Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

Wu, Sijia; Yang, Likun; Chen, Junde

doi:10.3390/md20070437

Cited by 2 publications

(1 citation statement)

References 59 publications

(64 reference statements)

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“…The SDS-PAGE results presented in figure 5 confirmed the presence of high-intensity bands corresponding to the α1 and α2 chains. Notably, the α1 chain exhibited a two-fold higher intensity compared to the α2 chain, suggesting that type I collagen is the predominant protein component within the dECM samples [19,50,51]. The observed preservation of high molecular weight protein fractions, particularly the α1 and α2 chains, further supports the retention of type I collagen's structural and functional integrity within the dECM.…”

Section: Discussionmentioning

confidence: 73%

Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction

Arellano,

Bual,

Bantilan

et al. 2024

Mater. Res. Express

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Tilapia scales, typically regarded as low-value biological waste, possess valuable organic components such as collagen and inorganic components such as hydroxyapatite. Converting these wastes into functional biomaterials like decellularized extracellular matrix (dECM) contributes to long-term fish waste management to achieve sustainable fish consumption and promote a circular economy by creating a product with a high market value. Triton X-100 (TX-100) and Sodium-dodecyl sulfate (SDS) at varying concentrations (0.1%, 0.5%, and 1%) are utilized to extract the extracellular matrix. The study aims to evaluate the effectiveness of these agents in decellularizing the scales and producing a suitable dECM scaffold. Histological analysis using H&E staining revealed a significant decrease in cellular components in the decellularized samples. This was supported by the dsDNA quantification results showing the highest removal rate of 96% in the samples treated with 1% SDS. Attenuated Total Reflectance-Fourier-transform infrared (ATR-FTIR) spectra showed the amide peaks (Amide A, B, I, II, and III) indicating the presence of type I collagen. The confirmation of type I collagen was further supported by the analysis of SDS-PAGE images, which displayed the presence of α1 and α2 chains, β-dimer, and the γ-band. Among the dECM, samples treated with 0.1% TX-100 exhibited the highest protein content, averaging 593.33±17.78 µg/mg while 1% SDS showed the lowest protein content averaging 61.33±24.03µg/mg. All dECM samples demonstrated protein denaturation temperatures ranging from 70-75°C. This study presents baseline data of the single chemical decellularization treatment method utilized to extract ECM from tilapia scales and its resulting dECM. Further research is recommended to assess the biocompatibility and cytotoxicity of the produced dECM and explore its potential applications.

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Section: Discussionmentioning

confidence: 73%

Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction

Arellano,

Bual,

Bantilan

et al. 2024

Mater. Res. Express

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Application of customisable 3D-printed bite blocks combined with nano head, neck and shoulder mask to head and neck neoplasm radiotherapy

Zheng,

Li,

Zhu

et al. 2023

mat express

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This study investigated the effectiveness of customisable 3D-printed bite blocks combined with nano head, neck and shoulder mask in radiotherapy for head and neck neoplasms. The images of 50 patients with head and neck neoplasms treated at our hospital from 2020 to 2022 were collected using CBCT. The patients were divided into two groups: 25 in the mask group and 25 in the combination group. The mask group utilized thermoplastic polyurethane (TPU) nanofiber membrane to stabilize the position of the patients’ head, shoulder, and neck. On the other hand, the combined group added a personalized bite block produced by 3D printing technology on the basis of the mask group. Cone beam CT (CBCT) was used to calibrate any errors, and the set-up errors and number of head rotations after image calibration were analyzed between the two groups. The conformation number (CN) and heterogeneity index (HI) in the target area of the intensity-modulated radiation therapy (IMRT) plan of the two groups were calculated, and the dose-volume parameters of the tongue and other organs at risk, including Dmax and Dmean, were compared using paired tests. Follow-up observations were performed to assess the occurrence of adverse reactions to radiation damage among the patients. The findings revealed a statistically significant difference in the Y -axis set-up error between the combination group and the mask group (P < 0.05). Additionally, the combination group exhibited a higher proportion of set-up errors < 2 mm in both the Y -axis and Z-axis directions compared to the mask group. Significantly fewer images showed head rotation in the combination group than in the mask group. In terms of the target area, there were no significant differences observed in the conformation number (CN) and heterogeneity index (HI) between the two groups. However, wearing the personalized bite block resulted in significantly lower Dmax and Dmean values for the tongue. In contrast, the mask group experienced a higher incidence of oral mucositis and taste disorders. By utilizing customizable 3D-printed bite blocks in conjunction with a nano-mask, accurate repositioning for chemotherapy was achieved, leading to a reduction in set-up error and a significant decrease in radiation dose to the tongue and its surface oral mucosa. Consequently, the occurrence of adverse reactions such as oral mucositis and taste disorders was also reduced. The production of personalized bite blocks is simple, with accurate radiotherapy, making it a technique that should be widely adopted.

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Preparation and Characterization of Tilapia Collagen-Thermoplastic Polyurethane Composite Nanofiber Membranes

Cited by 2 publications

References 59 publications

Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction

Upcycling waste tilapia (oreochromis niloticus) scales through a decellularization process for extracellular matrix extraction

Application of customisable 3D-printed bite blocks combined with nano head, neck and shoulder mask to head and neck neoplasm radiotherapy

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