Preparation of Antioxidant Peptide by Microwave- Assisted Hydrolysis of Collagen and Its Protective Effect Against H2O2-Induced Damage of RAW264.7 Cells

Abstract

Antioxidant peptides have elicited interest for the versatility of their use in the food and pharmaceutical industry. In the current study, antioxidant peptides were prepared by microwave-assisted alkaline protease hydrolysis of collagen from sea cucumber (Acaudina molpadioides). The results showed that microwave irradiation significantly improved the degree of hydrolysis of collagen and the hydroxyl radical (OH⋅) scavenging activity of hydrolysate. The content and OH⋅ scavenging activity of collagen peptides with molecular weight ≤ 1 kDa (CPS) in the hydrolysate obtained at 250 W increased significantly compared with the non-microwave-assisted control. CPS could scavenge OH⋅ and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical in a dose-dependent manner. The scavenging activity of OH⋅ and DPPH radical was 93.1% and 41.2%, respectively, at CPS concentration of 1 mg/mL. CPS could significantly promote RAW264.7 cell proliferation and reduce the Reactive Oxygen Species (ROS) level of H2O2-induced damage in RAW264.7 cells in a dose-dependent manner. Furthermore, all CPS-treated groups exhibited an increase in superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and a decrease in malondialdehyde (MDA) level compared with the control. These results showed that CPS could effectively protect RAW264.7 cells from H2O2-induced damage, implying the potential utilization of CPS as a natural antioxidant for food and pharmaceutical applications.

Collagen Peptides from Swim Bladders of Giant Croaker (Nibea japonica) and Their Protective Effects against H2O2-Induced Oxidative Damage toward Human Umbilical Vein Endothelial Cells

“…These results indicate that the protective effect of SNNH-1 on H 2 O 2 -induced HUVECs injury may be due to the inhibition of intracellular ROS production. These results are in accordance with the data presented by Li et al [ 22 ], who found that collagen peptides from sea cucumbers ( Acaudina molpadioides ) could effectively protect cells from H 2 O 2 -induced damage.…”

“…As shown in Figure 5 , peptide fractions of less than 1 kDa had the highest DPPH free radical scavenging rate compared to the other ultrafiltration fractions. Similar research has shown that the low-Mw content of protein hydrolysates has higher antioxidant activity [ 22 ]. Therefore, fractions with a Mw of less than 1 kDa were chosen for subsequent activity evaluation and named SNNH-1.…”

Cytoprotective Effects of Fish Protein Hydrolysates against H2O2-Induced Oxidative Stress and Mycotoxins in Caco-2/TC7 Cells

“…However, SNNH-1 pretreatment reduced ROS levels in a concentration-dependent manner on HUVECs cells. These results were in agreement with the data presented by Li et al [ 65 ], who found that collagen peptides from sea cucumbers ( Acaudina molpadioides ) could protect RAW264.7 cells from H 2 O 2 -induced damage. Hu et al [ 58 ] observed the effects of MMP-4, MMP-7, and MMP-12 (three peptides of hydrolysate of monkfish, Lophius litulon, muscle), on the level of ROS in oxidative damage HepG2 cells.…”

Optimization of Extraction of Bioactive Peptides from Monkfish (Lophius litulon) and Characterization of Their Role in H2O2-Induced Lesion

“…After culturing for 24 h, the medium was gently aspirated and replaced with LPs <1 kDa at different concentrations (12.5, 25, 50, 100, 200, 400, 600, 800, and 1000 μg/mL). Cells were cultured in the medium for 24 h and treated with H 2 O 2 (final concentration of 500 μmol/L) for 8 h [ 38 ]. Then, 20 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was added into the culture solution.…”