Presence of IL 1 Activity in the Supernatant of Human Monocytes Challenged with Purified Allergenic Constituents

Abstract

Purified allergenic constituents from Dermatophagoides farinae mite (Df 11 and Df 6) and from orchard grass (Dactylis glomerata) pollen (F 34) are able to trigger, in the absence of IgE and IgG antibodies, human adherent cells, leading to the secretion of interleukin 1, (IL 1). This secretion was observed with adherent peripheral blood mononuclear cells from normal donors as well as from atopic patients. The IL 1 activity was evaluated by the mice thymocyte mitogenesis in the presence of suboptimal doses of Con A. The secretion of IL 1 by adherent cells was not affected by a cytotoxic treatment with OKT3 monoclonal antibody in the presence of complement, implying a direct action of allergenic fractions, on monocytes, and not through a T-cell pathway. The challenge of human monocytes with allergens in the presence of indomethacin led to culture supernatants for which a higher ³H-thymidine incorporation by mice thymocytes was detected; thus, purified allergens could also trigger the secretion of prostaglandins by human monocytes.

Interleukin-1 Production by Monocytes from Patients with Allergic Asthma after Stimulation in vitro with Lipopolysaccharide and <i>Dermatophagoides pteronyssinus</i> Mite Allergen

“…Monocytes from both patients and controls pro duced IL-1 upon stimulation with LPS or Dp al lergen, in accord with the report of Cavaillon et al [10] that monocytes from 2 atopic patients produced IL-1 after stimulation with purified fractions from Dactylis glomerata and Dermatophagoides farinae. When stimulating monocytes with 1 pg/ml allergen we found that the IL-1 production was of the same magnitude as that by monocytes stimulated with 2 ug/ml LPS.…”

“…Cavaillon et al [10] have reported that various purified allergenic frac tions from the mite Dermatophagoides farinae and the pollen of Dactylis glomerata induce IL-1 production in normal monocytes. Here, we report on the ability of blood monocytes from asthmatics with allergy to the house dust mite Dermatophagoides pteronyssinus (Dp) to produce IL-1 upon stimulation in vitro with the antigen.…”

Interleukin-1 Release by Alveolar Macrophages in Asthmatic Patients and Healthy Subjects

“…This cytokine acts as an important lymphoproliféra tive signal and promotes inflammatory tissue reac tions [8], Peripheral blood monocytes are a major source of IL-1 following stimulation by antigen, al lergen [9] or LPS, a common endotoxin. In contrast, AM from normal human subjects have a limited capacity for synthesizing and releasing IL-1 [10] and exerting a predominant suppressive activity upon lymphocyte proliferation by releasing prostaglandin E2 [11].…”

Quantitative Rather than Qualitative Differences between Monocytes with Respect to IgE Fc Receptor Expression as Studied by Flow Cytofluorometry

“…The expression of FcfR2 on human monocytes is suggested to be a property of a monocyte subpopulation [2], This sub population is believed to have an important role in the pathophysiology of atopic disease, since patients with severe atopic disorders have a significant in crease in FceR2-bearing monocytes [3], and IgE has been shown on macrophages and dendritic cells in the skin lesion of atopic dermatitis [4]. The FcER2 on mon ocytes might be of biological relevance in the allergic inflammatory reaction since complexed IgE induce release of lysosomal enzymes [5], interleukin-1 [6] and induce a metabolic activation [5] with generation of leukotrienes [7], Furthermore, it has been possible to sensitize macrophages from nonaliergic donors with allergen-specific IgE [6].…”