Cytoprotective Effects of Fish Protein Hydrolysates against H2O2-Induced Oxidative Stress and Mycotoxins in Caco-2/TC7 Cells

Abstract

Many studies report the potent antioxidant capacity for fish protein hydrolysates, including radical scavenging activity and inhibition ability on lipid peroxidation (LPO). In this study, the in vitro cytotoxicity of protein hydrolysates from different salmon, mackerel, and herring side streams fractions was evaluated in the concentration range from 1 to 1:32 dilution, using cloned human colon adenocarcinoma cells TC7 (Caco-2/TC7) by MTT and PT assays. The protein hydrolysates’ antioxidant capacity and oxidative stress effects were evaluated by LPO and reactive oxygen species (ROS) generation, respectively. The antioxidant capacity for pure and bioavailable hydrolysate fraction was also evaluated and compared. Additionally, mycotoxin levels were determined in the fish protein hydrolysates, and their cytoprotective effect against T-2 toxin was evaluated. Both hydrolysates and their bioavailable fraction induced similar cell viability rates. The highest cytoprotective effect was obtained for the salmon viscera protein hydrolysate (HSV), which increased the cell viability by 51.2%. ROS accumulation induced by H2O2 and LPO was suppressed by all pure hydrolysates. The cytoprotective effect of hydrolysates was observed against T-2. Moreover, the different fish fraction protein hydrolysates contain variable nutrients and unique bioactive peptide composition showing variable bioactivity, which could be a useful tool in developing dietary supplements with different target functional properties.

Comparative Cytotoxic Effects and Possible Mechanisms of Deoxynivalenol, Zearalenone and T-2 Toxin Exposure to Porcine Leydig Cells In Vitro

“…In this study, we found that T-2 was the most cytotoxic in Leydig cells (IC 50 : 97.18 nM), followed by DON (IC 50 : 2.49 µM) and ZEN (IC 50 : 49.71 µM). Among mycotoxins produced by Fusarium species, T-2 is considered to be the most potent in terms of cytotoxicity [20]. This study is the first to assess the impact of different concentrations of ZEN, DON and T-2 on the viability of porcine Leydig cells.…”

Enhancement of the Antioxidant Effect of Natural Products on the Proliferation of Caco-2 Cells Produced by Fish Protein Hydrolysates and Collagen

“…The effective use of these fish side streams contributes to the generation of high-added-value food products for industry. The extracts and fish protein hydrolyzates can have different applications, including use as antimicrobials, antioxidants and dyes to improve the nutritional profile of foods [ 21 ]. Most authors have determined that fish protein hydrolyzates and fish collagen are great candidates for the development of dietary supplements because of their numerous benefits.…”

“…The beneficial effects of hydrolysates have been outlined by Taroncher et al [ 21 ]. Fish and their derived products are considered as a good nutritional source because of their high mineral content.…”

Bioactive Antimicrobial Peptides from Food Proteins: Perspectives and Challenges for Controlling Foodborne Pathogens

“…In addition, recent studies have shown the protective effects of BAPs against the cytotoxicity of the Fusarium mycotoxins deoxynivalenol (DON) and T-2 toxin, mainly associated with the decrease of oxidative stress [ 165 , 166 ]. This is also interesting for both food and feed applications since Fusarium is a ubiquitous fungal genus that infects crops and produces several types of mycotoxins—which are often found contaminating cereals and cereal-based products [ 167 , 168 ].…”