Presence of double‐stranded RNAs and virus‐like particles in the entomopathogenic fungus<i>Metarhizium anisopliae</i>

Leal, Soraya C. M.; Bertioli, David J.; Ball, Brenda V.; Butt, Tariq M.

doi:10.1080/09583159409355316

Cited by 26 publications

(6 citation statements)

References 13 publications

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“…No dsRNA elements were discovered in any of the Danish Metarhizium sp. or B. bassiana isolates, although their presence has been documented previously in populations of Metarhizium mostly from Brazil [32,33,34,35,36,37,38,39]. The number of isolates from other fungal species screened was very small and no dsRNA elements were found in any of these.…”

Section: Resultsmentioning

confidence: 79%

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

Filippou

Garrido‐Jurado

Meyling

et al. 2018

Viruses

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The use of mycoviruses to manipulate the virulence of entomopathogenic fungi employed as biocontrol agents may lead to the development of novel methods to control attacks by insect pests. Such approaches are urgently required, as existing agrochemicals are being withdrawn from the market due to environmental and health concerns. The aim of this work is to investigate the presence and diversity of mycoviruses in large panels of entomopathogenic fungi, mostly from Spain and Denmark. In total, 151 isolates belonging to the genera Beauveria, Metarhizium, Lecanicillium, Purpureocillium, Isaria, and Paecilomyces were screened for the presence of dsRNA elements and 12 Spanish B. bassiana isolates were found to harbor mycoviruses. All identified mycoviruses belong to three previously characterised species, the officially recognised Beauveria bassiana victorivirus 1 (BbVV-1) and the proposed Beauveria bassiana partitivirus 2 (BbPV-2) and Beauveria bassiana polymycovirus 1 (BbPmV-1); individual B. bassiana isolates may harbor up to three of these mycoviruses. Notably, these mycovirus species are under distinct selection pressures, while recombination of viral genomes increases population diversity. Phylogenetic analysis of the RNA-dependent RNA polymerase gene sequences revealed that the current population structure in Spain is potentially a result of both vertical and horizontal mycovirus transmission. Finally, pathogenicity experiments using the Mediterranean fruit fly Ceratitis capitata showed no direct correlation between the presence of any particular mycovirus and the virulence of the B. bassiana isolates, but illustrated potentially interesting isolates that exhibit relatively high virulence, which will be used in more detailed virulence experimentation in the future.

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Section: Resultsmentioning

confidence: 79%

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

Filippou

Garrido‐Jurado

Meyling

et al. 2018

Viruses

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“…Studies exploring sources of genetic variation among these asexual fungi reported the presence of double-stranded RNA (Castrillo et al, 2004;Leal et al, 1994;Sugimoto et al, 2003), transposons (Maurer et al, 1997b), and chromosomal polymorphism (Viaud et al, 1996). Genetic change via the parasexual cycle has also been investigated by examining vegetative compatibility groups and the potential for asexual recombination (Cantone and Vandenberg, 1998;Castrillo et al, 2004;Paccola-Meirelles and Azevedo, 1991).…”

Section: Systematics and Evolutionmentioning

confidence: 98%

The fungal past, present, and future: Germination, ramification, and reproduction

Castrillo

Roberts

Vandenberg

2005

Journal of Invertebrate Pathology

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“…Numerous mycoviruses have been identi ed from various entomopathogenic ascomycete fungi that can kill or induce disease in insects or other arthropods, including Acari (ticks and mites) [2,5,39], such as Beauveria bassiana [13,17,26,47], Tolypocladium cylindrosporum [16], Metarhizium spp. [29,41,42], Cordyceps chanhua [49], Purpureocillium lilacinum [18] and Isaria javanica [19]. Further identi cation of mycoviruses from arthropod-associated fungi would expand our knowledge of the diversity of mycoviruses in this fungal group and facilitate studies to understand the effect of mycovirus infection on the phenotypes and pathogenicity of entomopathogenic fungi.…”

Section: Full Textmentioning

confidence: 99%

Identification of a novel member of the genus Laulavirus (family Phenuiviridae) from the entomopathogenic ascomycete fungus Cordyceps javanica

Andika,

Cao,

Liu

et al. 2024

Preprint

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The virus family Phenuiviridae (order Bunyavirales, comprising segmented negative-sense single stranded RNA viruses) has highly diverse members that are known to infect animals, plants, protozoans and fungi. In this study, we identified a novel phenuivirus infecting a strain of the entomopathogenic fungus Cordyceps javanica, tentatively named Cordyceps javanica negative-strand RNA virus 1 (CjNRSV1). The CjNRSV1 genome consisted of three negative-sense single stranded RNA segments (RNA1–3) with lengths of 7252, 2401 and 1117 nt, respectively. The 3¢- and 5¢-terminal regions of the RNA1, 2, and 3 segments had identical sequences, and the termini of the RNA segments were complementary to each other, reflecting a common characteristic of viruses in the order Bunyavirales. RNA1 encoded a large protein (~274 kDa) containing a conserved domain for the bunyavirus RNA-dependent RNA polymerase (RdRP) superfamily, with 57–80% identity to the RdRP encoded by phenuiviruses in the genus Laulavirus. RNA2 encoded a protein (~79 kDa) with no conserved domain but showing identity (47–63%) to the movement protein (MP, a plant viral cell-to-cell movement protein)-like protein (MP-L) encoded by RNA2 of laulaviruses. RNA3 encoded a protein (~28 kDa) with a conserved domain for the phenuivirid nucleocapsid protein superfamily. Moreover, in phylogenetic analysis using the RdRPs of various phenuiviruses and other unclassified phenuiviruses, CjNRSV1 was grouped with established members of the genus Laulavirus. Our results suggest that CjNRSV1 is a novel fungus-infecting member of the genus Laulavirus in the family Phenuiviridae.

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Presence of double‐stranded RNAs and virus‐like particles in the entomopathogenic fungusMetarhizium anisopliae

Cited by 26 publications

References 13 publications

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

Mycoviral Population Dynamics in Spanish Isolates of the Entomopathogenic Fungus Beauveria bassiana

The fungal past, present, and future: Germination, ramification, and reproduction

Identification of a novel member of the genus Laulavirus (family Phenuiviridae) from the entomopathogenic ascomycete fungus Cordyceps javanica

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