Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin

Zhang, Zhong; Li, Jinhua; Fu, Jiayi; Chen, Lingxin

doi:10.1039/c4ra00668b

Cited by 25 publications

(21 citation statements)

References 45 publications

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“…Recently, to overcome these problems, the focus has been on synthesizing monodispersed molecularly imprinted polymer particles (MIP‐NPs) with nanometer‐size range having a core–shell structure with molecular recognition cavities in the shell layer. This technique has attracted attention since the particles generally achieve high binding capabilities due to their large surface area and monodispersed particle size …”

Section: Introductionmentioning

confidence: 99%

Fluorescence Reporting of Binding Interactions of Target Molecules with Core–Shell‐Type Cortisol‐Imprinted Polymer Particles Using Environmentally Responsible Fluorescent‐Labeled Cortisol

Murase

Taniguchi

Takano

et al. 2015

Macro Chemistry & Physics

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Core-shell-type molecularly imprinted polymer particles (MIP-NPs) bearing specifi c binding cavities for cortisol in their shell layers by two-step emulsifi er-free emulsion polymerizations are synthesized, where both the strategies of semicovalent molecular imprinting and noncovalent molecular imprinting are concurrently performed using methacryloyl cortisol and itaconic acid as a template molecule and a functional monomer, respectively. Newly designed fl uorescent reporter molecules, dansyl-labeled cortisol, to investigate the binding capability of MIP-NPs toward cortisol by fl uorescence measurements are developed. The binding rate constant ( k a ) and affi nity constant ( K a ) of MIP-NPs toward cortisol are successfully estimated by curve fi tting for fl uorescence spectral shift. The k a value of MIP-NPs is 1.8 times larger than that of reference MIP-NPs prepared using only methacryloyl cortisol without itaconic acid (semicovalent imprinting), indicating that the concurrent demonstration of both the semicovalent imprinting and noncovalent imprinting processes is effective for constructing high affi nity binding cavities for cortisol.

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Section: Introductionmentioning

confidence: 99%

Fluorescence Reporting of Binding Interactions of Target Molecules with Core–Shell‐Type Cortisol‐Imprinted Polymer Particles Using Environmentally Responsible Fluorescent‐Labeled Cortisol

Murase

Taniguchi

Takano

et al. 2015

Macro Chemistry & Physics

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“…30 Besides the reported remote sensing methods, 30 in which empirical and semiempirical models are commonly used to estimate the relationship between phycocyanin concentrations and water quality, few measurement methods have been developed. 31,32 So, it is quite imperative to develop effective strategies for recognition and determination of phycocyanin.…”

Section: Introductionmentioning

confidence: 99%

Quantum Dots Based Mesoporous Structured Imprinting Microspheres for the Sensitive Fluorescent Detection of Phycocyanin

Zhong

Wang

et al. 2015

ACS Appl. Mater. Interfaces

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134

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Phycocyanin with important physiological/environmental significance has attracted increasing attention; versatile molecularly imprinted polymers (MIPs) have been applied to diverse species, but protein imprinting is still quite difficult. Herein, using phycocyanin as template via a sol−gel process, we developed a novel fluorescent probe for specific recognition and sensitive detection of phycocyanin by quantum dots (QDs) based mesoporous structured imprinting microspheres (SiO 2 @QDs@ms-MIPs), obeying electron-transfer-induced fluorescence quenching mechanism. When phycocyanin was present, a Meisenheimer complex would be produced between phycocyanin and primary amino groups of QDs surface, and then the photoluminescent energy of QDs would be transferred to the complex, leading to the fluorescence quenching of QDs. As a result, the fluorescent intensity of the SiO 2 @QDs@ms-MIPs was significantly decreased within 8 min, and accordingly a favorable linearity within 0.02−0.8 μM and a high detectability of 5.9 nM were presented. Excellent recognition specificity for phycocyanin over its analogues was displayed, with a high imprinting factor of 4.72. Furthermore, the validated probe strategy was successfully applied to seawater and lake water sample analysis, and high recoveries in the range of 94.0−105.0% were attained at three spiking levels of phycocyanin, with precisions below 5.3%. The study provided promising perspectives to develop fluorescent probes for convenient, rapid recognition and sensitive detection of trace proteins from complex matrices, and further pushed forward protein imprinting research.

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“…To circumvent the problem, magnetic particles (MPs) have drawn a great deal of interests in proteins separation [8][9][10][11] due to their unique magnetic response, high surface area, good dispersion and easy surface modication. The MPs-based magnetic separation can be directly performed on complex samples.…”

Section: Introductionmentioning

confidence: 99%

Preparation of magnetic metal organic framework nanocomposites for efficient and selective adsorption of hemoglobin from bovine blood

Yao

Gao

et al. 2017

RSC Adv.

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Fluorescent and magnetic dual-responsive coreshell imprinting microspheres strategy for recognition and detection of phycocyanin

Cited by 25 publications

References 45 publications

Fluorescence Reporting of Binding Interactions of Target Molecules with Core–Shell‐Type Cortisol‐Imprinted Polymer Particles Using Environmentally Responsible Fluorescent‐Labeled Cortisol

Fluorescence Reporting of Binding Interactions of Target Molecules with Core–Shell‐Type Cortisol‐Imprinted Polymer Particles Using Environmentally Responsible Fluorescent‐Labeled Cortisol

Quantum Dots Based Mesoporous Structured Imprinting Microspheres for the Sensitive Fluorescent Detection of Phycocyanin

Preparation of magnetic metal organic framework nanocomposites for efficient and selective adsorption of hemoglobin from bovine blood

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