Preparation and Hepatoprotective Activities of Peptides Derived from Mussels (Mytilus edulis) and Clams (Ruditapes philippinarum)

Wang, Qian; Liu, Fujun; Wang, Xin-Miao; Zhao, Guanhua; Dong, Chen; Yu, Jing-Han; Yin, Fawen; Zhou, Da‐Yong

doi:10.3390/md20110719

Cited by 5 publications

(2 citation statements)

References 69 publications

(104 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Likewise, two peptides NEDNHPGALGEPV and KVLPVPQQMVPYPRQ from camel milk protein, shown antioxidant activity against DPPH • (IC 50 0.04 and 0.02 mg/mL), • OH (IC 50 0.05 and 0.05 mg/mL), ABTS •+ (IC 50 0.1 and 0.01 mg/mL), and O 2 •- (IC 50 0.045 and 0.3 mg/mL) [51] . In addition, previous research have found that the antioxidant peptides were exploited their bioactive peptides to reduce the production of reactive oxygen species through activating intestinal absorption and metabolism by endogenous cellular and in vivo animal models [52] , [53] .…”

Section: Resultsmentioning

confidence: 99%

Molecular and biotechnological characteristics of proteolytic activity from Streptococcus thermophilus as a proteolytic lactic acid bacteria to enhance protein-derived bioactive peptides

et al. 2023

View full text Add to dashboard Cite

<abstract> The demand for healthy food items with a high nutrient value of bioavailability and bioaccessibility has created a need for continuous development of technology and food ingredients like bioactive peptides. This study aimed to investigate seven proteolytic lactic acid bacteria (PLABs) isolated from the <italic>plaa-som</italic> (fermented fish) sample originated from silver BARB species for production of proteolytic enzymes. Proteolytic enzymes produced by (PLABs) were used further to create potent bioactive peptides by hydrolyzing proteins throughout PLAB-probiotics enhancer. Protein derived-bioactive peptides was tested the proteolytic activity on different protein sources and examined bioactivities including antioxidative and antimicrobial effect for further use in functional foods. Results of screened-PLAB strains showed high proteolytic activity namely <italic>Streptococcus thermophilus</italic> strains (KKUPA22 and KKUPK13). These strains have proteolytic system consisting of extracellular and cell-bound enzymes that used for degrading protein in fish flesh protein (FFP) and skim milk (SKM) broth media. Proteolytic activity of tested bacterial enzymes was estimated after incubation at 45, 37, and 50 °C. Furthermore, FFP hydrolysates were formed with various peptides and has small molecular weights (checked by SDS-PAGE) in the range of10.5 to 22 kDa), exhibiting strong activity. Data revealed that <italic>S. thermophilus</italic> strains (KKUPA22 and KKUPK13) had high antioxidant activity in term of 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical-scavenging inhibition, and ferric reducing antioxidant power (FRAP) reducing power capacity. Both strains (KKUPA22 and KKUPK13) of <italic>S. thermophilus</italic> have higher antimicrobial activity against Gram-negative bacteria than against Gram-positive bacteria. We have confirmed presence of proteolytic (<italic>prt</italic>) gene regions in <italic>S. thermophilus</italic> strains using specific primers via PCR amplification. Results showed highest homology (100%) with the <italic>prt</italic>S gene of <italic>S. thermophillus</italic> located on the cell envelope proteolytic enzymes (CEPEs) such as serine proteinase. Therefore, it concluded that the proteolytic system of tested PLAB strains able to generate bioactive peptides-derived proteins having active biological property, good mechanism of degradability, and bioaccessibility for further use in catalyzing protein of functional foods. </abstract>

show abstract

Section: Resultsmentioning

confidence: 99%

Molecular and biotechnological characteristics of proteolytic activity from Streptococcus thermophilus as a proteolytic lactic acid bacteria to enhance protein-derived bioactive peptides

et al. 2023

View full text Add to dashboard Cite

show abstract

“…[ 21 ]. Ruditapes philippinarum has been proven to have antioxidant [ 22 , 23 ], antitumor [ 24 ], hepatoprotective [ 25 ], hypoglycemic [ 26 , 27 ], hypotensive [ 22 ], antimicrobial [ 28 ], anticarcinogenic [ 29 ], immunomodulatory [ 21 ], and other effects, but there are few studies on its anti-inflammatory activity.…”

Section: Introductionmentioning

confidence: 99%

Anti-Inflammatory Activity of Peptides from Ruditapes philippinarum in Lipopolysaccharide-Induced RAW264.7 Cells and Mice

Lin,

Shen,

Jiang

et al. 2024

Foods

View full text Add to dashboard Cite

In our previous study, two peptides with favorable anti-inflammatory effects, Asp-Gln-Thr-Phe (DQTF) and Gly-Tyr-Thr-Arg (GYTR), were screened from Ruditapes philippinarum using an in vitro–in silico strategy. The present study aims to investigate the ameliorative effect of Ruditapes philippinarum peptides (RPPs) on acute inflammation and clarify the potential mechanism through in vitro and in vivo experiments. The anti-inflammatory effects of DQTF and GYTR were verified with a lipopolysaccharide (LPS)-induced RAW264.7 cell acute inflammation model and the anti-inflammatory effect of the enzymatic hydrolysates of Ruditapes philippinarum was explored in vivo using an LPS-induced acute inflammatory injury model in mice. The results show that DQTF and GYTR improved the morphology of LPS-injured cells and decreased the concentrations of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in LPS-induced cells. Moreover, the antioxidant enzyme activity in cells was markedly increased with DQTF and GYTR. The enzymatic hydrolysates of Ruditapes philippinarum were obtained with hydrolysis using pepsin–chymotrypsin–trypsin (PeCTHC) and pepsin–trypsin (PeTHC), respectively. PeCTHC and PeTHC significantly reduced pro-inflammatory cytokines and nitric oxide (NO) in the serum. Additionally, the blood indices and levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and malondialdehyde (MDA) in the livers of mice were markedly improved with RPPs administration. In conclusion, RPPs have preventive and protective effects on acute inflammation, with significant prospects for development in the field of functional foods.

show abstract

Bioactive dipeptides mitigate high-fat and high-fructose corn syrup diet-induced metabolic-associated fatty liver disease via upregulation of Nrf2/HO-1 expressions in C57BL/6J mice

Wayal,

Hsieh

2023

Biomedicine & Pharmacotherapy

View full text Add to dashboard Cite

Preparation and Hepatoprotective Activities of Peptides Derived from Mussels (Mytilus edulis) and Clams (Ruditapes philippinarum)

Cited by 5 publications

References 69 publications

Molecular and biotechnological characteristics of proteolytic activity from <i>Streptococcus thermophilus</i> as a proteolytic lactic acid bacteria to enhance protein-derived bioactive peptides

Molecular and biotechnological characteristics of proteolytic activity from <i>Streptococcus thermophilus</i> as a proteolytic lactic acid bacteria to enhance protein-derived bioactive peptides

Anti-Inflammatory Activity of Peptides from Ruditapes philippinarum in Lipopolysaccharide-Induced RAW264.7 Cells and Mice

Bioactive dipeptides mitigate high-fat and high-fructose corn syrup diet-induced metabolic-associated fatty liver disease via upregulation of Nrf2/HO-1 expressions in C57BL/6J mice

Contact Info

Product

Resources

About