Preparation of ds-DNA functionalized magnetic nanobaits for screening of bioactive compounds from medicinal plant

Li, Yuan; Xu, Pei-Li; Zeng, Qiong; Liu, Yiming; Ding, Li‐Sheng; Liao, Xun

doi:10.1016/j.msec.2015.06.050

Cited by 11 publications

(7 citation statements)

References 51 publications

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“…In the cyclic voltammetry (CV), the immobilization of nano‐composite on electrodes usually results in a change of current response; therefore, CV has usually been used to characterize the nano‐composite . In this work, the cyclic voltammograms of mCNTs and PD‐mCNTs were measured in phosphate buffer (PBS) (pH 7) containing 0.1 M KCl and 5 mM potassium ferricyanide.…”

Section: Resultsmentioning

confidence: 99%

“…Supporting electrolyte: pH 7 PBS containing 5 mM FeCN(K 3 Fe(CN) 6 ) and 0.1 M KCl; scan rate: 50 mV/s. Carbon rings served as the working and counter electrodes, Ag/AgCl reference electrode current response; therefore, CV has usually been used to characterize the nano-composite [40,41]. In this work, the cyclic voltammograms of mCNTs and PD-mCNTs were measured in phosphate buffer (PBS) (pH 7) containing 0.1 M KCl and 5 mM potassium ferricyanide.…”

Section: F I G U R E 3 (A) the Image Of Pd-mcnts (Left) And Mcnts (Rimentioning

confidence: 99%

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Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Wan

Zhang

Bai

et al. 2017

J of Separation Science

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A new, rapid, green, and cost-effective magnetic solid-phase extraction of ochratoxin A from red wine samples was developed using polydopamine-coated magnetic multi-walled carbon nanotubes as the absorbent. The polydopamine-coated magnetic multi-walled carbon nanotubes were fabricated with magnetic multi-walled carbon nanotubes and dopamine by an in situ oxidative self-polymerization approach. Transmission electron microscopy, dynamic light scattering, X-ray photoelectron spectroscopy and vibrating sample magnetometry were used to characterize the absorbents. Ochratoxin A was quantified with high-performance liquid chromatography coupled with fluorescence detection, with excitation and emission wavelengths of 338 and 455 nm, respectively. The conditions affecting the magnetic solid-phase extraction procedure, such as pH, extraction solution, extraction time, absorbent amount, desorption solution and desorption time were investigated to obtain the optimal extraction conditions. Under the optimized conditions, the extraction recovery was 91.8-104.5% for ochratoxin A. A linear calibration curve was obtained in the range of 0.1-2.0 ng/mL. The limit of detection was 0.07 ng/mL, and the limit of quantitation was 0.21 ng/mL. The recoveries of ochratoxin A for spiked red wine sample ranged from 95.65 to 100.65% with relative standard deviation less than 8%. The polydopamine-coated magnetic multi-walled carbon nanotubes showed a high affinity toward ochratoxin A, allowing selective extraction and quantification of ochratoxin A from complex sample matrixes.

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Section: Resultsmentioning

confidence: 99%

Section: F I G U R E 3 (A) the Image Of Pd-mcnts (Left) And Mcnts (Rimentioning

confidence: 99%

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Wan

Zhang

Bai

et al. 2017

J of Separation Science

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“…FTIR was used to characterize the surface modification of the MNPs. The electrochemical method could be used for CHO-MNPs and AG-MNPs analysis in which immobilization of the MNPs on the electrode usually resulted in the change of current response [ 26 ]. In this work, by using carbon as a working/counter electrode and Ag/AgCl as a reference electrode for the electrochemical workstation, the cyclic voltammograms (CV) and the differential pulse voltammograms (DPV) of the nano-composites were measured in PBS (10 mM, pH 7.0) containing 5 mM K 3 [Fe(CN) 6 ] and 0.1 M KCl.…”

Section: Methodsmentioning

confidence: 99%

Fishing of α-Glucosidase’s Ligands from Aloe vera by α-Glucosidase Functionalized Magnetic Nanoparticles

Yuan

Wan

et al. 2021

Molecules

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α-Glucosidase was immobilized on magnetic nanoparticles (MNPs) for selective solid-phase extraction of the enzyme’s ligands present in Aloe vera, which is a medicinal plant used for the treatment of various diseases and possesses anti-diabetic activity. One new compound, aloeacone (2), together with two known compounds, aloenin aglycone (1) and aloin A (3), were fished out as the enzyme’s ligands. The structure of 2 was determined by HR-MS and comprehensive NMR techniques. Compound 3 exhibited a weak inhibitory effect on α-glucosidase, while compounds 1 and 2 were found to possess activation effects on the enzyme for the first time. It is interesting that both an inhibitor and agonists of α-glucosidase were fished out in one experiment.

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“…Based on our survey, eight works used DNA strands (double, triple, and quadruple) as smart baits to fish out ligands from natural products with anticancer potential. Different approaches were used to apply DNA strands into ligand fishing settings, including dialysis [186], UF [12], MPs [187], and microfluid chips [188,189] as well as other less trivial supports such as streptavidin-coated 96-well plates [49,50] and agarose beads [47]. The DNA-bound ligands were tested in preclinical settings in two specific reports.…”

Section: Monoacylglycerol Lipasementioning

confidence: 99%

Ligand fishing as a tool to screen natural products with anticancer potential

Filho

Oliveira

Leal

et al. 2023

J of Separation Science

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Cancer is the second leading cause of death in the world and its incidence is expected to increase with the aging of the world's population and globalization of risk factors. Natural products and their derivatives have provided a significant number of approved anticancer drugs and the development of robust and selective screening assays for the identification of lead anticancer natural products are essential in the challenge of developing personalized targeted therapies tailored to the genetic and molecular characteristics of tumors. To this end, a ligand fishing assay is a remarkable tool to rapidly and rigorously screen complex matrices, such as plant extracts, for the isolation and identification of specific ligands that bind to relevant pharmacological targets. In this paper, we review the application of ligand fishing with cancer‐related targets to screen natural product extracts for the isolation and identification of selective ligands. We provide critical analysis of the system configurations, targets, and key phytochemical classes related to the field of anticancer research. Based on the data collected, ligand fishing emerges as a robust and powerful screening system for the rapid discovery of new anticancer drugs from natural resources. It is currently an underexplored strategy according to its considerable potential.

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Preparation of ds-DNA functionalized magnetic nanobaits for screening of bioactive compounds from medicinal plant

Cited by 11 publications

References 51 publications

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Fishing of α-Glucosidase’s Ligands from Aloe vera by α-Glucosidase Functionalized Magnetic Nanoparticles

Ligand fishing as a tool to screen natural products with anticancer potential

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