Synthesis and Characterization of Poly(Methyl Methacrylate) PMMA and Evaluation of Cytotoxicity for Biomedical Application

Abstract

Summary: The objective this work was the synthesis, characterization and evaluation of cytotoxicity poly(methyl methacrylate), PMMA, nanoparticles (NPs) produced using miniemulsion polymerization technique adopting azobisisobutylonitrile (AIBN) as initiator and biocompatible and biodegradable surfactant, lecithin and co-stabilizer, miglyol 812 The NPs showed high stability during one year and an average diameter of 89 nm with polydispersity index of 0.11 and a zeta potential À48 mV. The PMMA NPs were tested for cytotoxicity in acute monocytic leukemia cells (THP1) and in human lung adenocarcinoma cells (A549). The PMMA NPs did not show any adverse effect on cell viability indicating their potential to encapsulate antitumor agents.

Simultaneous encapsulation of magnetic nanoparticles and zinc phthalocyanine in poly(methyl methacrylate) nanoparticles by miniemulsion polymerization and in vitro studies

“…The preparation of PMMA nanoparticles via miniemulsion polymerization was the same as described by Feuser et al [30]. The aqueous phase was composed of 20 mL distilled water and the organic phase contained:2 g of MMA (monomer), 0.…”

“…diameter when MNPs and ZnPc were added (from 89 nm for PMMA NPs to 104 nm for MNPsZnPc-PMMA NPs), as it reduced the compartmentalization effect and the total number of free radicals present in the reaction medium[30,34]. Another reason was the reaction of the oxidizing initiator (AIBN) with part of the magnetite (Fe 3 O 4 ) converting it to Fe 2 O 3 reducing the concentration of AIBN from the reaction medium[20,21,34].…”

Method Validation for Progesterone Determination in Poly(methyl methacrylate) Nanoparticles Synthesized via Miniemulsion Polymerization

“…Therefore, the use of biocompatible polymers and solvent-free, natural, and nonionic emulsifiers such as lecithin, in addition of costabilizers based in oils and fatty acids, is preferred for biomedical applications [9,[23][24][25][26].…”

“…Authors did not detect any changes in anatomopathological, haematological, and biochemical parameters. PMMA nanoparticles have also demonstrated lower toxicity in vitro assays with human cell cultures (K562 [29], TPH1 e A549 [26]). …”

“…PMMA nanoparticles were produced by the miniemulsion polymerization technique and the formulations were defined according to previous studies conducted in the research group in the synthesis of biocompatible nanoparticles for drug delivery [23][24][25][26]. Formulations used in particles synthesis are presented in Table 1 for nanospheres (NS) and nanocapsules (NC).…”