Preparation, characterization, antioxidant and anti-inflammatory activities of acid-soluble pectin from okra (Abelmoschus esculentus L.)

Xiong, Baoyi; Zhang, Wencheng; Wu, Zeyu; Li, Rui; Yang, Chengying; Hui, Ailing; Huang, Xusheng; Xian, Zhaojun

doi:10.1016/j.ijbiomac.2021.03.202

Cited by 50 publications

(28 citation statements)

References 78 publications

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“…Despite previous studies demonstrating that A. esculentus has anti-inflammatory, anti-diabetic, anti-tumor, anti-bacterial, and anti-oxidant activities, its immune-stimulatory activity has not thus far been studied. 12 , 13 , 14 , 15 This is the ﬁrst study to describe the immune-stimulatory activity of A. esculentus water extract in RAW264.7 macrophages. Macrophages are important components of the host defense system, and are involved at all stages of the immune response.…”

Section: Discussionmentioning

confidence: 99%

“…Owing to these effects, okra has also been referred to as “green ginseng”. 12 , 13 , 14 , 15 According to reports on its nutritional composition and phytochemical structure, A. esculentus contains a number of antioxidant vitamins (e.g., folate, niacin, choline, A, K, E, and β-carotene) as well as lipids, minerals, carbohydrates, dietary fibers, and amino acids. Moreover, it also contains health-promoting phenolics and flavonoids such as epigallocatechin, myricetin, gossypetin-8-O-β-D-glucuronide, quercetin glycosides and other quercetin derivatives and its glycosides, and gossypetin-8-O-β-D-glucuronide.…”

Section: Introductionmentioning

confidence: 99%

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Immunomodulatory effects of Abelmoschus esculentus water extract through MAPK and NF-κB signaling in RAW 264.7 cells

Hyun

Ahn

et al. 2022

Biotechnology Notes

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immunomodulatory effects of Abelmoschus esculentus water extract through MAPK and NF-κB signaling in RAW 264.7 cells

Hyun

Ahn

et al. 2022

Biotechnology Notes

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“…It was reported that the acid-soluble pectin isolated from edible okra ( Abelmoschus esculentus ) had both anti-inflammatory and anti-oxidant potentials, and thereby showed a capacity to reduce NO formation and inflammatory cytokines secretion in the LPS-treated RAW 264.7 macrophages [ 36 ]. Meanwhile, the pectin from okra stem had the anti-fatigue activity by increasing both blood glucose and glycogen levels in the body [ 37 ]. In addition, the okra polysaccharides had anti-inflammation via inhibiting the phosphorylation of IκB and p65 proteins and reducing the secretion of inflammatory cytokines in the LPS-treated RAW 264.7 macrophages [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

Lin

Guan

et al. 2021

Foods

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The soluble polysaccharides from a non-conventional and edible plant purslane (Portulaca oleracea L.), namely PSPO, were prepared by the water extraction and ethanol precipitation methods in this study. The obtained PSPO were selenylated using the Na2SeO3-HNO3 method to successfully prepare two selenylated products, namely SePSPO-1 and SePSPO-2, with different selenylation extents. The assay results confirmed that SePSPO-1 and SePSPO-2 had respective Se contents of 753.8 and 1325.1 mg/kg, while PSPO only contained Se element about 80.6 mg/kg. The results demonstrated that SePSPO-1 and SePSPO-2 had higher immune modulation than PSPO (p < 0.05), when using the two immune cells (murine splenocytes and RAW 264.7 macrophages) as two cell models. Specifically, SePSPO-1 and SePSPO-2 were more active than PSPO in the macrophages, resulting in higher cell proliferation, greater macrophage phagocytosis, and higher secretion of the immune-related three cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β. Meanwhile, SePSPO-1 and SePSPO-2 were more potent than PSPO in the concanavalin A- or lipopolysaccharide-stimulated splenocytes in cell proliferation, or more able than PSPO in the splenocytes to promote interferon-γ secretion but suppress IL-4 secretion, or more capable of enhancing the ratio of T-helper (CD4+) cells to T-cytotoxic (CD8+) cells for the T lymphocytes than PSPO. Overall, the higher selenylation extent of the selenylated PSPO mostly caused higher immune modulation in the model cells, while a higher polysaccharide dose consistently led to the greater regulation effect. Thus, it is concluded that the employed chemical selenylation could be used in the chemical modification of purslane or other plant polysaccharides, when aiming to endow the polysaccharides with higher immuno-modulatory effect on the two immune cells.

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“…Natural polysaccharides such as glucan [ 74 ], inulin [ 75 ] and pectins [ 78 ] have been shown to have strong anti-inflammatory activity. As demonstrated by Bermudez-Brito et al [ 63 ], pectic polysaccharides exhibit higher anti-inflammatory activity than inulin and glucan.…”

Section: Polysaccharides From Agricultural Wastementioning

confidence: 99%

Strategies to Increase the Biological and Biotechnological Value of Polysaccharides from Agricultural Waste for Application in Healthy Nutrition

Rivas

Casquete

Martı́n

et al. 2021

IJERPH

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Nowadays, there is a growing interest in the extraction and identification of new high added-value compounds from the agro-food industry that will valorize the great amount of by-products generated. Many of these bioactive compounds have shown beneficial effects for humans in terms of disease prevention, but they are also of great interest in the food industry due to their effect of extending the shelf life of foods by their well-known antioxidant and antimicrobial activity. For this reason, an additional research objective is to establish the best conditions for obtaining these compounds from complex by-product structures without altering their activity or even increasing it. This review highlights recent work on the identification and characterization of bioactive compounds from vegetable by-products, their functional activity, new methodologies for the extraction of bioactive compounds from vegetables, possibly increasing their biological activity, and the future of the global functional food and nutraceuticals market.

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Preparation, characterization, antioxidant and anti-inflammatory activities of acid-soluble pectin from okra (Abelmoschus esculentus L.)

Cited by 50 publications

References 78 publications

Immunomodulatory effects of Abelmoschus esculentus water extract through MAPK and NF-κB signaling in RAW 264.7 cells

Immunomodulatory effects of Abelmoschus esculentus water extract through MAPK and NF-κB signaling in RAW 264.7 cells

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

Strategies to Increase the Biological and Biotechnological Value of Polysaccharides from Agricultural Waste for Application in Healthy Nutrition

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