Optimization of Extraction of Bioactive Peptides from Monkfish (Lophius litulon) and Characterization of Their Role in H2O2-Induced Lesion

Abstract

Background: Marine fish meat has been widely used for the extraction of bioactive peptides. This study was aimed to optimize the preparation of monkfish muscle peptides (LPs) using response surface methodology (RSM) and explore the antioxidant activities of <1 kDa LPs. Methods: Peptides were prepared from the muscles of monkfish (Lophius litulon), and five proteases were tested to hydrolyze muscle proteins. The hydrolysate that was treated using neutrase showed the highest degree of hydrolysis (DH) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities. Results: The optimized conditions were as follows: water/material ratio of 5.4:1, a time span of 5 h, pH of 7.0, enzyme concentration of 2000 U/g, and temperature of 45 °C; the maximum DPPH scavenging activity and DH were 92.861% and 19.302%, respectively. LPs exhibited appreciable antioxidant activities, including DPPH radical, hydroxyl radical, 2,2′-azinobis-3-ethylbenzthiazoline-6-sulphonate (ABTS) radical, and superoxide anion scavenging activities. LPs attenuated H2O2-related oxidative injury in RAW264.7 cells, reduced the reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and increased the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and catalase (CAT) levels. Conclusion: We concluded that LPs could be an ideal source of bioactive peptides from monkfish and also have pharmaceutical potential.

Antioxidant Activity of Hybrid Sturgeon (<i>Huso dauricus</i> × <i>Acipenser schrenckii</i>) Protein Hydrolysate Prepared Using Bromelain, Its Fractions and Purified Peptides

“…The results showed that the antiradical activity of BH against ABTS •+ ranged from 23.09% at 1 mg/mL to 62.07% at 5 mg/mL with IC 50 of 3.81 mg/mL. Concentrationdependent activity in the ABTS assay was consistent with previous results, which found that increased fish protein hydrolysate concentrations resulted in increased antioxidant activity [Chalamaiah et al, 2013;Tian et al, 2020]. Latorres et al [2018] reported that the ability of protein hydrolysate to scavenge the ABTS •+ was associated with the size of the peptide chain and the enzyme specificity to break the peptide bonds during the enzymolysis.…”

Using Collagen Peptides From the Skin of Monkfish (Lophius litulon) to Ameliorate Kidney Damage in High-Fat Diet Fed Mice by Regulating the Nrf2 Pathway and NLRP3 Signaling

“…We selected acid protease to hydrolyze monkfish skin based on its efficiency. Unlike in our previously published research, we found neutrase to be the best enzyme for preparing peptides derived from monkfish meat (31). This may be attributed to the disparities in the protein composition.…”

Monkfish Peptides Mitigate High Fat Diet-Induced Hepatic Steatosis in Mice

“…We previously found that LPs had a high antioxidant activity and could effectively scavenge DPPH, hydroxyl, ABTS, and superoxide anion radicals, and protect RAW264.7 cells from H 2 O 2 -induced injury. LPs can be used as raw materials for natural antioxidants [ 26 ]. In order to provide a theoretical basis for the stable development and application of LPs and related products, we systematically analyzed the composition of LPs and identified the corresponding proteins using both database search and de novo sequencing methods.…”

“…Monkfish meat has traditionally been considered a low-value food. Monkfish peptides (LPs) have been found to have strong antioxidant activities and fat absorption capabilities [ 26 , 27 , 28 ]. However, the regulatory effects of LPs on NAFLD, and the underlying molecular mechanisms are still unclear.…”