Preparative chromatography and natural products discovery

McChesney, James D.; Rodenburg, Douglas L.

doi:10.1016/j.copbio.2013.11.002

Cited by 8 publications

(13 citation statements)

References 2 publications

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“…However, the time range for peak collection associated with the higher hydrophobicity of AnAc monoene caused a decrease in its recovery. The concentration loading of 200 mg resulted in low percentage recovery for AcAn triene and monoene due to column saturation , thus showing chromatographic peaks with wider base and coelution.…”

Section: Resultsmentioning

confidence: 99%

Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid

Filho

Zocolo

Canuto

et al. 2019

Separation Science Plus

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The anacardic acids are alkyl phenols, with biological activities, obtained as a byproduct from cashew nut processing. The anacardic acids isolation by preparative reversed phase high-performance liquid chromatography was developed injecting large pulse from 50 to 200 mg of cashew nut shell liquid through a C 18 column eluted with methanol, water and acetic acid (80:20:1). The separations were carried out at 25 • C and monitored at 280 nm. Yield, recovery, purity, solvent consumption and productivity were analyzed. The results of anacardic acids isolation pointed the concentration loading of 100 mg as a process threshold leading to a productivity of 0.681 Kg.day −1 .Kg −1 of anacardic acids with purity of 96.41, 95.32 and 90.77% for anacardic acid triene, diene and monoene, respectively. K E Y W O R D Salkyl phenols, Anacardium occidentale, concentration overloading, ginkgolic acid, preparative chromatography, purification 192

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Section: Resultsmentioning

confidence: 99%

Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid

Filho

Zocolo

Canuto

et al. 2019

Separation Science Plus

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“…9 For example, during the separation process of isosteviol from a mixture of steviol and other isomers, the optimal retention factors were observed with n-heptane:waEtOAc (8:2 v/v) whereas for the optimum separation the rate of MTBE was increased in the mixture n-heptane: waMTBE (7:3 v/v).…”

Section: Mobile Phase Amentioning

confidence: 99%

“…The solubility of water in EtOAc is approximately 3.3% v/v at room temperature. 7 However, the detection of the steviol glycosides is generally set at 210 nm, below the UV absorbance cut off wavelength of ethyl acetate. An alternative mobile phase to solve this problem is the use of methyl tert-butyl ether (MTBE) instead of ethyl acetate.…”

Section: Mobile Phase Amentioning

confidence: 99%

“…More details regarding the improvement of the performance of the columns packed with silica gel stationary phase and their use in multiple column runs have been well summarized by McChesney. 7,9 Mobile phases…”

Section: Stationary Phasesmentioning

confidence: 99%

“…7 In the purification processes using a semi-preparative and preparative columns (2.5 × 45 cm; 10 µm), solvent was pumped with two Shimadzu pumps (LC-10AS) connected in series to produce a maximum flow rate of 20 mL min -1 . The larger preparative columns (7.5 × 50 cm; 10 µm, spherical) were eluted at a flow rate of 200 mL min -1 using a Prep 250 pump (Scientific Systems, Inc., State College, Pennsylvania).…”

Section: Preparative Separationsmentioning

confidence: 99%

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Development of HPLC Analytical Techniques for Diterpene Glycosides fromStevia rebaudiana(Bertoni) Bertoni: Strategies to Scale-Up

Rodenburg¹,

Alves²,

Perera³

et al. 2016

Journal of the Brazilian Chemical Society

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Stevia rebaudiana is a plant well-known as a source of dozens of high potency non-caloric natural sweeteners, many of them still uncharacterized. Herein, we describe the development of several routine HPLC (high performance liquid chromatography) analytical techniques to better separate steviol glycosides. Different stationary phases/columns were used: reverse phase C-18, amino, HILIC (hydrophilic interaction chromatography), Sepaxdiol and Synergi. The advantages and disadvantages of each method for resolving the very polar and less polar elution regions are demonstrated. Strategies for scale up of the purification process of steviol glycosides to make it more economic are also summarized, emphasizing the advantages of using high efficiency normal phase chromatography in relation with reverse phase. Additionally, we also developed an easy and useful way for predicting yields and composition of the pools resulting from the preparative chromatography. The development of these analytical and preparative liquid chromatography techniques allowed the isolation of several steviol glycosides in gram quantities at relatively low cost.

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Version updating of strategy for drug discovery based on effective constituents of traditional Chinese medicines

Sun

et al. 2023

Acupuncture and Herbal Medicine

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The discovery of effective constituents of traditional Chinese medicine (TCM) is an important approach in new drug development. Several well-known drugs, such as artemisinin, berberine, and taxol have been developed using this approach. However, the efficacy and safety of TCM, two key issues for drug development based on TCM clinical experience, remain unclear worldwide. The discovery of relevant constituents is the most important step for determining efficacy and safety. However, TCM formulas used as clinical drugs address a specific TCM syndrome (Zheng), and the complexity of the formula and vagueness of the syndrome make the identification of the effective constituents related to clinical effectiveness challenging. Over decades, researchers have developed transdisciplinary technologies and research methodologies to identify effective constituents in vivo. In this paper, the history of strategy development for identifying the effective constituents related to the clinical efficacy of TCM is reviewed and summarized. The main approaches include the phytochemical method, which involves the classical systematic separation and screening (extraction, separation, purification, structure identification, and activity test); bioactivity-guided separation; serum pharmacochemistry of TCM in vivo; and Chinmedomics, which connects in vivo constituents with the biomarkers of the relevant TCM syndrome. Chinmedomics is a promising strategy to help elucidate the material requirements for the efficacy of TCM, advance the discovery of lead compounds and innovative drugs, and promote modernization in TCM. http://links.lww.com/AHM/A64

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Preparative chromatography and natural products discovery

Cited by 8 publications

References 2 publications

Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid

Productivity of a preparative high‐performance liquid chromatography isolation of anacardic acids from cashew nut shell liquid

Development of HPLC Analytical Techniques for Diterpene Glycosides fromStevia rebaudiana(Bertoni) Bertoni: Strategies to Scale-Up

Version updating of strategy for drug discovery based on effective constituents of traditional Chinese medicines

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