced expression of resveratrol synthase genes [ 21 ]. Cultivated peanut ( Arachis hypogaea ) and its wild relatives are among the few plants that naturally synthesize resveratrol, an important phytoalexin selectively accumulated in response to pathogen attack [ 22 ].…”

“…The present study investigated the global transcriptomic changes induced by UV exposure in detached leaves of A. stenosperma to understand better the molecular mechanisms involved in activating plant defense responses to primary stress events and the potential acquisition of a priming state. Detached leaves have long been successfully used as explants for transcriptomic, proteomic, and metabolomic analysis of stress responses induced by UV exposure in leaves of cultivated and wild Arachis species [ 21 , 22 , 30 , 31 ], as well as in several plant species, including grapevine [ 32 , 33 ], soybean [ 34 ], Polygonum cuspidatum [ 35 ], Arabidopsis thaliana [ 36 ], spinach [ 37 ], rice [ 38 ], and maize [ 39 ]. The controlled environment of detached-leaf assays can overcome limitations imposed by field and greenhouse evaluations conducted with whole plants, allowing more standardized assessments and improving the number and homogeneity of samples that could be evaluated.…”

ARACHIS PINTOI KRAPOV. & W.C. GREG. (FABACEAE): A NEW ADDITION TO THE FLORA OF ODISHA

“…Whereas, the concentration of Acid Detergent Fiber (ADF) is widely varies its range from 21% to 41% in dry matter (Hess et al, 2003). This plant has possesses a number of secondary metabolites including sterols, flavonoids, phenolic acids, triterpenes, alkaloids, fatty acid and resveratrol (Carvalho et al, 2020;Tien et al, 2020). Resveratrol is an important bioactive compound having antioxidant properties (Carvalho et al, 2020).…”

“…This plant has possesses a number of secondary metabolites including sterols, flavonoids, phenolic acids, triterpenes, alkaloids, fatty acid and resveratrol (Carvalho et al, 2020;Tien et al, 2020). Resveratrol is an important bioactive compound having antioxidant properties (Carvalho et al, 2020). This bioactive compound (resveratrol) is used as a therapeutic agent for the prevention of treatment of various diseases such as neoplastic, metabolic, cardiovascular, pulmonary as well as neurological disorder (Carvalho et al, 2020;Yang et al, 2016;Meng et al, 2014;Harikumar and Aggarwal, 2008) In India, the species is distributed in forests of Goa, Karnataka, Kerala, Maharashtra, Tamil Nadu, Sikkim and West Bengal.…”

“…Resveratrol is an important bioactive compound having antioxidant properties (Carvalho et al, 2020). This bioactive compound (resveratrol) is used as a therapeutic agent for the prevention of treatment of various diseases such as neoplastic, metabolic, cardiovascular, pulmonary as well as neurological disorder (Carvalho et al, 2020;Yang et al, 2016;Meng et al, 2014;Harikumar and Aggarwal, 2008) In India, the species is distributed in forests of Goa, Karnataka, Kerala, Maharashtra, Tamil Nadu, Sikkim and West Bengal. Study of published literature on flora of Odisha (Haines, 1921-25;Saxena & Brahmam, 1994) and Bhubaneswar (Das and Satapathy, 2020) suggested that the species has not been recorded previously from within the geographical boundary of Odisha, hence it is reported here as a new distributional plant record for the state.…”