“…However, the active site for PPO and hemocyanin is quite similar (Ling et al, 1994). Increasing evidences for a potential contribution of hemocyanin to the PPO activity have been reported in some crustacean species (Adachi, Hirata, Fujio, Nishioka, & Sakaguchi, 2003a;García-Carreño, Cota, & Navarrete del Toro, 2008;Martínez-Alvarez, Gómez-Guillén, & Montero, 2008a). Hemocyanin could be converted into the PPO enzyme by a yet unknown mechanism, though limited proteolysis, changes in pH and conformational changes induced by some hemolymphs factors have been hypothesised as activating processes (Adachi, Hirata, Nagai, & Sakaguchi, 2001;Decker & Jaenicke, 2004).…”