Preparation, characterization, and in vitro release kinetics of doxorubicin-loaded magnetosomes

Zhang, Shuang; Fan, Xinxin; Zhang, Guojing; Wang, Weidong; Yan, Lei

doi:10.1177/08853282211060544

Cited by 10 publications

(8 citation statements)

References 59 publications

(113 reference statements)

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“…31 It has been reported that magnetosomes can be synthesized by MTB such as Magnetospirillum magnetotacticum , Magnetospirillum gryphiswaldense and Magnetospirillum magneticum , and non-MTB such as A. ferrooxidans , Ferroplasma thermophilum and Leptospirillum ferrooxidans . 21 In this work, non-MTB A. ferrooxidans BYM was employed to produce magnetosomes in airlift bioreactor. Results showed the magnetosome yield of A. ferrooxidans BYM is of the same order of magnitude or lower than that reported for MTB (Table 4).…”

Section: Discussionmentioning

confidence: 99%

“…29 In the contrary, A. ferrooxidans BYM can grow in the low-cost medium and non-fastidious culture conditions, which is favored for industrial production of magnetosomes. 21,32…”

Section: Discussionmentioning

confidence: 99%

“…29 In the contrary, A. ferrooxidans BYM can grow in the low-cost medium and non-fastidious culture conditions, which is favored for industrial production of magnetosomes. 21,32 The particle size of magnetosomes can be assayed by TEM and SEM. The TEM image showed the spherical magnetosomes dispersed in A. ferrooxidans BYM (Figure 2), which was in agreement with the results in the previous studies showing that magnetosome arrangements were regularly scattered in A. ferrooxidans strains ATCC23270 and BY-3.…”

Section: Discussionmentioning

confidence: 99%

“…The nylon membrane was rinsed with 1 m H 2 SO 4 (pH 2.0) twice and then the suspension was centrifuged 3 times at 2000 r/min for 5 min at 4 o C. Finally, the precipitates were suspended in 0 K liquid medium (0.24 g/L (NH 4 ) 2 SO 4 , 0.1 g/L KCl, 0.5 g/L K 2 HPO 4 , 0.5 g/L MgSO 4 ·7H 2 O, 0.01 g/L Ca(NO 3 ) 2 and 12 mmol/L C 6 H 12 O 7 , the pH was adjusted to 2.0 with 1 m H 2 SO 4 at room temperature) and stored at −20 o C until further use. 21…”

Section: Methodsmentioning

confidence: 99%

“…The nylon membrane was rinsed with 1 M H 2 SO 4 (pH 2.0) twice and then the suspension was centrifuged 3 times at 2000 r/min for 5 min at 4 o C. Finally, the precipitates were suspended in 0 K liquid medium (0.24 g/L (NH 4 ) 2 SO 4 , 0.1 g/L KCl, 0.5 g/L K 2 HPO 4 , 0.5 g/L MgSO 4 Á7H 2 O, 0.01 g/L Ca(NO 3 ) 2 and 12 mmol/L C 6 H 12 O 7 , the pH was adjusted to 2.0 with 1 M H 2 SO 4 at room temperature) and stored at À20 o C until further use. 21 The magnetosomes were isolated according to our previous method. 22 In brief, a 5 g of A. ferrooxidans BYM were suspended in 20 mL of Tris-HCl buffer (10 mM, pH 7.4) containing 0.1 mM phenylmethylsulfonyl fluoride (PMSF) and incubated in a 37 o C water bath for 45 min.…”

Section: Extraction and Characterization Of Magnetosomesmentioning

confidence: 99%

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Magnetosome yield characteristics modeling of acidithiobacillus ferrooxidans in airlift bioreactor using response surface methodology

Yang

Fan

et al. 2022

J Biomater Appl

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Bacterial magnetosomes had been proved to have great application potential in medicine and biotechnology. The objective of the present study was to obtain high yield of magnetosomes from Acidithiobacillus ferrooxidans ( A. ferrooxidans) BYM in an airlift bioreactor using response surface methodology (RSM). The magnetosomes from A. ferrooxidans BYM were characterized using a transmission electron microscope and scanning electron microscopy. The maximum magnetosome yield of 0.4267 mg/L was achieved at ventilation capacity of 3.6 L/min and gluconic acid concentration of 10 mmol/L at 25oC. The correlation coefficient (R2) value of 0.8676 of the obtained model suggested a good correlation between the actual and predicted magnetosome yield. The confirmation experiment confirmed that the actual magnetosome yield of 0.391 mg/L obtained were in agreement with the predicted value of 0.398 mg/L. These results suggested that RSM can be employed to find out the optimum conditions for magnetosome formation in airlift bioreactor.

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Section: Discussionmentioning

confidence: 99%

“…29 In the contrary, A. ferrooxidans BYM can grow in the low-cost medium and non-fastidious culture conditions, which is favored for industrial production of magnetosomes. 21,32…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Extraction and Characterization Of Magnetosomesmentioning

confidence: 99%

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Magnetosome yield characteristics modeling of acidithiobacillus ferrooxidans in airlift bioreactor using response surface methodology

Yang

Fan

et al. 2022

J Biomater Appl

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A Printable Hydrogel Loaded with Medicinal Plant Extract for Promoting Wound Healing

Wang,

Zhang,

Song

et al. 2024

Adv Healthcare Materials

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How to promote wound healing is still a major challenge in the healthcare while macrophages are a critical component of the healing process. Compared to various bioactive drugs, many plants have been reported to facilitate the wound healing process by regulating the immune response of wounds. In this work, a 3D printed hydrogel scaffold loaded with natural Centella asiatica extract (CA extract) is developed for wound healing. This CA@3D scaffold uses gelatin and sodium alginate with CA extract as bio‐ink for 3D printing. The CA extract contains a variety of bioactive compounds that make the various active ingredients in Centella asiatica work in concert. The printed CA@3D scaffold can fit the shape of wound, orchestrate the macrophages and immune responses within the wound, and promote wound healing compared to commercial wound dressings. The underlying mechanism of promoting wound healing was also illuminated by applying multi‐omic analyses. Moreover, the CA extract loaded 3D scaffold also showed great ability to promote wound healing in diabetic chronic wounds. Due to its ease of preparation, low‐cost, biosafety, and therapeutic outcomes, this work proposes an effective strategy for promoting chronic wound healing.This article is protected by copyright. All rights reserved

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Redox-responsive degradable microgel modified with superparamagnetic nanoparticles exhibiting controlled, hyperthermia-enhanced drug release

et al. 2023

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A novel degradable microgel based on poly(N-isopropylacrylamide) (pNIPA) cross-linked with N,N’-bisacryloylcystine (BISS) and containing superparamagnetic iron oxide nanoparticles (SPION@CA) was synthesized by semi-batch precipitation polymerization and examined as a potential hyperthermia-enhanced drug carrier. The pNIPA provided the microgel with temperature sensitivity, the BISS was responsible for degradation in the presence of glutathione (GSH) (an –S–S–bond reductor naturally present in cells), while the SPION@CA permitted remote control of temperature to improve drug release. The microgels exhibited volume phase transition temperature at ca. 34 °C, which is near the human body temperature, and were stable across a wide range of temperatures and ionic strengths, as well as in the blood plasma at 37 °C. It was found that the presence of SPION@CA in the polymer network of the microgels enabled the temperature to be increased up to 42 °C by an alternating magnetic field, and that increasing the temperature from 37 to 42 °C significantly enhanced the releasing of the anticancer drug doxorubicin (DOX). The highest DOX release (82%) was observed at pH 5, 42 °C, and in the presence of GSH, and the lowest (20%) at pH 7.4, 37 °C, and in the absence of GSH. MTT assay indicated that compared to free doxorubicin, the microgel particles loaded with doxorubicin have comparable cytotoxicity against MCF-7 cancer cells while being significantly less toxic to MCF-10A healthy cells. Graphical abstract

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Preparation, characterization, and in vitro release kinetics of doxorubicin-loaded magnetosomes

Cited by 10 publications

References 59 publications

Magnetosome yield characteristics modeling of acidithiobacillus ferrooxidans in airlift bioreactor using response surface methodology

Magnetosome yield characteristics modeling of acidithiobacillus ferrooxidans in airlift bioreactor using response surface methodology

A Printable Hydrogel Loaded with Medicinal Plant Extract for Promoting Wound Healing

Redox-responsive degradable microgel modified with superparamagnetic nanoparticles exhibiting controlled, hyperthermia-enhanced drug release

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