Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei

Huang, Jianli; Yang, Yang; Wang, An‐Li

doi:10.1016/j.fsi.2009.10.010

Cited by 57 publications

(27 citation statements)

References 22 publications

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“…Serum Phenol peroxidase (PO) activity was analysed according to the method described by (Huang et al . ). All analyses were conducted in triplicate.…”

Section: Methodsmentioning

confidence: 97%

Effects of dietary manganese source and supplemental levels on growth performance, antioxidant enzymes activities, tissue Mn concentrations and cytosolic manganese superoxide dismutase (cMnSOD) mRNA expression level of juvenile Litopenaeus vannamei

et al. 2016

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This experiment was conducted to study the effects of different forms and levels of manganese (Mn) on the growth performance, antioxidant activities, tissue Mn content and cytosolic manganese superoxide dismutase (cMnSOD) gene expression of Litopenaeus vannamei. Treatments consisted of 0, 10, 20, 30, 40 and 60 mg Mn kg−1 from manganese sulphate (Mn‐S) and manganese methionine (Mn‐Met), providing the actual dietary value of 5.17, 15.62, 25.55, 34.22, 44.48 and 67.90 mg Mn kg−1 Mn‐S, and 5.17, 15.71, 25.36, 35.86, 45.16 and 65.06 mg Mn kg−1 Mn‐Met, respectively. Each diet was fed to triplicate groups of L. vannamei (initial body weight: 1.925 ± 0.002 g) in a recirculated fresh water rearing system for 8 weeks. Weight gain rate (WGR) increased in prawns provided with from 25.55 to 44.48 mg Mn kg−1 Mn‐S and 15.71 to 45.16 mg Mn kg−1 Mn‐Met and then declined above these levels. The lowest protein efficiency ratio (PER) and the highest feed conversion rate (FCR) were observed in prawns fed the control diet (P < 0.05) and showed no significant differences among other treatments (P > 0.05). Survival rate (SR) was not affected by the dietary treatments (P > 0.05). Total SOD and Mn‐SOD activities were higher in the hepatopancreas of prawns fed with Mn‐supplemented diets from 15.71 to 44.48 mg Mn kg−1 (P < 0.05). On the contrary, malondialdehyde (MDA) content was lower in the hepatopancreas of prawns fed the basal diet (P < 0.05). Mn concentrations in the hepatopancreas and muscles increased with increasing levels of dietary Mn supplementation. Moreover, Mn accumulation was lower in the muscle than in the hepatopancreas of the prawns. The mRNA expression of cMnSOD gene in the hepatopancreas of prawns was upregulated with increasing dietary Mn levels of Mn‐S from 25.55 to 44.48 mg Mn kg−1, Mn‐Met from 15.71 to 45.16 mg Mn kg−1 and then plateaued above these levels. Broken‐line regression analysis of WGR indicated that the optimal dietary Mn requirements for juvenile L. vannamei were 32.26 mg Mn kg−1 Mn‐S and 23.90 mg Mn kg−1 Mn‐Met, respectively.

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“…Serum Phenol peroxidase (PO) activity was analysed according to the method described by (Huang et al . ). All analyses were conducted in triplicate.…”

Section: Methodsmentioning

confidence: 97%

Effects of dietary manganese source and supplemental levels on growth performance, antioxidant enzymes activities, tissue Mn concentrations and cytosolic manganese superoxide dismutase (cMnSOD) mRNA expression level of juvenile Litopenaeus vannamei

et al. 2016

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“…Total protein content in hepatopancreases was determined using a Coomassie brilliant blue protein assay kit (Nanjing Jiancheng Bioengineering Institute). Polyphenol oxidase (PO) activity was measured spectrophotometrically by recording the formation of dopachrome produced from l ‐di‐hydroxy‐phenylalanine ( l ‐DOPA) following a procedure slightly modified from Huang, Yang and Wang (). In brief, 3 mg mL −1 l ‐DOPA solution was prepared by using 1 L of 0.1 M potassium phosphate buffer (0.1 M K 2 HPO 4 ·3H 2 O, 0.1 M KH 2 PO 4 , adjusted to pH 6.6).…”

Section: Methodsmentioning

confidence: 99%

The effects of a dietary protease‐complex on performance, digestive and immune enzyme activity, and disease resistance of Litopenaeus vannamei fed high plant protein diets

et al. 2016

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The effects of a dietary protease‐complex on growth performance, body composition, digestive and immune enzyme activity of Litopenaeus vannamei and its resistance to a pathogenic Vibrio parahaemolyticus were assessed in a 9‐week trial. A high fish meal diet (HF) containing 200 g kg−1 fish meal and a low fish meal diet (LF) containing 100 g kg−1 fish meal were designed as a positive and negative control respectively. Three other diets (LF+125P, LF+150P, and LF+175P) were manufactured by supplementing graded level of a protease‐complex (125, 150 and 175 mg kg−1, respectively) to the LF diet. All diets were formulated to be iso‐proteic and iso‐energetic. Most performance indices of shrimp fed the LF+175P diets were similar to the HF diet. Among the digestive enzymes, trypsin, lipase and amylase activity in hepatopancreas of shrimp fed LF+175P diets (4576 U mg−1 protein and 16, 32 U g−1 protein, respectively) were higher than those fed the LF diets but lower than the HF diets (P < 0.05). Total superoxide dismutase and polyphenol oxidase contents in both serum and hepatopancreas were higher and serum malondialdehyde content and the cumulative mortality during disease challenge tests were lower for the diets containing the protease‐complex than those fed the LF diets (P < 0.05) with no difference with those fed the HF diets.

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“…Total hemocyte count (THC) was performed on 50 μ L aliquots of 1 : 1 (V/V) dilutions of hemolymph with the anticoagulant described in [22] (27 mM trisodium citrate, 385 mM NaCl, 115 mM glucose, and pH 6.6) and diluted further with formalin (4% and 0.45 M NaCl) to a final 1 : 4 dilution. Counts were performed with a Neubauer chamber at 400X, using the four corner quadrants and an additional one, selected at random [23].…”

Section: Methodsmentioning

confidence: 99%

Humoral and Haemocytic Responses ofLitopenaeus vannameito Cd Exposure

Bautista-Covarrubias

Velarde-Montes

Voltolina³

et al. 2014

The Scientific World Journal

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White shrimp, Litopenaeus vannamei, subadults were exposed to four dilutions of the 96 h cadmium LC50 reported for postlarvae (PL12) of this species, and the effects were evaluated after 5, 48, and 96 h of exposure. While treatments did not affect survival and hemolymph clotting time increased with time, but not as a response to Cd exposure, the intensity of other responses was related to concentration, to time of exposure, and to their interaction. Hemocyanin decreased with time in all metal concentrations but increased in the control treatment, and an almost similar trend was observed with hemocyte numbers. As an initial response, phenoloxidase activity decreased with all metal concentrations, but it increased later to values similar or higher than the control treatment.

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Reconsideration of phenoloxidase activity determination in white shrimp Litopenaeus vannamei

Cited by 57 publications

References 22 publications

Effects of dietary manganese source and supplemental levels on growth performance, antioxidant enzymes activities, tissue Mn concentrations and cytosolic manganese superoxide dismutase (cMnSOD) mRNA expression level of juvenile Litopenaeus vannamei

Effects of dietary manganese source and supplemental levels on growth performance, antioxidant enzymes activities, tissue Mn concentrations and cytosolic manganese superoxide dismutase (cMnSOD) mRNA expression level of juvenile Litopenaeus vannamei

The effects of a dietary protease‐complex on performance, digestive and immune enzyme activity, and disease resistance of Litopenaeus vannamei fed high plant protein diets

Humoral and Haemocytic Responses ofLitopenaeus vannameito Cd Exposure

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