Preparation of Bovine Serum Albumin Surface-Imprinted Submicrometer Particles with Magnetic Susceptibility through Core−Shell Miniemulsion Polymerization

Abstract

Molecular imprinting is a state-of-the-art technique for preparing mimics of natural, biological receptors. Nevertheless, the imprinting of macromolecules like proteins remains a challenge due to their bulkiness and sensitivity to denaturation. In this work, a surface imprinting strategy based on covalently immobilized template molecules was adopted for protein imprinting. Bovine serum albumin (BSA) surface-imprinted submicrometer particles (500-600 nm) with magnetic susceptibility were prepared through a two-stage core-shell miniemulsion polymerization system using methyl methacrylate and ethylene glycol dimethacrylate as functional and cross-linking monomers, respectively. The particles possessed a novel red blood cell-like structure and exhibited a very favorable recognition property toward the template BSA molecules in aqueous medium. In a two-protein system, the particles had shown a very high specific recognition of the template proteins over the nontemplate proteins. The magnetic susceptibility was imparted through the successful encapsulation of Fe3O4 nanoparticles. Their superparamagnetic nature increases their potential applications in the fields such as magnetic bioseparation, cell labeling, and bioimaging. In addition, the importance of template immobilization for successful protein imprinting had also been illustrated to demonstrate the potential of this approach as a general methodology for protein imprinting.

Preparation of Alkanethiolate-Functionalized Core/Shell Fe3O4@Au Nanoparticles and Its Interaction with Several Typical Target Molecules

“…44 All of the XPS data agreed well with the results previously reported. 21,37,[42][43][44][45] On the basis of above results, it can be concluded that 11-MUA and 11-DDT monolayers were successfully formed on the surface of Fe 3 O 4 @ Au NPs.…”

Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin

“…And, the rebinding was primarily related to the specic recognition sites with high binding affinity for phycocyanin. 41 In contrast, the interaction of C-NIP with phycocyanin was mainly from nonspecic adsorption such as van der Waals. Moreover, it is well known that the Freundlich and Langmuir isotherm models are generally used to account for heterogeneous binding sites in MIPs.…”

Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins

“…This is an important consideration in the practical application of the magnetic materials in a separation process [24]. The adsorption kinetics of magnetic particles for BSA, Mb and Lyz under optimized pH showed a rapid increase, achieving 92% of the equilibrium adsorption capacity for BSA and ∼85% for Mb and Lyz, during the first 6 min.…”