Self-assembled organic⿿inorganic hybrid glucoamylase nanoflowers with enhanced activity and stability

Nadar, Shamraja S.; Gawas, Sarita D.; Rathod, Virendra K.

doi:10.1016/j.ijbiomac.2016.06.071

Cited by 103 publications

(62 citation statements)

References 39 publications

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“…The aqueous CuSO 4 solution was added to phosphate buffered saline (PBS) containing enzyme. 212 The hybrid nanoflowers of this work showed 204% enhanced activity recovery and two folds improvement in thermal stability in terms of half-life (in the range of 50-70 °C) with respect to the free form. Besides that, it showed higher storage stability with retention of 91% activity after 25 days of incubation.…”

Section: Organic -Inorganic Hybrid Nanoflowermentioning

confidence: 79%

“…Since then, the attention in synthesizing protein molecule-metal phosphate hybrid materials has been majorly increased. 210,211 As demonstrated by Nadar et al, 212 the copper phosphate was used to prepare an organic-inorganic hybrid glucoamylase nanoflower. The aqueous CuSO 4 solution was added to phosphate buffered saline (PBS) containing enzyme.…”

Section: Organic -Inorganic Hybrid Nanoflowermentioning

confidence: 99%

“…Besides that, it showed higher storage stability with retention of 91% activity after 25 days of incubation. 212 Copper phosphate also was used to synthesize lactoperoxidase (LPO). 213 Altinkaynak and coworkers developed a hybrid nanoflowers (HNF) formed of LPO enzyme purified from bovine milk and copper ions (Cu 2+ ) were synthesized at two different temperatures (+4 °C and 20 °C) in PBS (pH 7.4).…”

Section: Organic -Inorganic Hybrid Nanoflowermentioning

confidence: 99%

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Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Reis

Sousa

Serpa

et al. 2019

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The enzymatic processes are increasingly highlights, especially in the synthesis of chemical products with high added value. The enzyme immobilization can improve industrial biocatalytic processes. The immobilization of enzymes provides the production of efficient, stable biocatalysts, possibility of reuse and easy purification of the products, when compared to the free enzymes. There is a growing research for more efficient methods of enzyme immobilization. In this context, the choice of support and immobilization strategy can significantly improve the final enzymatic properties. In this review paper, we aimed to discuss the versatility of biocatalysts immobilized enzymes design, focusing on the opportunities and disadvantages for each method presented. They discussed the recent development of enzyme immobilization methods and applications relating the final properties of the produced biocatalysts with the desired goals.

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Section: Organic -Inorganic Hybrid Nanoflowermentioning

confidence: 79%

Section: Organic -Inorganic Hybrid Nanoflowermentioning

confidence: 99%

Section: Organic -Inorganic Hybrid Nanoflowermentioning

confidence: 99%

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Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Reis

Sousa

Serpa

et al. 2019

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“…The as‐obtained hybrid materials showed enhanced enzymatic activity as well as improved stability and reusability. With the extensive studies, bovine serum albumin, papain, glucoamylase, laccase, peroxidase, and metalloporphyrins were used as organic components, while copper phosphate, manganese phosphate, cobaltous phosphate, zinc phosphate, and calcium phosphate were employed as inorganic components . And it should be noted that in many previous reports, the synergistic effects between organic and inorganic components were also observed.…”

Section: Figurementioning

confidence: 99%

Self‐Assembled Metalloporphyrins–Magnesium Phosphate Hybrid Spheres as Efficient Catalysts for Cycloaddition of Carbon Dioxide

et al. 2019

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Due to the unique microstructure and superior catalytic properties, organic-inorganic hybrid materials attract extensive attention. Herein, we report a facile coprecipitation method for the fabrication of metalloporphyrin-metal phosphate salt hybrid spheres, and the cycloaddition of carbon dioxide with epoxides is employed as probe reaction to evaluate the catalytic performance of our as-synthesized catalysts. The hybrid spheres composed of meso-tetra-(4-N-pyridyl) zinc porphyrin (ZnTPyP)magnesium phosphate exhibited superior catalytic performance, and the conversion was much higher than that of individual ZnTPyP and magnesium phosphate, probably because of the acid-base synergistic effect. Meanwhile, the spheres showed excellent stability during the circulation experiments as well as compatibility with a wide scope of substrates. This work provides a valuable approach for the preparation of novel and efficient heterogeneous catalytic composites.Organic-inorganic hybrid materials with hierarchical structures have attracted ever increasing attention due to their potential applications related to catalysis, adsorption, drug delivery, and energy storage. [1] Recently, an encouraging breakthrough in the synthesis of organic-inorganic hybrid materials derived from biomolecules emerged. Ge et al. reported a convenient and ecofriendly coprecipitation method to synthesize hybrid nanoflowers based on the combination of enzyme molecules with metal salts in phosphate buffer. [2] The as-obtained hybrid materials showed enhanced enzymatic activity as well as improved stability and reusability. With the extensive studies, bovine serum albumin, [3] papain, [4] glucoamylase, [5] laccase, [6] peroxidase, [7] and metalloporphyrins [8] were used as organic components, while copper phosphate, [9] manganese phosphate, [10] cobaltous phosphate, [3] zinc phosphate, [11] and calcium phosphate were employed as inorganic components. [12] And it should be noted that in many previous reports, the synergistic effects between organic and inorganic components were also observed. Comparing with the ordinary catalytic systems of individual components, the hybrid materials composed of organic and inorganic components showed remarkable enhancement in the catalytic activities. For example, the enzyme activity of the trypsin embedded nanoflowers, which was reported by Lin et al., was determined approximately 270% higher than free trypsin in solution. [13] The results showed that the dramatically enhanced activity of trypsin embedded hybrid nanoflowers can be attributed to the allosteric modulation. In addition, as reported by Wang et al.,

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“…21 Majority studies have demonstrated that enzymeinorganic hNFs showed highly enhanced activity, stability and selectivity in comparison with those of free enzyme. [22][23][24][25][26][27] Also, enzyme-inorganic hNFs can be easily synthesized with very fast speed and do not require any toxic elements or very harsh conditions. 28 Therefore, enzyme-inorganic hNFs are widely studied for the application in bioremediation, biosensing, enzymatic biofuel cells, bioanalytical devices, pharmaceutical applications and industrial biocatalysis.…”

Section: Introductionmentioning

confidence: 99%