Preparation of Nanofibers with Renewable Polymers and Their Application in Wound Dressing

Zhao, Ying Na; Qiu, Yue; Wang, Huanhuan; Chen, Yu; Jin, Shaohua; Chen, Shuseng

doi:10.1155/2016/4672839

Cited by 67 publications

(38 citation statements)

References 114 publications

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“…Nanofibers prepared from renewable polymers, due to their extremely high specific surface area, porosity and excellent pore interconnectivity, excellent performances in cell adhesion, migration, proliferation, differentiation, and the analogous physical properties of the extracellular matrix (ECM) have attracted considerable attention over the past two decades 4 . Such characteristics allow structured nanofibers to promote hemostasis without the use of a hemostatic agent, maintain an adequately humid environment for the wound by facilitating oxygen permeation and allowing fluid accumulation, protect the wound effectively against bacterial penetration, and be easily functionalized with therapeutic compounds 5 .…”

mentioning

confidence: 99%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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To eliminate the microbial infection from an injury site, various modalities have been developed such as dressings and human skin substitutes. However, the high amount of reactive oxygen species, microbial infection, and damaging extracellular matrix remain as the main challenges for the wound healing process. In this study, for the first time, green synthesized silver nanoparticles (AgNPs) using Teucrium polium extract were embedded in poly lactic acid/poly ethylene glycol (PLA/PEG) film to provide absorbable wound dressing, with antioxidant and antibacterial features. The physicochemical analysis demonstrated, production of AgNPs with size approximately 32.2 nm and confirmed the presence of phytoconstituents on their surface. The antibacterial assessments exhibited a concentration-dependent sensitivity of Staphylococcus aureus and Pseudomonas aeruginosa toward biosynthesized AgNPs, which showed a suitable safety profile in human macrophage cells. Furthermore, oxidant scavenging assays demonstrated exploitation of plant extract as a reducing agent, endows antioxidant activity to biogenic AgNPs. The formation of PLA/PEG nanofilm and entrapment of AgNPs into their matrix were clearly confirmed by scanning electron microscopy. More importantly, antibacterial examination demonstrated that the introduction of biogenic AgNPs into PLA/PEG nanofibers led to complete growth inhibition of P. aeruginosa and S. aureus. In summary, the simultaneous antioxidant activity and antimicrobial activity of the novel biogenic AgNPs/PLA/PEG nanofilm showed its potential for application as wound dressing.activities of porous Ag nanofilm were investigated. We anticipate that the Ag nanofilm wound dressing with free radical scavenging capacity would enhance the wound healing process. Moreover, this study proposed a low-cost and facile method for preparation of Ag nanofilm as a potential wound dressing. Materials and MethodsMaterials. Methanol (CH 3 OH, 99.9%), silver nitrate (AgNO 3 ), nutrient agar, ferric chloride hexahydrate (FeCl 3 , 6H 2 O), iron (II) sulfate tetrahydrate (FeSO 4 .4H 2 O), dichloromethane, polyethylene glycol 300, ascorbic acid and Mueller-Hinton agar (MHA) were purchased from Merck (Germany). 2,2-diphenyl-1-picrylhydrazyl (DPPH), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and 2,4,6-Tripyridyl-s-Triazine (TPTZ) were purchased from Sigma (Germany) and poly lactic acid (PLA) from Zhejiang Hisun Biomaterials (China). The RAW264 macrophage cell line was purchased from National Cell Bank, Pasteur Institute of Iran, Iran and Dulbecco's Modified Eagle's Medium (DMEM), Fetal Bovine Serum (FBS), 1% Penicillin-Streptomycin from Gibco (Paisley, UK). All aqueous solutions were prepared using double distilled water. All reagents used were of analytical grade. Clinical strains of Pseudomonas aeruginosa 38 and Staphylococcus aureus 39 bacteria were isolated from hospitalized patients by our group, which have been characterized as antibiotic-resistant strains.Extract preparation. T.polium is a wild-growing...

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confidence: 99%

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Bardania

Mahmoudi

Bagheri

et al. 2020

Sci Rep

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“…Commonly used methods for the fabrication of twoand three-dimensional scaffolds are freeze-drying, phase separation, salt leaching, cell encapsulation, gas forming, and electrospinning, [11][12][13][14][15] as well as bubbfil and centrifugal spinning. 16 Likewise, textile technologies are a suitable approach for a fiber-based scaffold fabrication, considering that with these technologies structural, topographical, and mechanical properties of the constructs are widely adjustable. The most common techniques applied for textile scaffold preparation are weaving, knitting, braiding, nonwoven technologies, and electrospinning.…”

mentioning

confidence: 99%

Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

Tonndorf

Gossla

Kocaman

et al. 2017

Textile Research Journal

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The field of articular cartilage tissue engineering has developed rapidly, and chitosan has become a promising material for scaffold fabrication. For this paper, wet-spun biocompatible chitosan filament yarns were converted into short flock fibers and subsequently electrostatically flocked onto a chitosan substrate, resulting in a pure, highly open, porous, and biodegradable chitosan scaffold. Analyzing the wet-spinning of chitosan revealed its advantages and disadvantages with respect to the fabrication of the fiber-based chitosan scaffolds. The scaffolds were prepared using varying processing parameters and were analyzed in regards to their geometrical and mechanical properties. It was found that the pore sizes were adjustable between 65 and 310 µm, and the compressive strength was in the range 13–57 kPa.

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“…5,18 The further benefits of a handheld portable electrospinning device for the purposes of wound care are beginning to emerge and reported within the peer-reviewed literature. [19][20][21][22][23][24] The development of biocompatible electrospun nanofibrous dressings speaks to the versatility, simplicity, adaptability and importantly cost effectiveness.…”

Section: Discussionmentioning

confidence: 99%

“…2 Electrospinning technology is a method for the creation and utilization of nanofibers and has received much attention in peer-reviewed literature. [4][5][6][7][8] Electrospun nanofibers dressings have in the past received positive attention as an ideal dressing due to their unique architectural features that mimic the extracellular matrix and indeed provide an ideal wound environment for wound healing. 9,10 Nanofibers provide a large surface area-to-volume ratio, are highly permeable, and have small pore size suitable for biomedical applications such as wound healing.…”

Section: Introductionmentioning

confidence: 99%

The Feasibility of a Handheld Electrospinning Device for the Application of Nanofibrous Wound Dressings

Haik

Kornhaber

Blal

et al. 2017

Advances in Wound Care

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{These authors contributed equally to this work. Objectives:The aim of this study was to determine the feasibility of a portable electrospinning device for the application of wound dressings. Approach: Four polymer nanofibers dressings were applied on superficial partial thickness wounds to a porcine model and compared with a traditional paraffin tulle gras dressing. The polymer nanofibrous dressings were applied using a handheld portable electrospinning device activated at a short distance from the wound. The partial thickness donor sites were evaluated on day 2, 7, and 14 when dressings were removed and tissue samples were taken for histological examination. Results: No significant difference was detected between the different electrospun nanofibrous dressings and traditional paraffin tulle gras. Desirable characteristics of the electrospun nanofiber dressing group included nontouch technique, ease of application, adherence and reduction in wound edema and inflammation. There was no delayed wound healing or signs of infection reported in both the electrospun nanofiber and traditional tulle gras dressings. Innovation: Used on partial thickness wounds, polymer electrospun nanofiber dressings provide excellent surface topography and are a nontouch, feasible, and safe method to promote wound healing with the potential to reduce wound infections. Such custom-made nanofibrous dressings have implications for the reduction of pain and trauma, number of dressing changes, scarring, and an added cost benefit. Conclusion: We have demonstrated that this portable handheld electrospinning device can be utilized for different formulations and materials and customized according to the characteristics of the target wound at the various stages of wound healing.

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Preparation of Nanofibers with Renewable Polymers and Their Application in Wound Dressing

Cited by 67 publications

References 114 publications

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Facile preparation of a novel biogenic silver-loaded Nanofilm with intrinsic anti-bacterial and oxidant scavenging activities for wound healing

Factors affecting the mechanical and geometrical properties of electrostatically flocked pure chitosan fiber scaffolds

The Feasibility of a Handheld Electrospinning Device for the Application of Nanofibrous Wound Dressings

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