Preparation of Leaf Epidermis for Topographic Study

Clarke, J.A.

doi:10.3109/10520296009114713

Cited by 76 publications

(32 citation statements)

References 7 publications

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“…The first pair of fully expanded leaves was used. Methods of peeling of the epidermis were based on those of Clarke (1960) and Speckman et al (1965). A strip of abaxial epidermis from the middle portion of the leaf was peeled off and mounted in glycerol after staining with safranin.…”

Section: Study Of Stomatamentioning

confidence: 99%

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2011

Plant Cell Tiss Organ Cult

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To induce variation through chromosome doubling in Gerbera jamesonii Bolus cv. Sciella, twoweek-old in vitro grown shoots were treated with various concentrations of colchicine (0.01, 0.05, 0.10, 0.50 or 1% w/v) for 2, 4 or 8 h. Treated shoots were then cultured on Murashige and Skoog (MS) medium supplemented with 8.8 lM 6-benzyladenine (BA) and 155 lM adenine sulphate (ADS), and subsequently transferred to fresh MS medium containing 2.85 lM indole-3 acetic acid (IAA) for rooting. When shoots were treated with 0.1% colchicine for 8 h, 64% of recovered plantlets were tetraploid. Ploidy of plantlets was confirmed by flow cytometry, stomatal analysis, and morphological characters. Tetraploid plantlets displayed slower proliferation along with higher vigor and thickened broad leaves. Moreover, tetraploid plants developed larger flowers, longer stalks, and have improved vase-life, all contributing to higher ornamental value of gerbera.

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Section: Study Of Stomatamentioning

confidence: 99%

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

Gantait

Mandal

Bhattacharyya

et al. 2011

Plant Cell Tiss Organ Cult

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“…Starting from a threshold value, to be derived from diploid standard material, it will be possible to recognize the tetraploid plant parts in C o material or to distinguish 2 …”

Section: Discussionmentioning

confidence: 99%

The length of stomata as an indicator for polyploidy in rye-grasses

1965

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“…They were prepared according to the modified method of Clark (1960). The fresh parts were placed in a tube filled with 88% lactic acid, kept hot in boiling water bath for about 50-60 minutes.…”

Section: Histologymentioning

confidence: 99%

Pharmacognostic Studies on Two Himalayan Species of Traditional Medicinal Value: <i>Allium wallichii</i> and <i>Allium stracheyi<i/>

TIWARI¹,

Adams

S³

et al. 2014

Not Sci Biol

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Abstract AbstractThe present research was aimed as a pharmacognostic study of whole plants of Allium wallichii and Allium stracheyi, both of which are very important traditional medicinal plants of North-West Himalayas. This study was carried out in terms of macroscopic and microscopic analyses and standard histochemical methods were followed for detecting starch, calcium oxalate, tannins, total lipids and alkaloids. Allium wallichi can be distinguished from A. stracheyi by possessing polyarch roots, mycorrhizal fungi in the outer cortical cells and triangular leaf midrib. The present study is the first to describe the pharmacognosy in terms of anatomical and histochemical features of these two Himalayan Allium species. Although they are listed in Ayurvedic database, the API so far has not given an account on these two species and hence this work is of high importance. Also, the herbal industries, researchers and traditional medicine can now use the distinguishing characters of these species listed in the current paper, while specifically acquiring them from local markets without any confusion.

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Preparation of Leaf Epidermis for Topographic Study

Cited by 76 publications

References 7 publications

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella

The length of stomata as an indicator for polyploidy in rye-grasses

Pharmacognostic Studies on Two Himalayan Species of Traditional Medicinal Value: <i>Allium wallichii</i> and <i>Allium stracheyi<i/>

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