Preparative chromatographic separation: Simulated moving bed and modified chromatography methods

Effect of pH on fractional precipitation for pre-purification of paclitaxel from plant cell cultures

“…The low-resolution chromatography steps require such large amounts of solvent and chromatographic media that the process is impractical for scale-up to commercial operations [15][16][17].…”

Optimal temperature control in fractional precipitation for paclitaxel pre-purification

“…The resulting crude paclitaxel products typically contain between 1 and 4% paclitaxel by weight and the final separation of paclitaxel is accomplished using open column chromatography, HPLC, or a combination of the two methods. The low-resolution chromatography steps require such large amounts of solvent and chromatographic media that the process is impractical for scale-up to commercial operations [20][21][22]. Fig.…”

Advanced Operating Strategies to Extend the Applications of Simulated Moving Bed Chromatography

“…The main features of the SMB process, its numerous applications, and its theoretical description have been recently summarized in a book [18] and several reviews [16,[19][20][21][22][23][24]. The concept of adsorption in processes ranging from simple batch chromatography to the SMB has been well described along with an introduction of their practical application to the petrochemical and food industries [2].…”

“…In the main part of the review, several selected new variants of the SMB technology, which can significantly expand its potential, are described. This review is not intended as a comprehensive compilation of all options and variants proposed in recent years, as many successful applications have already been reviewed [16,[19][20][21][22][23][24] and more detailed information regarding modeling and controlling SMB can be obtained in the literature [24][25][26][27][28][29]. Accordingly, this review simply introduces and describes the advances, with a focus on introducing novel concepts and providing examples and advantages of recently proposed operating strategies in the following specific SMB areas: manipulations during shifts, altering elution strength, and separation of ternary mixtures (center cut separation).…”