Fishing of α-Glucosidase’s Ligands from Aloe vera by α-Glucosidase Functionalized Magnetic Nanoparticles

Abstract

α-Glucosidase was immobilized on magnetic nanoparticles (MNPs) for selective solid-phase extraction of the enzyme’s ligands present in Aloe vera, which is a medicinal plant used for the treatment of various diseases and possesses anti-diabetic activity. One new compound, aloeacone (2), together with two known compounds, aloenin aglycone (1) and aloin A (3), were fished out as the enzyme’s ligands. The structure of 2 was determined by HR-MS and comprehensive NMR techniques. Compound 3 exhibited a weak inhibitory effect on α-glucosidase, while compounds 1 and 2 were found to possess activation effects on the enzyme for the first time. It is interesting that both an inhibitor and agonists of α-glucosidase were fished out in one experiment.

Magnetic materials as adsorbents for the pre‐concentration and separation of active ingredients from herbal medicine

“…Yuan et al [74] immobilized α-glucosidase on functionalized Fe 3 O 4 for the selective enrichment of enzyme ligands present in Aloe vera and characterized the enzyme ligands by HPLC-MS. A new compound, aloinone, and two known compounds, aloin aglycone, and aloin A, were identified. The effect of these three compounds on α-glucosidase activity was investigated.…”

Magnetic Technologies and Green Solvents in Extraction and Separation of Bioactive Molecules Together with Biochemical Objects: Current Opportunities and Challenges

“…So magnetic solid-phase extraction (MSPE) has the advantage of simpler operation, is less time consuming, and has a more ideal performance, which can effectively avoid column packing and blocking; it is also easier to amplify than preparative chromatography. At present, MSPE has been widely used in chemical analysis, ligand fishing, biological separation, food science, genomics, proteomics and other fields [95][96][97].…”

Fast screening of α‐glucosidase inhibitors from Ginkgo biloba leaf by using α‐glucosidase immobilized on magnetic metal‐organic framework

“…α-Glucosidase promotes the transformation of carbohydrates into glucose for absorption by the body, thereby increasing postprandial blood glucose level [2,3]. Therefore, α-glucosidase is a key enzyme in the regulation of blood glucose and is widely considered as a target for antidiabetic drugs [4]. Diabetes is a chronic metabolic disorder characterized by inadequate production or response to insulin, resulting in elevated glucose levels in the bloodstream.…”