Preparation and Characterization of the Enzymatic Degradation Products of the Exopolysaccharide FromKlebsiellaK13

Abstract

Oligosaccharides were prepared from the exopolysaccharide of Klebsiella K13 by enzymatic degradation and characterized. A phage-borne depolymerase enzyme was used to degrade the exopolysaccharide of Klebsiella K13. Bio-Gel P4 and P6 were used to purify the oligosaccharide products. The purified oligosaccharides were characterized by HPLC, mass spectroscopy, infrared spectroscopy, and NMR spectroscopy. The monosaccharide constituents of these enzymatic degradation products include D-glucose, D-galactose, D-mannose, and D-glucuronic acid. It was concluded that a pentasaccharide repeating unit with the following structure, as well as its dimer and trimer, was released from the exopolysaccharide: 3,4-O-(1-carboxyethylidene)-β-D-Galp-(1→4)-α-DGlcpA-(1→3)-β-D-Manp-(1→4)-α-D-Glcp-(1→3)-D-Glcp

Fucose-containing bacterial exopolysaccharides: Sources, biological activities, and food applications

“…sp. strains have been characterized ( Rieger-Hug and Stirm, 1981 , Shang et al, 2014 ). Moreover, two FCOs with new structures were successfully obtained via degradation of the FcEPS produced by E. sakazakii M1 by bacteriophage-borne glycanase ( Xiao et al, 2021 ).…”