Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins

Gai, Qingqing; Qu, Feng; Zhang, Tao; Zhang, Yukui

doi:10.1016/j.talanta.2011.03.055

Cited by 33 publications

(18 citation statements)

References 24 publications

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“…Among the most interesting characteristics of ATPE systems applied to purify proteins is the possibility of allowing a separation, in selective form, of one protein among a group, through partition between phases (Gai et al, 2011). In fact, Alcântara et al (2011) and Kalaivani and Regupathi (2015) demonstrated the feasibility of separation of αla from βlg with this type of strategy.…”

Section: Protein and Lactose Separationmentioning

confidence: 99%

“…In ATPE systems, a liquid-liquid separation occurs through mixtures of two polymers or a polymer and a salt, which in certain concentrations produce a true solution in a single phase, but in others they cause formation of two immiscible phases, among which the biomolecules present in the mixture are separated (Raja, Murty, Thivaharan, Rajasekar, & Ramesh, 2011). ATPE has been studied even to separate proteins in selective form (Gai, Qu, Zhang, & Zhang, 2011;Nitsawang, Hatti-Kaul, & Kanasawud, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Purification and concentration of cheese whey proteins through aqueous two phase extraction

Domínguez-Puerto

Valle‐Guadarrama

Guerra-Ramı́rez

et al. 2018

CyTA - Journal of Food

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Whey is a by-product of cheese manufacture and contains proteins that can be recovered, but lactose presence limits its use. Proteins can be separated with aqueous two-phase extraction (ATPE), thus the objective was to evaluate this strategy to recuperate proteins from whey attending lactose elimination. An ATPE system with (NH 4 ) 2 SO 4 and polyethylene glycol 4000 (PEG4000) was studied at 25°C. As PEG4000 diminished the volume ratio between phases diminished and a biphasic system was maintained in absence of the polymer. Minimum protein recovering and lactose elimination were 54.5% and 62.1% with mixtures having 25% PEG4000 and 12.1% (NH 4 ) 2 SO 4 , but values were 93.0% and 72.3%, respectively, with 0.1% PEG4000 and 34.7% (NH 4 ) 2 SO 4 . Therefore, whey incorporated with (NH 4 ) 2 SO 4 in concentration higher than 34% and without polyethylene glycol can be used in ATPE systems to maximize lactose elimination and protein recovering and to concentrate proteins up to 2.45 times through a non-energy strategy.Purificación y concentración de proteínas de suero de queso mediante extracción acuosa en dos fases RESUMEN El suero es un subproducto de la manufactura de queso y contiene proteínas que pueden ser recuperadas, pero la presencia de lactosa limita su uso. Las proteínas pueden ser separadas con extracción acuosa en dos fases (ATPE), por lo que el objetivo fue evaluar esta estrategia para recuperar proteínas del suero atendiendo la eliminación de lactosa. Se estudió un sistema ATPE formado con (NH 4 ) 2 SO 4 y polietilenglicol 4000 (PEG4000) a 25°C. A medida que PEG4000 disminuyó se redujo la relación de volúmenes entre fases y en ausencia del polímero se mantuvo un sistema bifásico. El rendimiento mínimo de recuperación de proteínas y eliminación de lactosa fueron 54.5% y 62.1% con mezclas de 25% PEG4000 y 12.1% (NH 4 ) 2 SO 4 , pero los valores fueron 93.0% y 72.3%, respectivamente, con 0.1% PEG4000 y 34.7% (NH 4 ) 2 SO 4 . Por lo tanto, el suero incorporado con (NH 4 ) 2 SO 4 en concentración mayor a 34% y sin polietilenglicol puede ser usado en un sistema ATPE para maximizar la eliminación de lactosa y la recuperación de proteínas y para concentrar proteínas hasta 2.45 veces a través de una estrategia no energética. ARTICLE HISTORY

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Section: Protein and Lactose Separationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Purification and concentration of cheese whey proteins through aqueous two phase extraction

Domínguez-Puerto

Valle‐Guadarrama

Guerra-Ramı́rez

et al. 2018

CyTA - Journal of Food

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“…Its isoelectric point is approx. 11.2 [21] and it is therefore supposed to bind to the cation exchanger groups of the particles (remaining carboxyl groups on the particle surface) over a broad pH range.…”

Section: Protein Binding Studiesmentioning

confidence: 99%

Controlling the partitioning behavior of magnetic micro-particles via hydrophobization with alkylamines: Tailored adsorbents for continuous bioseparation

Paulus

Till

Franzreb

2015

Applied Surface Science

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“…In this study, a hybrid method of AMTPS and MSPE named magnetic aqueous micellar two-phase system (MAMTPS) was developed for the extraction, enrichment and removal of dyes from aqueous solutions. Up to now, the similar methods were mainly reported for the purification of biomolecules, such as the protein [20], chymotrypsin [21], and antibody [18], but no relevant study hasbeen reported about their use in the extraction/isolation of small molecules contaminants from aqueous solution. Therefore, three common dyes (Congo red, methyl blue, and methyl violet) were chosen as the target compounds in this study, the influencing factors of type and amount of MNPs, surfactant concentration, vibration time, and extraction temperature were studied to obtain the optimal operation conditions.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Extraction, Enrichment and Removal of Dyes from Aqueous Solutions Using a Magnetic Aqueous Micellar Two-Phase System

Sun

Wang

et al. 2017

Applied Sciences

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Abstract:The magnetic aqueous micellar two-phase system (MAMTPS) has the advantages combined of magnetic solid phase extraction (MSPE) and aqueous micellar two-phase system (AMTPS). Thus, MAMTPS based on Fe 3 O 4 magnetic nanoparticles (MNPs) and a nonionic surfactant Triton X-114 (TX-114) was developed for the extraction, enrichment and removal of three dyes (Congo red, methyl blue, and methyl violet) from aqueous solutions in this study. The MNPs Fe 3 O 4 @NH 2 was screened as the optimal MNPs benefiting the extraction. Then, the influencing factors of MNPs amount, TX-114 concentration, vibration time, and extraction temperature were investigated in detail. The results showed that the extraction efficiencies of three dyes almost reached 100% using MAMTPS under the optimal conditions; MAMTPS had higher extraction ability than the individual MSPE or AMTPS. Thus, MAMTPS had the advantages of simple operation, high extraction ability, easy recycling of MNPs, and short phase-separation time, which showspotential for use in the extraction and analysis of contaminants from water samples.

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Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins

Cited by 33 publications

References 24 publications

Purification and concentration of cheese whey proteins through aqueous two phase extraction

Purification and concentration of cheese whey proteins through aqueous two phase extraction

Controlling the partitioning behavior of magnetic micro-particles via hydrophobization with alkylamines: Tailored adsorbents for continuous bioseparation

Simultaneous Extraction, Enrichment and Removal of Dyes from Aqueous Solutions Using a Magnetic Aqueous Micellar Two-Phase System

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