Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)

Chen, Junde; Wang, Guangyu; Li, Yushuang

doi:10.3390/md19110597

Cited by 11 publications

(10 citation statements)

References 54 publications

(118 reference statements)

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“…Relative to dry swim bladder weight, the respective yields of ASC and PSC were 33.38% and 84.79%, suggesting that pepsin was able to markedly enhance extraction efficiency, in line with prior reports [ 15 , 16 , 17 ]. This is at least partially attributable to the covalent telopeptide crosslinking of collagen molecules, as pepsin can cleave telopeptide residues to enhance overall collagen solubility [ 18 ].…”

Section: Resultssupporting

confidence: 90%

Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca

Wang

et al. 2023

Marine Drugs

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There is a growing demand for the identification of alternative sources of collagen not derived from land-dwelling animals. The present study explored the use of pepsin- and acid-based extraction protocols to isolate collagen from the swim bladders of Megalonibea fusca. After extraction, these acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) samples respectively were subjected to spectral analyses and sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) characterization, revealing both to be comprised of type I collagen with a triple-helical structure. The imino acid content of these ASC and PSC samples was 195 and 199 residues per 1000 residues, respectively. Scanning electron microscopy demonstrated that samples of freeze-dried collagen exhibited a compact lamellar structure, while transmission electron microscopy and atomic force microscopy confirmed the ability of these collagens to undergo self-assembly into fibers. ASC samples exhibited a larger fiber diameter than the PSC samples. The solubility of both ASC and PSC was highest under acidic pH conditions. Neither ASC nor PSC caused any cytotoxicity when tested in vitro, which met one of the requirements for the biological evaluation of medical devices. Thus, collagen isolated from the swim bladders of Megalonibea fusca holds great promise as a potential alternative to mammalian collagen.

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Section: Resultssupporting

confidence: 90%

Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca

Wang

et al. 2023

Marine Drugs

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“…38 Further, the solubility of the samples gradually increased with an increase in pH from 7 to 11 (p < 0.001) due to the net negative charge on protein or peptides caused by the dif ference in pH and pI values. 39 Although the solubility pattern of P coll. and PSCH is similar, it is slightly higher for PSCH than P coll.…”

Section: Solubility Of P Coll and Pschmentioning

confidence: 99%

“…40 The results were in agreement with other reports that suggested hydrolysates have better solubility than collagen at different pH's. 39,41,42 Surface microstructure of P coll. and PSCH…”

Section: Solubility Of P Coll and Pschmentioning

confidence: 99%

Biophysical and in vitro wound healing assessment of collagen peptides processed from fish skin waste

Acharya

Bhat

et al. 2022

Journal of Bioactive and Compatible Polymers

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The present study was conducted to examine the bioactive and wound healing properties of collagen hydrolysate derived from Piaractus brachypomus (pacu) fish skin waste. Collagen type I (P coll.) yielding 72.25% was isolated from skin waste by following acid-soluble collagen extraction method. Further, collagen was fragmented using bacterial collagenase and the processed collagen hydrolysate (peptides) was in the range of 10–15 kDa that was further purified using ion-exchange chromatography. The FTIR spectra of both P coll. and collagen hydrolysate (PSCH) were nearly similar showing that PSCH retained the structural and chemical composition similar to its parent molecule (P coll.). Solubility analysis revealed that PSCH has slightly better solubility compared to P coll. Similarly, scanning electron micrographs also exhibited more uniform and porous microstructure of PSCH compared to P coll. Further, PSCH was found to be efficient in peroxide quenching (64.5%) and radical scavenging activities (85.74%). MTT studies confirmed PSCH to be non-toxic displaying 84.68% cell viability at the highest concentration (3 mg/ml) and hemocompatibility test revealed PSCH to be non-hemolytic with minimal lysis of only 2.1% of human RBCs. In addition, PSCH also displayed a remarkable wound closure ability of more than 80% at 12 h and 100% within 24 h. Hence, these findings suggest that recycled PSCH has potent wound healing ability and can be produced economically on a large scale for possible biological applications in regenerative medicine.

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“…Wang et al separated types I and V collagens from the skin of deep-sea redfish by chromatographic techniques [ 7 ]. Chen et al separated type I collagen from the scales of the lizardfish and maintained the triple-helical structures with no cytotoxicity [ 8 ]. Recently, some Japanese scholars extracted collagen from the scales of barramundi ( Lates calcarifer ), which was found to be comparable to that of mammals and showed potential for three-dimensional cell cultivation [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

Yang

Chen

2022

Marine Drugs

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Marine collagen is an ideal material for tissue engineering due to its excellent biological properties. However, the limited mechanical properties and poor stability of marine collagen limit its application in tissue engineering. Here, collagen was extracted from the skin of tilapia (Oreochromis nilotica). Collagen-thermoplastic polyurethane (Col-TPU) fibrous membranes were prepared using tilapia collagen as a foundational material, and their physicochemical and biocompatibility were investigated. Fourier transform infrared spectroscopy results showed that thermoplastic polyurethane was successfully combined with collagen, and the triple helix structure of collagen was retained. X-ray diffraction and differential scanning calorimetry results showed relatively good compatibility between collagen and TPU.SEM results showed that the average diameter of the composite nanofiber membrane decreased with increasing thermoplastic polyurethane proportion. The mechanical evaluation and thermogravimetric analysis showed that the thermal stability and tensile properties of Col-TPU fibrous membranes were significantly improved with increasing TPU. Cytotoxicity experiments confirmed that fibrous membranes with different ratios of thermoplastic polyurethane content showed no significant toxicity to fibroblasts; Col-TPU fibrous membranes were conducive to the migration and adhesion of cells. Thus, these Col-TPU composite nanofiber membranes might be used as a potential biomaterial in tissue regeneration.

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Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (Synodus macrops)

Cited by 11 publications

References 54 publications

Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca

Extraction and Characterization of Pepsin- and Acid-Soluble Collagen from the Swim Bladders of Megalonibea fusca

Biophysical and in vitro wound healing assessment of collagen peptides processed from fish skin waste

Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

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