Preparation of novel bio-matrix by the complexation of DNA and metal ions

Yamada, Masashi; Yokota, Michio; Kaya, Michiko; Satoh, Shunji; Jonganurakkun, Benjamas; Nomizu, Motoyoshi; Nishi, Norio

doi:10.1016/j.polymer.2005.07.086

Cited by 38 publications

(27 citation statements)

References 37 publications

(51 reference statements)

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“…In general, metal ions such as Cu(II), Co(II), Mn(II) and Mg(II) bind to ds-DNA at both phosphate groups and base residues sites, and Al(III) binds to it at the former site. [36][37][38][39] Al(III) binding to ds-DNA at base residues sites was previously proposed by Karlik et al, 21 and testified to be the case by 27 Al NMR. 24 Our studies have shown that about 53.5% of Al(III) is probably binding to ds-DNA at phosphate groups sites, and the other 46.5% of Al(III) is kinetically united at base residues sites.…”

Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 83%

“…21,36 However, the absorption strengths at 245.6 and 274.2 nm gradually increased with an increase of Al(III). The increment of the CD optical absorption of phosphorylated neurofilament with 17 amino acid sequences in the presence of Ca(II) was assigned to an increase in the production of an unordered structure component.…”

Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 99%

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Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

et al. 2009

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The interaction between double-strand calf thymus gland DNA (ds-DNA) and Al(III) was studied by using differential pulse voltammetry (DPV) at a hanging mercury drop electrode (HMDE), Raman spectrometry and circular dichroism (CD) spectra. It was shown that at neutral pH ds-DNA did not produce any cathodic peak at the HMDE in the potential window from -550 to -2000 mV vs. SCE. However, in the presence of Al(III), a cathodic peak was generated at about -1660 mV, which is ascribed to a reduction of adenine and cytosine residues of single denatured DNA (sd-DNA). It was concluded that ds-DNA was completely denatured to sd-DNA by Al(III) at a neutral pH. The apparent denaturing kinetic velocity constants of ds-DNA by Al(III) were derived from linear increases of the cathodic peak currents with time.3 ≥ 2 × 10 -26 , the precipitation of Al(OH)3 was observed and identified by the Raman spectrum, and inductively coupled plasma atomic emission spectrometry (ICP-AES). CD spectra showed that the B-type of structure conformations of ds-DNA and related sd-DNA did not change with the increment of Al(III) from 5.0 × 10 -7 to 1.0 10 -5 M, but the corresponding absorption strengths increased. The related physiological significances and possible applications of the observations were considered.

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Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 83%

Section: Al(oh)3 Precipitation Analyzed By Icp-aes and Raman Spectramentioning

confidence: 99%

Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

et al. 2009

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“…[7][8][9] We could also convert the discarded DNA into a novel bio-matrix by the hybridization of DNA and metal ions. 10 These DNA-metal ion matrices could be created in various desirable forms, such as a gel, capsule, film, or fiber. 10 Additionally, DNA-metal ion biomatrix indicated the oxidative property.…”

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“…10 These DNA-metal ion matrices could be created in various desirable forms, such as a gel, capsule, film, or fiber. 10 Additionally, DNA-metal ion biomatrix indicated the oxidative property. 10 Metal ions have many functions, such as reductive effect, catalytic action, or the change of coordination number.…”

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Utilization of DNA-metal Ion Biomatrix as a Relative Humidity Sensor

Yamada

Sugiyama

2008

Polym J

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Recently, we prepared the novel biomatrix by the mixing of DNA and metal ion. These biomatrices have a metal ion property, such as oxidative and reducible effects, catalytic action, or the change of coordination number. So, we proposed the humidity sensor for the novel utilization of DNA-metal ion biomatrix. The biomatrix with the humidity property was prepared by the mixing of DNA and metal ion, such as cobalt (II) or nickel (II) ions. These matrices indicated the different color with the change of relative humidity (RH). Especially, the color of Co 2þ -containing biomatrix changed from blue to red with the increase of RH. These biomatrices may have a potential for the novel RH sensor with flexibility, low cost, nonhazardous, and environmentally benign.KEY WORDS: DNA / Biopolymers / Matrix / Humidity Sensor / Composites / DNA, one of the most important materials for the genetic process, can easily be purified from either salmon milts or shellfish gonads, which are generally discarded as industrial waste around the world. In addition, double-stranded DNA has highly specific functions, 1,2 such as the accumulation of intercalating compounds, the complementary interaction between nucleic acid bases, and the interaction with metal ions. Therefore, the double stranded DNA has been attracted for novel functional materials, such as adsorbents of harmful compounds, 3,4 electro-and ion-conductive materials, 5,6 and functional materials. [7][8][9] We could also convert the discarded DNA into a novel bio-matrix by the hybridization of DNA and metal ions.10 These DNA-metal ion matrices could be created in various desirable forms, such as a gel, capsule, film, or fiber.10 Additionally, DNA-metal ion biomatrix indicated the oxidative property.10 Metal ions have many functions, such as reductive effect, catalytic action, or the change of coordination number. Therefore, DNA-metal ion biomatrices might be used for various functional materials.Cobalt (II) and nickel (II) ions can indicate the different color by the change of coordination number with water molecules 11,12 and this property has been used for the silica gel with the moisture indicator. However, this material is rigid and non-flexible, as a result, the utilized-fields for the moisture indicator or humidity sensor have been limited. So, we propose the relative humidity (RH) sensor for the novel utilization of DNA-metal ion biomatrix that is flexible, non-hazardous, biodegradable, and environmentally benign. These RH sensors with the DNA are novel engineering material and have not been reported as far as we know.In this paper, we prepared DNA-metal ion biomatrix by the mixing of double-stranded DNA and metal ion, such as cobalt (II) and nickel (II) ions. This matrix had been constructed by the electrostatic interaction between the phosphate group of DNA and metal ion. This DNA-metal ion biomatrix indicated the color change with the RH. Additionally, these color changes is quickly and reversible. Furthermore, the similar phenomena were also obtained at the biomatrix co...

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“…[33][34][35] Bio-inspired materials or natural biopolymers have attracted much attention for a industrial applications as a biocompatible material. [36][37][38][39][40][41][42][43][44][45][46] Although these materials are often discarded as industrial waste around the world, there is an increasing interest towards the application of the biomaterials in devices because they have well-controlled structures and superior conducting properties. The controlled hydrogen-bonded network in biomolecules, such as bioenergetic proteins, is essential for efficient energy transduction in living systems, 47,48 and these biomolecules may be efficient electrolytes with superior conducting properties.…”

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confidence: 99%

Bio-Inspired Membranes for Advanced Polymer Electrolyte Fuel Cells. Anhydrous Proton-Conducting Membrane via Molecular Self-Assembly

Honma¹,

Yamada²

2007

Bulletin of the Chemical Society of Japan

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Bio-inspired materials or natural biopolymers have attracted much attention for industrial applications as biocompatible materials. Although these materials have been discarded as industrial waste around the world, there is an increasing interest in environmental, engineering, and electrical applications of the biomaterials because of their well-controlled structures and superior conducting properties. The controlled structures of the hydrogen-bonding network in biomolecules, such as bioenergetic proteins, are essential for efficient energy transduction in living systems and application of a biomolecular mechanism could make it possible to prepare efficient electrolytes with superior conducting properties. We report here a recent investigation on the new class and new concept of electrolyte materials with bio-inspired molecular architectures of acid-base pairs for anhydrous proton conduction and its application in intermediate temperature C) polymer electrolyte fuel cells (PEFC). Although some membranes were composed from entirely biomolecular materials, they showed large anhydrous proton conductivities from room temperature to 160 C, and fuel cells employing biomembranes as a polymer electrolyte produced electrical power under non-humidified H 2 /O 2 condition at 160 C. These bio-inspired materials may have many potential applications not only because of its superior ion conducting properties, in particular, under anhydrous (water-free) or extremely low-humidity conditions, but also because of its biocompatibility.Increasing interest toward the global sustainable energy has occurred in relation to political, economical, and environmental protection aspects, whereas, from an industrial development point of view, advanced energy technology is becoming intensively important recently in ordered to reduce fossil fuel consumption and keep the environment clean for human activities. The Kyoto protocol has stimulated research activities in renewable energy technology in every nation and every industrial sector. Among them, polymer electrolyte fuel cells (PEFC) have been recognized as most promising energy conversion devices because they are much very efficient and emit less of CO 2 from automobile, terrestrial, and industrial power sources.

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Preparation of novel bio-matrix by the complexation of DNA and metal ions

Cited by 38 publications

References 37 publications

Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

Electrochemical and Spectrometric Studies of Double-Strand Calf Thymus Gland DNA Denatured by Al(III) at Neutral pH

Utilization of DNA-metal Ion Biomatrix as a Relative Humidity Sensor

Bio-Inspired Membranes for Advanced Polymer Electrolyte Fuel Cells. Anhydrous Proton-Conducting Membrane via Molecular Self-Assembly

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