Preparation and Cytotoxicity of Poly(Methyl Methacrylate) Nanoparticles for Drug Encapsulation

Mendes, Anderson Nogueira; Hubber, Isabela; Siqueira, M.I.N.; Barbosa, Gleyce Moreno; Moreira, Davyson de Lima; Holandino, Carla; Pinto, José Carlos; Nele, Márcio

doi:10.1002/masy.201100248

Cited by 26 publications

(21 citation statements)

References 25 publications

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“…These data corroborate with those described in the literature, since NPs containing PMMA are not cytotoxic. 13 Our results indicated that PCA-HEX and PMMA-RML-PCA-HEX induced cell death. The IC 50 value calculated for PMMA-RML-PCA-HEX was 350 μg/mL and for PCA-HEX was 460 μg/mL.…”

mentioning

confidence: 57%

“…The concentrations applied in leukemic cells ranged from 150 μg/mL to 1,500 μg/mL for determination of the minimum inhibitory concentration. Cytotoxicity was determined using trypan blue (TB) and MTT assays, as described by Mendes et al 13 hemolytic activity…”

Section: Cytotoxic Activity Of Polymeric Npsmentioning

confidence: 99%

“…11 However, studies showed that PMMA may have other applications such as in bioplasty 12 and as a carrier for antitumor drugs. 9,13 PMMA NPs are biocompatible and can be produced by miniemulsion, emulsion, and suspension processes. 13,14 For example, PMMA NPs complexed with survivin may act in reducing the proliferation of human A549 cells.…”

Section: Introductionmentioning

confidence: 99%

“…9,13 PMMA NPs are biocompatible and can be produced by miniemulsion, emulsion, and suspension processes. 13,14 For example, PMMA NPs complexed with survivin may act in reducing the proliferation of human A549 cells. 9 Cancer is a leading cause of death worldwide and a major challenge for modern chemotherapy.…”

Section: Introductionmentioning

confidence: 99%

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Encapsulation of <em>Piper cabralanum </em>(Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells

Mendes¹,

Filgueiras²,

Siqueira³

et al. 2017

IJN

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This study aimed to synthesize and characterize nanoparticles (NPs) of poly(methyl methacrylate) (PMMA) and evaluate their ability to incorporate plant extracts with antitumor activity and low dissolution in aqueous media. The extract used was n -hexane partition of the methanol extract of Piper cabralanum (PCA-HEX). PMMA NPs were obtained using the mini-emulsion method, which was able to encapsulate almost 100% of PCA-HEX. The synthesized polymeric particles presented with a size of 200 nm and a negative charge. Cytotoxicity tests by MTT and trypan blue assays showed that NPs without PCA-HEX did not kill leukemic cells (K562 cells). NPs containing PCA-HEX were able to enhance cell death when compared to pure extract. The results showed that PMMA NPs could be useful as a drug delivery system as they can enhance the antitumor activity of the PCA-HEX extract by more than 20-fold. PMMA NPs containing plant extracts with antitumor activities may be an alternative to control the evolution of diseases such as leukemia.

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mentioning

confidence: 57%

Section: Cytotoxic Activity Of Polymeric Npsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Encapsulation of <em>Piper cabralanum </em>(Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells

Mendes¹,

Filgueiras²,

Siqueira³

et al. 2017

IJN

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“…Authors did not detect any changes in anatomopathological, haematological, and biochemical parameters. PMMA nanoparticles have also demonstrated lower toxicity in vitro assays with human cell cultures (K562 [29], TPH1 e A549 [26]). …”

Section: International Journal Of Polymer Sciencementioning

confidence: 99%

Method Validation for Progesterone Determination in Poly(methyl methacrylate) Nanoparticles Synthesized via Miniemulsion Polymerization

Fogolari

Felippe

Leimann

et al. 2017

International Journal of Polymer Science

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Exogenous progesterone has several applications in human health and in veterinary medicine, especially in fixed-time artificial insemination protocol. Progesterone nanoencapsulation in biocompatible polymers, such as poly(methyl methacrylate) (PMMA), is an alternative to substitute silicone-based release device traditionally used for estrus control. Progesterone concentration inside the nanoparticles must be precisely known; for that reason, a validation methodology must be applied to ensure reliable results, suitable for nanoparticles application. In this work, an UV-Vis spectrophotometric method was validated for the determination of progesterone in PMMA nanoparticles synthesized by miniemulsion polymerization. Chloroform was used as solvent, showing selectivity to the encapsulated drug and the components of the polymeric matrix did not influence progesterone recovery. Detection and quantitation limits (DL and QL) obtained were 0.32 and 0.96 mg⋅L −1 , respectively, and precision tests (between different analysts and equipment) indicated acceptable Relative Standard Deviations (RSD < 5%). Miniemulsion polymerization reactions were carried out producing two different morphologies: nanospheres (NS) and nanocapsules (NC), with average intensity diameters (Dz) of 150-200 nm and 240-300 nm, respectively. Polymerization gravimetric conversions obtained for both cases were higher than 95% and encapsulation efficiencies greater than 69% and 90% for the nanospheres and nanocapsules, respectively.

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Synthesis and Characterization of Poly(Methyl Methacrylate) PMMA and Evaluation of Cytotoxicity for Biomedical Application

Feuser

Gaspar

Ricci-Júnior

et al. 2014

Macromolecular Symposia

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Summary: The objective this work was the synthesis, characterization and evaluation of cytotoxicity poly(methyl methacrylate), PMMA, nanoparticles (NPs) produced using miniemulsion polymerization technique adopting azobisisobutylonitrile (AIBN) as initiator and biocompatible and biodegradable surfactant, lecithin and co-stabilizer, miglyol 812 The NPs showed high stability during one year and an average diameter of 89 nm with polydispersity index of 0.11 and a zeta potential À48 mV. The PMMA NPs were tested for cytotoxicity in acute monocytic leukemia cells (THP1) and in human lung adenocarcinoma cells (A549). The PMMA NPs did not show any adverse effect on cell viability indicating their potential to encapsulate antitumor agents.

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Preparation and Cytotoxicity of Poly(Methyl Methacrylate) Nanoparticles for Drug Encapsulation

Cited by 26 publications

References 25 publications

Encapsulation of <em>Piper cabralanum </em>(Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells

Encapsulation of <em>Piper cabralanum </em>(Piperaceae) nonpolar extract in poly(methyl methacrylate) by miniemulsion and evaluation of increase in the effectiveness of antileukemic activity in K562 cells

Method Validation for Progesterone Determination in Poly(methyl methacrylate) Nanoparticles Synthesized via Miniemulsion Polymerization

Synthesis and Characterization of Poly(Methyl Methacrylate) PMMA and Evaluation of Cytotoxicity for Biomedical Application

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