Hydrogel derived from decellularized porcine adipose tissue as a promising biomaterial for soft tissue augmentation

Abstract

Decellularized extracellular matrix (ECM) scaffolds from human adipose tissue, characterized by impressive adipogenic induction ability, are promising for soft tissue augmentation. However, scaffolds from autologous human adipose tissue are limited by the availability of tissue resources and the time necessary for scaffold fabrication. The objective of the current study was to investigate the adipogenic properties of hydrogels of decellularized porcine adipose tissue (HDPA). HDPA induced the adipogenic differentiation of human adipose-derived stem cells (ADSCs) in vitro, with significantly increased expression of adipogenic genes. Subcutaneous injection of HDPA in immunocompetent mice induced host-derived adipogenesis without cell seeding, and adipogenesis was significantly enhanced with ADSCs seeding. The newly formed adipocytes were frequently located on the basal side in the non-seeding group, but this trend was not observed in the ADSCs seeding group. Our results indicated that, similar to human adipose tissue, the ECM scaffold derived from porcine adipose tissue could provide an adipogenic microenvironment for adipose tissue regeneration and is a promising biomaterial for soft tissue augmentation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1756-1764, 2017.

Hydrogel from Acellular Porcine Adipose Tissue Accelerates Wound Healing by Inducing Intradermal Adipocyte Regeneration

“…We fabricated adipose ECM-derived HAPA (Tan et al, 2017) that was transparent and white in color. Gelation was induced via incubation at 37 C for 15 minutes (Figure 1a and b), with the injectability of HAPA facilitating its clinical application in wound treatment.…”

“…Fresh porcine adipose back tissue, which was harvested within 4 hours of animal death, was obtained from a nearby market after coordination with local butchers. HAPA was fabricated according to a previously described method (Tan et al, 2017). Briefly, porcine adipose tissue was homogenized and decellularized with 0.5% SDS and isopropanol for 4 hours and 2 hours, respectively.…”

“…Based on the important role of intradermal adipocytes, their restoration is considered a promising method for enhanced wound healing; however, whether promotion of adipocyte regeneration in the wound bed can effectively improve wound healing remains largely unknown. In our previous study, we showed that hydrogels derived from acellularized porcine adipose tissue (HAPA) can induce adipogenesis of adipose-derived stem cells (ADSCs) after being injected subcutaneously (Tan et al, 2017). HAPA is a promising biomaterial that can be used to induce adipocyte regeneration in the wound bed and thereby contribute to wound healing.…”

Hydrogels in adipose tissue engineering—Potential application in post‐mastectomy breast regeneration

“…The principle component of DAT is type I collagen. During pepsin and acid digestion, this biopolymer can be degraded into monomers, which at a suitable pH and temperature can be polymerised to create a hydrogel network (Tan et al, ). The ability to alter the mechanical properties of these biomaterials adds to their attractiveness for adipose tissue engineering (Table ; Van Vlierberghe et al, ; Zhu & Marchant, ).…”

“…However, the yield of DAT from 100 ml of adipose tissue is small, approximately 10 ml or 1 g, which falls far short of clinical demand. Tan et al () proposed a solution to this limitation by demonstrating the ability of porcine DAT hydrogel to induce adipogenesis of human ADSCs in vitro and to induce host‐derived adipogenesis in vivo. This is a potential xenogenic alternative to human DAT in adipose tissue engineering, with another study by Choi et al () showing similar findings.…”