Preparation, characterization and in vitro–in vivo evaluation of bortezomib supermolecular aggregation nanovehicles

Abstract

Backgrounds: Intolerable toxicity and unsatisfactory therapeutic effects are still big problems retarding the use of chemotherapy against cancer. Nano-drug delivery system promised a lot in increasing the patients' compliance and therapeutic efficacy. As a unique nano-carrier, supermolecular aggregation nanovehicle has attracted increasing interests due to the following advantages: announcing drug loading efficacy, pronouncing in vivo performance and simplified production process. Methods: In this study, the supermolecular aggregation nanovehicle of bortezomib (BTZ) was prepared to treat breast cancer. Results: Although many supermolecular nanovehicles are inclined to disintegrate due to the weak intermolecular interactions among the components, the BTZ supermolecules are satisfying stable. To shed light on the reasons behind this, the forces driving the formation of the nanovehicles were detailed investigated. In other words, the interactions among BTZ and other two components were studied to characterize the nanovehicles and ensure its stability. Conclusions: Due to the promising tumor targeting ability of the BTZ nanovehicles, the supermolecule displayed promising tumor curing effects and negligible systemic toxicity.

Ciclopirox and bortezomib synergistically inhibits glioblastoma multiforme growth via simultaneously enhancing JNK/p38 MAPK and NF-κB signaling

“…BTZ has a cytotoxic effect in a variety of tumor cells 21 and can reduce the therapeutic concentration of tumor necrosis factor-related apoptosis-inducing ligand, for treating GBM 22 . To determine whether BTZ reduces the concentration of CPX required for its effects in GBM cells, we combined BTZ with CPX to treat U251 and SF126 cells.…”

Curcumin-incorporated 3D bioprinting gelatin methacryloyl hydrogel reduces reactive oxygen species-induced adipose-derived stem cell apoptosis and improves implanting survival in diabetic wounds

“…ADSCs were incubated in the solution (5 μL of annexin V-mCherry and 1 μL of GreenNuc™ caspase-3 substrate per 200 μL of binding buffer) for 30-45 min at 37°C without light. Cell membrane permeability Caspase-3 Substrate GreenNuc™ for the detection of caspase-3 activity in cultured cells can be used for real-time monitoring [ 26 ]. Immunofluorescence microscopy was used to obtain images (GreenNuc™, excitation/emission: 500/530 nm; Annexin V-mCherry, excitation/emission: 587/610 nm).…”

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

“…However, in vivo experiments showed that mice treated with BTZ alone were characterized by weight loss while the pVP coated BTZ-NPs led to weight gain in mice. This phenomenon indicated the reduced systemic toxicity of the BTZ-NPs [ 83 ].…”

“…( c ) Antidegradation action of pVP. Adapted with permission from [ 83 ], Springer, 2020. ( d ) Scheme of the action of nanoparticles obtained by two different methods, and graph showing enhancement of cisplatin toxicity through increased intercellular accumulation of drug bonded with NP compared to free cisplatin (*—statistically significant difference ( p < 0.05) from free cisplatin, **—statistically significant difference from M2-NPs).…”