Preparation of Coralline Hydroxyapatite Implant with Recombinant Human Bone Morphogenetic Protein‐2‐Loaded Chitosan Nanospheres and Its Osteogenic Efficacy

Abstract

Spinal fusion is one of the most common surgical interventions for spine reconstruction. Despite the efforts to promote osteogenesis after spinal fusion, osteogenesis after spinal fusion remains a clinical challenge and new methods are still needed. The bone morphogenetic protein-2 (BMP-2) is a widely reported factor that can facilitate the osteogenesis in spinal fusion. In previous research, we found that the delivery of chitosan nanospheres could promote the effects of BMP-2 on osteogenic activity. The coralline hydroxyapatite (CHA) is one of the most frequently used implants in bone fusion. However, up to now no study has focused on the osteogenic efficacy of the CHA composite with recombinant human BMP-2 (rhBMP-2)-loaded chitosan nanospheres. This study aimed to investigate the effects of the CHA implant with rhBMP-2-loaded chitosan nanospheres on osteogenesis in spinal fusion. Methods: The rhBMP-2-loaded microspheres and CHA composite (rhBMP-2 microspheres/CHA) were prepared and were used for implantation of the rats. All SD rats were divided into four groups: the rhBMP-2 microspheres/CHA composite group (containing 0.5 mg rhBMP-2), the rhBMP-2-loaded CHA (rhBMP-2/CHA) composite group (containing 0.5 mg rhBMP-2), the blank CHA group, and the negative control group. The microsphere morphology was scanned and analyzed using a scanning electron microscope. Micro-computed tomography examination and three-dimensional reconstruction were performed 4 weeks after the surgery. Hematoxylin and eosin staining was conducted for histological analysis. Both alkaline phosphatase (ALP) and calcium content were measured. Results: The rhBMP-2-loaded CHA (rhBMP-2/CHA) composite was successfully prepared. Spherical regularity and a smooth and unwrinkled surface of the spheres were observed in all chitosan (CS)/rhBMP-2 microspheres. No side effects, infections, or abnormal behaviors were found in the animals. After 4 weeks of surgery, obvious new bone formation and bone fusion could be observed around the implant in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group. No ectopic osteogenesis was found in the vertebral canal or other muscle tissues. After 4 weeks of implantation, in both the rhBMP-2 microspheres/CHA composite group and the rhBMP-2/CHA composite group, osteoid tissues could be found, and bone cells, bone marrow, and trabecular bone turned into mature sclerotin, obvious bone tissue formation could be also seen. Both ALP activity and calcium content in the rhBMP-2 microspheres/CHA composite group (6.52 ± 0.50 kat/g and 17.54 ± 2.49 μg/mg) were significantly higher than in all other groups. Conclusion: The composite with rhBMP-2-loaded CS nanospheres could enhance osteogenic efficacy and increase the ALP activity and calcium content. These results might provide a novel method for osteogenesis in spinal fusion and offer new insight into the role of BMP-2 in osteogenesis.

pH-responsive cinnamaldehyde-TiO2 nanotube coating: fabrication and functions in a simulated diabetes condition

“…TNT can be used as favorable drug carrier because of its high surface-to-volume ratio [ 9 , 12 ]. Previously, loading of peptides [ 13 ], proteins [ 14 ], and nanoparticles [ 15 ] on TNT has been attempted; However, most of them are expensive, hard to preserve [ 16 ], and have a risk of adverse effects because of their narrow range of safe and effective concentration [ 17 ]. In the present, plant-derived extracts have gradually attracted the attention of researchers for their properties of better safety, ease of procurement, and fewer adverse effects [ 18 ].…”

Bone Regeneration by Multichannel Cylindrical Granular Bone Substitute for Regeneration of Bone in Cases of Tumor, Fracture, and Arthroplasty

“…A granular bone substitute could be used as an efficient filler, with ease of applicability and adequate conformity to the defect volume. However, optimum control of the interconnected porosity while maintaining strength is a persistent challenge for bone substitutes, especially for granular bone substitutes [ 23 ]. A cylindrical granule with multiple through channels could be a good choice, as the regular shape demonstrates strength and the through channels may facilitate easy cellular proliferation.…”

Combination of anthocyanin ternatin and hydroxyapatite/β-tricalcium phosphate coralline as a socket preservation biomaterial in dental implant: A narrative review

“…HA/β-TCP has high solubility properties which can potentially enhance osteogenesis, osteoconductivity, and osteoinduction. [8][9][10][11] The combination of anthocyanin ternatin and HA/β-TCP coralline acts as a scaffold which is applied to the defect area in the form of dried powder to induce regeneration bone, induce colonization of osteogenic cells, and control the inflammatory process in order to repair alveolar bone defect which provide good environment for dental implants insertion. [12] This narrative review is compiled to describe the mechanism of combination of anthocyanin ternatin and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) coralline as a socket preservation biomaterial in dental implants.…”