Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell–matrix interactions

Jiang, Yongchao; Wang, Haonan; Wang, Xiaofeng; Yu, Xueke; Li, Haojie; Tang, Keyong; Li, Qian

doi:10.1177/08853282211018567

Cited by 9 publications

(5 citation statements)

References 35 publications

(37 reference statements)

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“…Likewise, as shown in , respectively, which were ~60, ~10, and ~4 times higher than that of the pristine PVA membrane. These results are consistent with those of previous studies showing that the Hofmeister effect has profound effects on improving natural or synthetic hydrophilic polymer-based scaffolds [22][23][24][25]. In addition, the mechanical properties of all hybrid porous membranes were much better than those of the composite PVA membranes.…”

Section: Mechanical Properties Of Polyvinyl Alcohol Pp and Citrate-tr...supporting

confidence: 92%

Preparation and characterisation of tough and porous polyvinyl alcohol/POC membrane for biomedical applications

Niu

You

et al. 2022

Biosurface and Biotribology

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Polyvinyl alcohol (PVA) is a synthetic polymer that has been extensively studied for fabricating porous membranes via electrospinning for diverse biomedical applications. However, the poor mechanical properties of electrostatically spun PVA nanofiber membranes severely limit their application in the biomedical field. Therefore, porous, tough hybrid PVA‐based fibrous membranes were prepared by introducing poly (1,8‐octanediol citrate) (POC) into PVA fibrous membranes followed by sodium citrate treatment. The tensile modulus, fracture strength, and fracture toughness of the sodium citrate‐treated PVA/POC (CPP) membranes achieve 119.81 ± 5.32 MPa, 10.34 ± 1.57 MPa and 401.51 ± 11.67 MJ m−2, respectively, which were ∼60, ∼10, and ∼4 times higher than those achieved by the pristine PVA membrane. Moreover, the novel CPP membranes exhibited suitable biodegradation ratios and high cell/issue affinities, suggesting their potential biomedical applications in soft or hard tissue repair. This strategy, which provides porous structures, high mechanical properties and excellent biocompatibility, demonstrates a facile but effective approach for the development of advanced biomaterials.

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Section: Mechanical Properties Of Polyvinyl Alcohol Pp and Citrate-tr...supporting

confidence: 92%

Preparation and characterisation of tough and porous polyvinyl alcohol/POC membrane for biomedical applications

Niu

You

et al. 2022

Biosurface and Biotribology

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“…The mechanical properties of the native extracellular matrix (ECM) play a key role in regulating cell behavior during development, healing, and homeostasis. With respect to the biomaterial mechanical signals, it has been demonstrated that manipulating them can promote changes in cell behaviors such as spreading, proliferation, migration, and differentiation [ 50 , 51 ]. Therefore, mimicking the brain ECM microenvironment by engineering hydrogels capable of coping with some mechanical stress from adjacent tissues and maintaining at least a slight elasticity and bendability without collapse or losing their shape may allow for investigation and better understanding of NSC behavior.…”

Section: Resultsmentioning

confidence: 99%

Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation

et al. 2022

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Tissue engineering scaffolds provide biological and physiochemical cures to guide tissue recovery, and electrical signals through the electroactive materials possess tremendous potential to modulate the cell fate. In this study, a novel electroactive hydrogel scaffold was fabricated by assembling poly(3,4-ethylenedioxythiophene) (PEDOT) nanoparticles on a carboxymethyl chitosan/gelatin (CMCS/Gel) composite hydrogel surface via in situ chemical polymerization. The chemical structure, morphology, conductivity, porosity, swelling rate, in vitro biodegradation, and mechanical properties of the prepared hydrogel samples were characterized. The adhesion, proliferation, and differentiation of neural stem cells (NSCs) on conductive hydrogels were investigated. The CMCS/Gel-PEDOT hydrogels exhibited high porosity, excellent water absorption, improved thermal stability, and adequate biodegradability. Importantly, the mechanical properties of the prepared hydrogels were similar to those of brain tissue, with electrical conductivity up to (1.52 ± 0.15) × 10−3 S/cm. Compared to the CMCS/Gel hydrogel, the incorporation of PEDOT nanoparticles significantly improved the adhesion of NSCs, and supported long-term cell growth and proliferation in a three-dimensional (3D) microenvironment. In addition, under the differentiation condition, the conductive hydrogel also significantly enhanced neuronal differentiation with the up-regulation of β-tubulin III expression. These results suggest that CMCS/Gel-PEDOT hydrogels may be an attractive conductive substrate for further studies on neural tissue repair and regeneration.

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“…Nevertheless, these hydrogels lack the required strength. Consequently, the development of a novel injectable hydrogel with excellent cell proliferation and high strength is crucial for applications of meniscus tissue engineering [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Development of Biphasic Injectable Hydrogels for Meniscus Scaffold from Photocrosslinked Glycidyl Methacrylate-Modified Poly(Vinyl Alcohol)/Glycidyl Methacrylate-Modified Silk Fibroin

Jeencham,

Sinna,

Ruksakulpiwat

et al. 2024

Polymers

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The development of a hydrogel material with a modified chemical structure of poly(vinyl alcohol) (PVA) and silk fibroin (SF) using glycidyl methacrylate (GMA) (denoted as PVA-g-GMA and SF-g-GMA) is an innovative approach in the field of biomaterials and meniscus tissue engineering in this study. The PVA-g-GMA/SF-g-GMA hydrogel was fabricated using different ratios of PVA-g-GMA to SF-g-GMA: 100/0, 75/25, 50/50, 25/75, and 0/100 (w/w of dry substances), using lithium phenyl (2,4,6-trimethylbenzoyl)phosphinate (LAP) as a free radical photoinitiator, for 10 min at a low ultraviolet (UV) intensity (365 nm, 6 mW/cm2). The mechanical properties, morphology, pore size, and biodegradability of the PVA-g-GMA/SF-g-GMA hydrogel were investigated. Finally, for clinical application, human chondrocyte cell lines (HCPCs) were mixed into PVA-g-GMA/SF-g-GMA solutions and fabricated into hydrogel to study the viability of live and dead cells and gene expression. The results indicate that as the SF-g-GMA content increased, the compressive modulus of the PVA-g-GMA/SF-g-GMA hydrogel dropped from approximately 173 to 11 kPa. The degradation rates of PVA-g-GMA/SF-g-GMA 100/0, 75/25, and 50/50 reached up to 15.61%, 17.23%, and 18.93% in 4 months, respectively. In all PVA-g-GMA/SF-g-GMA conditions on day 7, chondrocyte cell vitality exceeded 80%. The PVA-g-GMA/SF-g-GMA 75:25 and 50:50 hydrogels hold promise as a biomimetic biphasic injectable hydrogel for encapsulated augmentation, offering advantages in terms of rapid photocurability, tunable mechanical properties, favorable biological responses, and controlled degradation.

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Preparation of gelatin-based hydrogels with tunable mechanical properties and modulation on cell–matrix interactions

Cited by 9 publications

References 35 publications

Preparation and characterisation of tough and porous polyvinyl alcohol/POC membrane for biomedical applications

Preparation and characterisation of tough and porous polyvinyl alcohol/POC membrane for biomedical applications

Carboxymethyl Chitosan and Gelatin Hydrogel Scaffolds Incorporated with Conductive PEDOT Nanoparticles for Improved Neural Stem Cell Proliferation and Neuronal Differentiation

Development of Biphasic Injectable Hydrogels for Meniscus Scaffold from Photocrosslinked Glycidyl Methacrylate-Modified Poly(Vinyl Alcohol)/Glycidyl Methacrylate-Modified Silk Fibroin

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