A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

Altınkaynak, Cevahir; Tavlasoglu, Sureyya; Özdemir, Nalan; Öçsoy, İsmail

doi:10.1016/j.enzmictec.2016.06.011

Cited by 206 publications

(114 citation statements)

References 54 publications

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“…Ge et al . reported an innovative strategy to synthesize hybrid nanostructures having flower‐like shapes (nanoflowers) based on the combination of enzyme molecules with metal salts in phosphate buffer ,. The hybrid nanoflowers were more active and stable than conventionally immobilized enzymes owing to their large surface area and eﬀective confinement of enzyme molecules in their core.…”

Section: Introductionmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

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Herein we report an expeditious and facile sonochemical synthesis method that takes just a few minutes to produce organic‐inorganic hybrid nanoflowers and their application as a nanobiocatalyst. Surfactant treated Burkholderia cepacia lipase was used as the organic component and manganese sulfate as the inorganic component to synthesize the nanoflowers in less than 10 minutes. Careful investigation of the process parameters was performed to obtain the optimized value for each attribute viz. sonication time (7.5 min), lipase concentration (0.1 mg/mL), MnSO4 concentration (150 mM), sonication frequency (33 kHz) and pH (8.0). An additional post‐synthesis treatment of nanoflowers with N‐hydroxysuccinimide (NHS) increased their reusability to over nine cycles with an overall six‐fold increase in the activity. In addition, thermal stability was demonstrated through a twenty‐fold increase in the half‐life of immobilized lipase at 80 °C than in free form. The hybrid nanoflowers displayed a marked improvement in the kinetic resolution of racemic intermediates of metoprolol, a selective β‐1 blocker drug [C (%)=46.7, eep=96.5, E=156] and cloranolol, a non‐selective β‐blocker drug [C (%)=49.5, eep=96.0, E=157] compared to free lipase which signifies its importance as a nanobiocatalyst.

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Section: Introductionmentioning

confidence: 99%

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

2018

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“…reported that a hybrid nanoflower with organic component of Burkholderia cepacia lipase and inorganic component of calcium phosphate with enhanced activity of 308% of the original one . Altinkaynak and coworkers have introduced an elegant immobilization approach for the synthesis of flower‐like organic–inorganic hybrid nanostructures with extraordinary catalytic activity and stability . Wang et al .…”

Section: Introductionmentioning

confidence: 80%

“…19 Altinkaynak and coworkers have introduced an elegant immobilization approach for the synthesis of flower-like organic-inorganic hybrid nanostructures with extraordinary catalytic activity and stability. 20 Wang et al 21 also reported the rational design of a CaHPO 4 --amylase hybrid nanobiocatalytic system based on the allosteric effect. The increase in catalytic activity when enzymes are confined in a specific compartmentalization was explained as allosteric modulation and hierarchical structure.…”

Section: Introductionmentioning

confidence: 99%

Efficient synthesis of the key chiral alcohol intermediate of Crizotinib using dual‐enzyme@CaHPO₄ hybrid nanoflowers assembled by mimetic biomineralization

Chen

Wang

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND To develop an environment‐friendly approach for the synthesis of (S)‐1‐(2,6‐dichloro‐3‐fluorophenyl) ethyl alcohol, an intermediate of anti‐cancer drug Crizotinib, aldehyde ketone reductase and alcohol dehydrogenase were overexpressed in Escherichia coli Rosetta (DE3) and purified via chromatography. Subsequently, they were co‐crystalized with CaHPO4 at 4°C to form dual‐enzyme@CaHPO4 hybrid nanoflowers (hNFs) which was then used to catalyze the synthesis of (S)‐1‐(2,6‐dichloro‐3‐fluorophenyl) ethyl alcohol along with the evaluation of its thermal stability and recycling stability. RESULTS At optimum pH of 7.0, the activities of AKR and ADH confined in the dual‐enzyme@CaHPO4 hybrid nanoflowers were 3.3‐ and 2.1‐fold that of the corresponding free one. The thermos‐stability of confined enzymes was also significantly improved: both enzymes within the hNFs remained more than 80% of initial activities after incubation at 60°C for 8 h, while free enzymes only retained 20% of initial activities under the same treatment conditions. Moreover, AKR and ADH immobilized with a mole ratio of 3:1 confined in hybrid nanoflowers exhibited the highest catalytic activity for the synthesis of chiral ethyl alcohol with a yield up to 90.8% after 12 h. Besides, the final product (S)‐1‐(2,6‐dichloro‐3‐fluorophenyl) ethyl alcohol showed a high ee value of 99.99%. Further, the hybrid nanoflowers retained their initial activity after 16 recycling cycles of synthesis reaction. CONCLUSION The dual‐enzyme@CaHPO4 hybrid nanoflowers efficiently catalyzed synthesis of the chiral compound (S)‐1‐(2,6‐dichloro‐3‐fluorophenyl) ethyl alcohol. The method can also be applied to other multi‐enzyme systems and facilitate their cascade reactions and substrate channeling. © 2018 Society of Chemical Industry

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“…However, the disadvantages of free enzymes, including high cost, poor operational stability and challenges in recovery and reuse, have limited industrial applications of enzymes [7]. To solve these issues, immobilization techniques are considered, because binding of free enzymes to supports limits their mobility [8]. What is more, some immobilized enzymes could show more robust activity than free enzymes [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

In-Situ Self-Assembly of Zinc/Adenine Hybrid Nanomaterials for Enzyme Immobilization

et al. 2017

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Abstract:In this study, a one-step and facile immobilization of enzymes by self-assembly of zinc ions and adenine in aqueous solution with mild conditions was reported. Enzymes, such as glucose oxidase (GOx) and horseradish peroxidase (HRP), could be efficiently encapsulated in Zn/adenine coordination polymers (CPs) with high loading capacity over 90%. When the enzyme was immobilized by CPs, it displayed high catalytic efficiency, high selectivity and enhanced stability due to the protecting effect of the rigid framework. As a result, the relative activity of Zn/adenine nano-CP-immobilized GOx increased by 1.5-fold at pH 3 and 4-fold at 70 to 90 • C, compared to free GOx. The immobilized GOx had excellent reusability (more than 90% relative activity after being reused eight times). Furthermore, the use of this system as a glucose biosensor was also demonstrated by co-immobilization of two enzymes, detecting glucose down to 1.84 µM with excellent selectivity. The above work indicated that in-situ self-assembly of Zn/adenine CPs could be a simple and efficient method for biocatalyst immobilization.

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A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

Cited by 206 publications

References 54 publications

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

Efficient synthesis of the key chiral alcohol intermediate of Crizotinib using dual‐enzyme@CaHPO₄ hybrid nanoflowers assembled by mimetic biomineralization

In-Situ Self-Assembly of Zinc/Adenine Hybrid Nanomaterials for Enzyme Immobilization

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A new generation approach in enzyme immobilization: Organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability

Cited by 206 publications

References 54 publications

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

An Ultrafast Sonochemical Strategy to Synthesize Lipase‐Manganese Phosphate Hybrid Nanoflowers with Promoted Biocatalytic Performance in the Kinetic Resolution of β‐Aryloxyalcohols

Efficient synthesis of the key chiral alcohol intermediate of Crizotinib using dual‐enzyme@CaHPO4 hybrid nanoflowers assembled by mimetic biomineralization

In-Situ Self-Assembly of Zinc/Adenine Hybrid Nanomaterials for Enzyme Immobilization

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Efficient synthesis of the key chiral alcohol intermediate of Crizotinib using dual‐enzyme@CaHPO₄ hybrid nanoflowers assembled by mimetic biomineralization