Preparation and Characterization of Selenium Incorporated Guar Gum Nanoparticle and Its Interaction with H9c2 Cells

Abstract

This study deals with the preparation and characterization of selenium incorporated guar gum nanoparticle (SGG), and its effect on H9c2 cardiomyoblast. Herein, nanoprecipitation techniques had been employed for the preparation of SGG nanoparticle. The prepared nanoparticle had been subjected to various types of analytical techniques like transmission electron microscopy (TEM), X-ray diffraction (XRD) and particle size analysis to confirm the characteristics of nanoparticle as well as for selenium incorporation. Physical characterization of nanoparticle showed that the size of nanoparticles increase upto ∼69–173 nm upon selenium incorporation from ∼41–132 nm. Then the prepared nanoparticles were evaluated for its effect on H9c2 cells. In this regard, the effect of nanoparticle on various vital parameters of H9c2 cells was studied. Parameters like cell viability, uptake of selenium incorporated guar gum nanoparticle by the cells, effect of SGG on DNA integrity, apoptosis, reactive oxygen species generation, alteration in transmembrane potential of mitochondria and cytoskeletal integrity had been investigated. Viability results showed that up to 25 nM of SGG was safe (10.31%) but beyond that it induces cytotoxicity. Cellular uptake of selenium showed that cell permeability for SGG is significantly high compared to normal selenium (7.2 nM of selenium for 25 nM SGG compared with 5.2 nM selenium for 25 nM sodium selenite). There was no apoptosis with SGG and also it protects DNA from hydroxyl radical induced breakage. Likewise no adverse effect on mitochondria and cytoskeleton was observed for 25 nM of SGG. Overall results reveal that SGG is highly suitable for biomedical research application.

Synthesis, characterization, and controlled release of selenium nanoparticles stabilized by chitosan of different molecular weights

“…4c and d, which were consistent with a previous report demonstrating that CS was conjugated to SeNPs through −OH groups (Yu et al, 2012). Further supports for the above conclusion comes from recent studies showing that selenium was prone to interacted with O-and N-containing groups by forming Se-O and Se-N bonds, consequently stabilizing selenium nanoparticles by other dispersants Soumya et al, 2013). In sum, the synthesis of CS-SeNPs might begin with an attack of the precursor, SeO 3 2− by the amino groups of CS because of electrostatic effects.…”

“…Many efforts have been made to stabilize the red selenium particles including the use of proteins (Kaur, Iqbal, & Bakshi, 2009;Shirazi et al, 2014;Xia, 2007), polyphenols (Barnaby, Sarker, Dowdell, & Bannerjee, 2011;Pi et al, 2013;Wu et al, 2013), polysaccharides (Kong et al, 2014;Soumya, Vineetha, Reshma, & Raghu, 2013;Zheng et al, 2013), melatonin , ATP , and monosaccharides (Zheng et al, 2011). These all have been used as dispersants to prepare stable and uniform selenium nanoparticles.…”

Sodium selenite/selenium nanoparticles (SeNPs) protect cardiomyoblasts and zebrafish embryos against ethanol induced oxidative stress

“…Exposure of H9C2 cells to certain chemicals for 24 hrs leads to apoptosis [8]. Treatment with 100 nM sodium selenite and SeNPs are effective in protecting the cells from apoptosis at 24 hrs and this property of selenium and its nanoparticles indicate their potential usefulness as a therapeutic agent against cardiomyopathy ( Figure.8).…”

Preparation and antioxidant properties of selenium nanoparticles-loaded chitosan microspheres

“…Over the past decade, much effort had been devoted to synthesize and stabilize SeNPs with the use of polysaccharides, 15,[18][19][20] monosaccharides, 21 proteins, 3,[22][23][24] amino acids, 25 polyphenols, [26][27][28] melatonin, 29 ATP, 30 complex plant extract 10,31 and even microorganism culture. 8 Among them, polysaccharide is regarded as an appropriate template for fabricating SeNPs when considering energy efficiency and eco-friendliness, according to Zhang et al 15 However, the SeNPs prepared using polysaccharides were found to be unstable, due to enlargement of size and decrease of zeta potential during storage in aqueous environment.…”