Preparation of lactoperoxidase incorporated hybrid nanoflower and its excellent activity and stability

A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

“…Inspiring from Zare and coworkers, several research groups studied the preparation and characterization of organic-inorganic hybrid nanoflowers using different enzymes and metal ions for different purposes [42][43][44][45][46][47][48][49]. In one of our study, we determined how the experimental parameters, such as concentrations of copper (Cu 2+ ) ions, chloride ions (Cl -) and HRP enzyme; the pH of the buffer solution (phosphate buffered saline); and the temperature of the reaction, affect the morphology and activity performance of synthesized nanoflowers.…”

Self-assembled organic⿿inorganic hybrid glucoamylase nanoflowers with enhanced activity and stability

“…At pH 7.5, nanoflowers were formed with less packed structure due to slightly increased negative charge on enzyme molecules whereas; glucoamylase nanoflowers were unable to form at or above pH 9.5. It was due to strong repulsions between high density negative charge on the enzyme surface, which repel Cu3(PO4)2 •3H2O petals rather than forcing them to bind with each other [30].…”

“…Also, hybrid glucoamylase nanoflowers showed 2.38, 2.21 and 1.90 folds increased half-life with respect to free glucoamylase at 50, 60 and 70°C respectively. Enhanced thermal stability indicates that glucoamylase nanoflowers need more time to denature by heat as compared to their free form [28][29][30][31][32][33][34][35].…”

Formation of functional nanobiocatalysts with a novel and encouraging immobilization approach and their versatile bioanalytical applications

“…In terms of the inorganic component, some metal ions including Cu 2+ , Fe 2+ and Ca 2+ have been used as the inorganic part of FNFs. 37-47 A wide range of enzymes [37][38][39][40][41][42][43][44][45][46][47][48][49][50] including commercially available and isolated enzymes such as HRP, 37-39 SBP, 40 alfa-chymotrypsin, 41 laccase, 42,47 urease, 43 lactoperoxidase, 44 lipase, 45 Turkish black radish peroxidase, 46 and trypsin 47 have been successfully incorporated into FNF synthesis. FNFs have provided a variety of applications such as colorimetric sensors to detect H 2 O 2 , phenol, 37 dopamine, 38,44 epinephrine, 42,44 dye decolorization, 46,50 and protein digestion.…”