Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa

Wang, Wei; Zhang, Xia; Chao, Ningning; Qin, Tingwu; Ding, Wei; Zhang, Yi; Sang, Jiangwei; Luo, Jingcong

doi:10.1016/j.actbio.2015.10.013

Cited by 77 publications

(66 citation statements)

References 65 publications

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“…[55] Recently, the in vivo angiogenic potential of pSIS bioscaffold hydrogels was associated with matrix degradation-dependent release of FGF2 and VEGF. [13] Likewise, Adv ECM bioscaffold-derived hydrogels serve as a depot for signals such as FGF2 that influence cell behaviors important for blood vessel formation.…”

Section: Discussionmentioning

confidence: 99%

“…Although ECM hydrogels from a variety of tissue sources exhibited inherent bioactivity, [46-50] investigation of their impact on angiogenesis emerged only recently. [13, 25] These studies provided evidence that ECM bioscaffolds influence [56, 57] and interact with [18, 39] blood vessel cells. The angiogenic potential of hybrid scaffolds has been better studied using synthetic materials conjugated with unique combinations of tissue-derived angiogenic growth factors and must be precisely engineered for specific applications with engineered growth factor content and release profiles.…”

Section: Discussionmentioning

confidence: 99%

“…Growth factors including transforming growth factor-beta, basic fibroblast growth factor (FGF), hepatocyte growth factor and vascular endothelial growth factor (VEGF) persist in their bioactive form within ECM bioscaffolds after sterilization. [13-17] Additionally, degradation of ECM bioscaffolds releases matricryptic peptides that invoke biologic activity. [18] ECM bioscaffolds guide stem cell differentiation through growth factor retention and unique matrix compliance, [19-23] which together comprise tissue-specific microenvironments that are advantageous for regeneration.…”

Section: Introductionmentioning

confidence: 99%

“…[13, 25] Although the vasculogenic and angiogenic mechanisms of ECM bioscaffolds are not fully understood, gradual release of growth factors during ECM degradation is a likely mechanism of action. [13] Since immobilized growth factors secreted by the resident cells fortify ECM, [26, 27] vascular ECM is a viable candidate biomaterial for invoking vasculogenesis and angiogenesis. The adventitia of blood vessels is a perivascular microenvironment that is heterogeneous in both form and function.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

et al. 2017

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Extracellular matrix (ECM)-derived bioscaffolds have been shown to elicit tissue repair through retention of bioactive signals. Given that the adventitia of large blood vessels is a richly vascularized microenvironment, we hypothesized that perivascular ECM contains bioactive signals that influence cells of blood vessel lineages. ECM bioscaffolds were derived from decellularized human and porcine aortic adventitia (hAdv and pAdv, respectively) and then shown have minimal DNA content and retain elastin and collagen proteins. Hydrogel formulations of hAdv and pAdv ECM bioscaffolds exhibited gelation kinetics similar to ECM hydrogels derived from porcine small intestinal submucosa (pSIS). hAdv and pAdv ECM hydrogels displayed thinner, less undulated, and fibrous microarchitecture reminiscent of native adventitia, with slight differences in ultrastructure visible in comparison to pSIS ECM hydrogels. Pepsin-digested pAdv and pSIS ECM bioscaffolds increased proliferation of human adventitia-derived endothelial cells and this effect was mediated in part by basic fibroblast growth factor (FGF2). Human endothelial cells cultured on Matrigel substrates formed more numerous and longer tube-like structures when supplemented with pAdv ECM bioscaffolds, and FGF2 mediated this matrix signaling. ECM bioscaffolds derived from pAdv promoted FGF2-dependent in vivo angiogenesis in the chick chorioallantoic membrane model. Using an angiogenesis-focused protein array, we detected 55 angiogenesis-related proteins, including FGF2 in hAdv, pAdv and pSIS ECMs. Interestingly, 19 of these factors were less abundant in ECMs bioscaffolds derived from aneurysmal specimens of human aorta when compared with nonaneurysmal (normal) specimens. This study reveals that Adv ECM hydrogels recapitulate matrix fiber microarchitecture of native adventitia, and retain angiogenesis-related actors and bioactive properties such as FGF2 signaling capable of influencing processes important for angiogenesis. This work supports the use of Adv ECM bioscaffolds for both discovery biology and potential translation towards microvascular regeneration in clinical applications.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

et al. 2017

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“…The thermosensitive and gelation property of decellularized ECM biomaterials has been widely recognized. 24,25 For DPAT and other ECM-derived hydrogel, the main component is type I collagen. Type I collagen consists of (a1) 2 a2 biopolymer in the form of triple helix.…”

Section: Discussionmentioning

confidence: 99%

Hydrogel derived from decellularized porcine adipose tissue as a promising biomaterial for soft tissue augmentation

Tan

Zhang

Luo

et al. 2017

J Biomedical Materials Res

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Decellularized extracellular matrix (ECM) scaffolds from human adipose tissue, characterized by impressive adipogenic induction ability, are promising for soft tissue augmentation. However, scaffolds from autologous human adipose tissue are limited by the availability of tissue resources and the time necessary for scaffold fabrication. The objective of the current study was to investigate the adipogenic properties of hydrogels of decellularized porcine adipose tissue (HDPA). HDPA induced the adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro, with significantly increased expression of adipogenic genes. Subcutaneous injection of HDPA in immunocompetent mice induced host-derived adipogenesis without cell seeding, and adipogenesis was significantly enhanced with ADSCs seeding. The newly formed adipocytes were frequently located on the basal side in the non-seeding group, but this trend was not observed in the ADSCs seeding group. Our results indicated that, similar to human adipose tissue, the ECM scaffold derived from porcine adipose tissue could provide an adipogenic microenvironment for adipose tissue regeneration and is a promising biomaterial for soft tissue augmentation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1756-1764, 2017.

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Photo‐Responsive Decellularized Small Intestine Submucosa Hydrogels

Duong,

Nguyen,

Luong

et al. 2024

Adv Funct Materials

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Decellularized small intestine submucosa (dSIS) is a promising biomaterial for promoting tissue regeneration. Isolated from the submucosal layer of animal jejunum, SIS is rich in extracellular matrix (ECM) proteins, including collagen, laminin, and fibronectin. Following mild decellularization, dSIS becomes an acellular matrix that supports cell adhesion, proliferation, and differentiation. Conventional dSIS matrix is usually obtained by thermal crosslinking, which yields a soft scaffold with low stability. To address these challenges, dSIS is modified with methacrylate groups for photocrosslinking into stable hydrogels. However, dSIS has not been modified with clickable handles for orthogonal crosslinking. Here, the development of norbornene‐modified dSIS, named dSIS‐NB, via reacting amine groups of dSIS with carbic anhydride in acidic aqueous reaction conditions is reported. Using triethylamine (TEA) as a mild base catalyst, high degrees of NB substitution on dSIS are obtained. In addition to describing the synthesis of dSIS‐NB, its adaptability in orthogonal hydrogel crosslinking for cancer and vascular tissue engineering is explored. Impressively, compared with physically crosslinked dSIS and collagen matrices, orthogonally crosslinked dSIS‐NB hydrogels supported rapid dissemination of cancer cells and superior vasculogenic and angiogenic properties. dSIS‐NB is also exploited as a versatile bioink for 3D bioprinting.

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Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa

Cited by 77 publications

References 65 publications

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

Perivascular extracellular matrix hydrogels mimic native matrix microarchitecture and promote angiogenesis via basic fibroblast growth factor

Hydrogel derived from decellularized porcine adipose tissue as a promising biomaterial for soft tissue augmentation

Photo‐Responsive Decellularized Small Intestine Submucosa Hydrogels

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