Magnetosome yield characteristics modeling of acidithiobacillus ferrooxidans in airlift bioreactor using response surface methodology

Abstract

Bacterial magnetosomes had been proved to have great application potential in medicine and biotechnology. The objective of the present study was to obtain high yield of magnetosomes from Acidithiobacillus ferrooxidans ( A. ferrooxidans) BYM in an airlift bioreactor using response surface methodology (RSM). The magnetosomes from A. ferrooxidans BYM were characterized using a transmission electron microscope and scanning electron microscopy. The maximum magnetosome yield of 0.4267 mg/L was achieved at ventilation capacity of 3.6 L/min and gluconic acid concentration of 10 mmol/L at 25oC. The correlation coefficient (R2) value of 0.8676 of the obtained model suggested a good correlation between the actual and predicted magnetosome yield. The confirmation experiment confirmed that the actual magnetosome yield of 0.391 mg/L obtained were in agreement with the predicted value of 0.398 mg/L. These results suggested that RSM can be employed to find out the optimum conditions for magnetosome formation in airlift bioreactor.

Application of a Magnetic Field to Enhance the Environmental Sustainability and Efficiency of Microbial and Plant Biotechnological Processes

“…In the past, it has been demonstrated that even some non-magnetotactic bacteria, including Magnetospirillum aberrantis, A. ferrooxidans, and Leptospirillum ferriphilum, can perceive a magnetic field and produce magnetosomes [65,99]. It is also known that A. ferrooxidans does not exhibit magnetotaxis in the presence of the Earth's magnetic field, while weak magnetotaxis can be observed in an artificially induced magnetic field [65].…”

Phenomic and transcriptomic analyses reveal the sequential synthesis of Fe 3 O 4 nanoparticles in Acidithiobacillus ferrooxidans BYM

“…The mass cultivation of A. ferrooxidans has been easily available with enough Fe(II) ( 9 ). Thus, A. ferrooxidans is conferring greater flexibility and potential application in biomagnetic nanoparticle production ( 11 ).…”

“…However, current researches on Fe 3 O 4 nanoparticle synthesis in Acidithiobacillia spp. were only at the cellular level, but the topic on its molecular mechanism remains understudied ( 11 ). It is necessary to find potential key genes by multi-omic analysis and verify these genes by establishing genetic operation system, such as gene overexpression and knockout.…”