Optimal temperature control in fractional precipitation for paclitaxel pre-purification

Jeon, Sun Im; Mun, Sungyong; Kim, Jin-Hyun

doi:10.1016/j.procbio.2005.07.016

Cited by 21 publications

(2 citation statements)

References 14 publications

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“…The yield was usually low and with a problem of mass solvent waste. To solve this problem, we developed a fractional precipitation approach based on Jeon's study . Three factors (methanol concentration, material‐to‐solvent ratio, and precipitating time) were under consideration using response surface methodology (RSM) for condition optimization in this study, while the previous one tested only the temperature.…”

Section: Introductionmentioning

confidence: 99%

“…To solve this problem, we developed a fractional precipitation approach based on Jeon's study . Three factors (methanol concentration, material‐to‐solvent ratio, and precipitating time) were under consideration using response surface methodology (RSM) for condition optimization in this study, while the previous one tested only the temperature. Besides, it was revealed that the yield and purity of paclitaxel in this study surpassed that of the Jeon's study .…”

Section: Introductionmentioning

confidence: 99%

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Optimization of the fractional precipitation of paclitaxel from a Taxus chinensis cell culture using response surface methodology and its isolation by consecutive high‐speed countercurrent chromatography

Liang

Xie

Lam

et al. 2014

J of Separation Science

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A consecutive preparation method for the isolation and purification of paclitaxel from the Taxus Chinensis cell culture was developed in this study. The process involved alkaline Al2O3 chromatography, fractional precipitation, and high-speed countercurrent chromatography. The original cell culture materials were first extracted with methanol using ultrasound-assisted extraction, and then the extract (the content of paclitaxel is 1.5%) was separated by alkaline Al2O3 column chromatography. Subsequently, fractional precipitation was used to obtain paclitaxel. In particular, response surface methodology was used to optimize the factors of fractional precipitation (methanol concentration, material-to-solvent ratio, and precipitating time were optimized as 48.14%, 8.85 mg/mL, and 48.71 h, respectively) and the yield of fractional precipitation product was 30.64 ± 0.60 mg (the content of paclitaxel is 89.3%, 27.37 ± 0.54 mg) from a 100 mg fraction by Al2O3 column separation (the content of paclitaxel is 32.4%). Then, the product was used for further isolation by high-speed countercurrent chromatography. About 1.00 g paclitaxel (200 ± 2 mg in each loading) with a purity up to 99.61% was isolated from 1.25 g of fractional precipitation product with a solvent system of n-hexane/ethyl acetate/methanol/water (1.2:1.8:1.5:1.5, v/v/v/v) in one run of five consecutive sample loadings without exchanging a new solvent system.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%