Extraction of ochratoxin A in red wine with dopamine‐coated magnetic multi‐walled carbon nanotubes

Abstract

A new, rapid, green, and cost-effective magnetic solid-phase extraction of ochratoxin A from red wine samples was developed using polydopamine-coated magnetic multi-walled carbon nanotubes as the absorbent. The polydopamine-coated magnetic multi-walled carbon nanotubes were fabricated with magnetic multi-walled carbon nanotubes and dopamine by an in situ oxidative self-polymerization approach. Transmission electron microscopy, dynamic light scattering, X-ray photoelectron spectroscopy and vibrating sample magnetometry were used to characterize the absorbents. Ochratoxin A was quantified with high-performance liquid chromatography coupled with fluorescence detection, with excitation and emission wavelengths of 338 and 455 nm, respectively. The conditions affecting the magnetic solid-phase extraction procedure, such as pH, extraction solution, extraction time, absorbent amount, desorption solution and desorption time were investigated to obtain the optimal extraction conditions. Under the optimized conditions, the extraction recovery was 91.8-104.5% for ochratoxin A. A linear calibration curve was obtained in the range of 0.1-2.0 ng/mL. The limit of detection was 0.07 ng/mL, and the limit of quantitation was 0.21 ng/mL. The recoveries of ochratoxin A for spiked red wine sample ranged from 95.65 to 100.65% with relative standard deviation less than 8%. The polydopamine-coated magnetic multi-walled carbon nanotubes showed a high affinity toward ochratoxin A, allowing selective extraction and quantification of ochratoxin A from complex sample matrixes.

Recent advances on emerging nanomaterials for controlling the mycotoxin contamination: From detection to elimination

“…Nanomaterials, especially magnetic nanoparticles (MNP), have been successfully integrated with various analysis systems (e.g., HPLC and LC—MS/MS) for the extraction and detection of contaminants in foods, such as polycyclic aromatic hydrocarbons (Li et al., 2018), fluoroquinolones (Wen et al., 2020), mycotoxins (Yang et al., 2020), and etc. (Liu et al., 2020; Moreno, Zougagh, & Ríos, 2016; Ramadan, Mohamed, Almoammar, & Abd‐Elsalam, 2020; Wan et al., 2017; Yang et al., 2020; Zhao, Wang, Xu, & Lu, 2017). By employing Fe 3 O 4 MNPs as a cleanup material for the extraction of the mycotoxin (DON), Karami‐Osboo et al.…”

“…Nanomaterials, especially magnetic nanoparticles (MNP), have been successfully integrated with various analysis systems (e.g., HPLC and LC-MS/MS) for the extraction and detection of contaminants in foods, such as polycyclic aromatic hydrocarbons , fluoroquinolones (Wen et al, 2020), mycotoxins , and etc. Moreno, Zougagh, & Ríos, 2016;Ramadan, Mohamed, Almoammar, & Abd-Elsalam, 2020;Wan et al, 2017;Zhao, Wang, Xu, & Lu, 2017 (Yu, Ma, Zhang, & Li, 2019) have been also applied for the HPLC-based assays of mycotoxins (Capriotti et al, 2019). Although HPLC-based assays have showed high sensitivity, good accuracy, and robust reliability for the mycotoxin detection, they are laborious, time consuming, and complicated, and especially relying on the expensive equipment with skilled operators (Goud, Reddy, Satyanarayana, Kummari, & Gobi, 2019;.…”